##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AgeI AhdI AscI
AseI AsnI Asp718I AspEI AvrII
BbeI BlnI BsaBI BsiWI Bsp1407I
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstZ17I CpoI DraI
DraIII Eam1105I EcoRV EcoT22I FseI
HindIII HpaI KasI KpnI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SbfI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1709 GACGTC GACGT^C
AccIII 489 TCCGGA T^CCGGA
AflII 791 CTTAAG C^TTAAG
Aor51HI 3044 AGCGCT AGC^GCT
Asp700I 1729 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1991 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1506 TGCGCA TGC^GCA
BbrPI 2546 CACGTG CAC^GTG
BciVI 2986 GGATAC GTATCC(6/5)
BfrI 791 CTTAAG C^TTAAG
BsaI 854 GAGACC GGTCTC(1/5)
BsaWI 489 TCCGGA [AT]^CCGG[AT]
BseAI 489 TCCGGA T^CCGGA
BsmI 1277 GAATGC GAATGC(1/-1)
BspDI 3633 ATCGAT AT^CGAT
BspEI 489 TCCGGA T^CCGGA
BssHII 204 GCGCGC G^CGCGC
ClaI 3633 ATCGAT AT^CGAT
DrdI 3920 GACTCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 171 CGGCCG C^GGCCG
EclXI 171 CGGCCG C^GGCCG
Eco47III 3044 AGCGCT AGC^GCT
Eco52I 171 CGGCCG C^GGCCG
EcoRI 4376 GAATTC G^AATTC
FspI 1506 TGCGCA TGC^GCA
HincII 1601 GTCGAC GT[CT]^[GA]AC
HindII 1601 GTCGAC GT[CT]^[GA]AC
MroI 489 TCCGGA T^CCGGA
PaeR7I 4325 CTCGAG C^TCGAG
PflFI 1991 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2546 CACGTG CAC^GTG
PmlI 2546 CACGTG CAC^GTG
SalI 1601 GTCGAC G^TCGAC
SapI 1732 GCTCTTC GCTCTTC(1/4)
ScaI 1617 AGTACT AGT^ACT
SexAI 3818 ACCTGGT A^CC[AT]GGT
SfiI 2022 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4173 CCCGGG CCC^GGG
SspI 3325 AATATT AAT^ATT
Tth111I 1991 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1238 CCAGCCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4325 CTCGAG C^TCGAG
XmaCI 4173 CCCGGG C^CCGGG
XmaI 4173 CCCGGG C^CCGGG
XmnI 1729 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 1601 GTCGAC GT^[AC][GT]AC
2228 GTAGAC
Alw44I 2155 GTGCAC G^TGCAC
2749 GTGCAC
ApaLI 2155 GTGCAC G^TGCAC
2749 GTGCAC
BamHI 642 GGATCC G^GATCC
1026 GGATCC
BbsI 3510 GAAGAC GAAGAC(2/6)
980 GTCTTC
BcgI 751 CGAGATTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3731 CGAGGACCCTGC
BclI 501 TGATCA T^GATCA
2757 TGATCA
BglII 1224 AGATCT A^GATCT
2185 AGATCT
BpuAI 3510 GAAGAC GAAGAC(2/6)
980 GTCTTC
BsaAI 1437 CACGTA [CT]AC^GT[GA]
2546 CACGTG
FbaI 501 TGATCA T^GATCA
2757 TGATCA
KspI 23 CCGCGG CCGC^GG
162 CCGCGG
PshAI 938 GACGTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3565 GACACACGTC
SacI 236 GAGCTC GAGCT^C
773 GAGCTC
SacII 23 CCGCGG CCGC^GG
162 CCGCGG
SphI 1016 GCATGC GCATG^C
2676 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII