##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AviII BbrPI BciVI
BfrI BsaAI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrGI BssHII BssSI BstBI
ClaI CpoI Eco47III EcoRI EcoRV
FseI FspI HincII HindII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I RsrII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 1434 ACATGT A^C[GA][CT]GT
AhdI 1215 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1215 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4417 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3583 CCTAGG C^CTAGG
BbsI 3496 GTCTTC GAAGAC(2/6)
BclI 552 TGATCA T^GATCA
BglII 768 AGATCT A^GATCT
BlnI 3583 CCTAGG C^CTAGG
BlpI 706 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 706 GCTCAGC GC^T[ACGT]AGC
BpuAI 3496 GTCTTC GAAGAC(2/6)
BspLU11I 1434 ACATGT A^CATGT
Bst1107I 635 GTATAC GTA^TAC
BstZ17I 635 GTATAC GTA^TAC
CelII 706 GCTCAGC GC^T[ACGT]AGC
DraI 4737 TTTAAA TTT^AAA
DrdI 1600 GACTGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 283 CGGCCG C^GGCCG
Eam1105I 1215 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 283 CGGCCG C^GGCCG
Eco52I 283 CGGCCG C^GGCCG
FbaI 552 TGATCA T^GATCA
HindIII 2677 AAGCTT A^AGCTT
NheI 205 GCTAGC G^CTAGC
PflFI 4417 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2154 CGATCG CGAT^CG
SapI 4292 GAAGAGC GCTCTTC(1/4)
SexAI 2564 ACCAGGT A^CC[AT]GGT
SmaI 4153 CCCGGG CCC^GGG
SpeI 1783 ACTAGT A^CTAGT
SphI 643 GCATGC GCATG^C
Tth111I 4417 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4153 CCCGGG C^CCGGG
XmaI 4153 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 910 AAATTT [GA]^AATT[CT]
1122 GAATTT
Alw44I 3685 GTGCAC G^TGCAC
4539 GTGCAC
ApaLI 3685 GTGCAC G^TGCAC
4539 GTGCAC
ApoI 910 AAATTT [GA]^AATT[CT]
1122 GAATTT
AvaI 2436 CTCGGG C^[CT]CG[GA]G
4153 CCCGGG
BcgI 292 CGACGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
433 GCATGGAGGTCG
BsiEI 283 CGGCCG CG[GA][CT]^CG
2154 CGATCG
BsmI 853 GCATTC GAATGC(1/-1)
2727 GCATTC
BsoBI 2436 CTCGGG C^[CT]CG[GA]G
4153 CCCGGG
BstAPI 643 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2016 GCAGACAGTGC
MaeII 464 ACGT A^CGT
662 ACGT
NaeI 3002 GCCGGC GCC^GGC
4101 GCCGGC
NcoI 953 CCATGG C^CATGG
2495 CCATGG
NgoMIV 3002 GCCGGC G^CCGGC
4101 GCCGGC
PshAI 2187 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4038 GACATGGGTC
SacI 2335 GAGCTC GAGCT^C
3812 GAGCTC
TfiI 1884 GAATC G^A[AT]TC
2085 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaII BalI BamHI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII