##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AspEI AviII
BamHI BbrPI BcgI BciVI BfrI
BglII BsaAI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BstBI BstEII BstPI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FseI
FspI HindIII HpaI KspI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 4120 GTATAC GT^[AC][GT]AC
AccII 1004 CGCG CG^CG
AcsI 4162 GAATTT [GA]^AATT[CT]
AflIII 302 ACGTGT A^C[GA][CT]GT
ApoI 4162 GAATTT [GA]^AATT[CT]
AseI 3768 ATTAAT AT^TAAT
AsnI 3768 ATTAAT AT^TAAT
Asp700I 3379 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1378 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 355 TGGCCA TGG^CCA
BbsI 371 GTCTTC GAAGAC(2/6)
BlpI 3161 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3161 GCTCAGC GC^T[ACGT]AGC
BpuAI 371 GTCTTC GAAGAC(2/6)
BsaBI 1933 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 824 CGACCG CG[GA][CT]^CG
Bsp1407I 3083 TGTACA T^GTACA
BsrGI 3083 TGTACA T^GTACA
BssHII 1003 GCGCGC G^CGCGC
BssSI 114 CTCGTG CACGAG(-5/-1)
Bst1107I 4120 GTATAC GTA^TAC
BstUI 1004 CGCG CG^CG
BstZ17I 4120 GTATAC GTA^TAC
CelII 3161 GCTCAGC GC^T[ACGT]AGC
DraI 4167 TTTAAA TTT^AAA
DraIII 77 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 3329 ATGCAT ATGCA^T
MamI 1933 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 355 TGGCCA TGG^CCA
MscI 355 TGGCCA TGG^CCA
MvnI 1004 CGCG CG^CG
NdeI 2499 CATATG CA^TATG
NsiI 3329 ATGCAT ATGCA^T
PflFI 1378 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3768 ATTAAT AT^TAAT
SapI 3403 GCTCTTC GCTCTTC(1/4)
SexAI 3695 ACCAGGT A^CC[AT]GGT
SmaI 2541 CCCGGG CCC^GGG
SmlI 2889 CTCAAG C^T[CT][GA]AG
SspBI 3083 TGTACA T^GTACA
Tth111I 1378 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2541 CCCGGG C^CCGGG
XmaI 2541 CCCGGG C^CCGGG
XmnI 3379 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Alw44I 1518 GTGCAC G^TGCAC
1920 GTGCAC
ApaLI 1518 GTGCAC G^TGCAC
1920 GTGCAC
AvaI 2462 CTCGGG C^[CT]CG[GA]G
2541 CCCGGG
AvrII 1777 CCTAGG C^CTAGG
3229 CCTAGG
BclI 3021 TGATCA T^GATCA
4388 TGATCA
BlnI 1777 CCTAGG C^CTAGG
3229 CCTAGG
BsaI 795 GGTCTC GGTCTC(1/5)
4309 GGTCTC
BsoBI 2462 CTCGGG C^[CT]CG[GA]G
2541 CCCGGG
BsrBI 2249 CCGCTC CCGCTC(-3/-3)
2276 GAGCGG
BsrFI 2940 ACCGGC [GA]^CCGG[CT]
3516 ACCGGC
BstYI 1550 AGATCC [GA]^GATC[CT]
2582 GGATCT
Cfr10I 2940 ACCGGC [GA]^CCGG[CT]
3516 ACCGGC
FbaI 3021 TGATCA T^GATCA
4388 TGATCA
MaeII 302 ACGT A^CGT
681 ACGT
MflI 1550 AGATCC [GA]^GATC[CT]
2582 GGATCT
SacI 2459 GAGCTC GAGCT^C
4028 GAGCTC
SfiI 3067 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3432 GGCCTTAAAGGCC
SphI 3157 GCATGC GCATG^C
4128 GCATGC
SspI 3833 AATATT AAT^ATT
4341 AATATT
XhoII 1550 AGATCC [GA]^GATC[CT]
2582 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI Asp718I
AspHI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI KpnI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI