##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII Alw44I ApaLI AscI AseI
AsnI Asp718I AspI AviII BbeI
BclI BfrI BsaHI BseAI BsiWI
BspDI BspEI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraIII
DrdI EcoO65I EcoRI EcoRV FbaI
FseI FspI Hin1I HpaI KasI
KpnI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SfoI
SfuI SgrAI SpeI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AflIII 3842 ACACGT A^C[GA][CT]GT
AgeI 776 ACCGGT A^CCGGT
AhdI 1138 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 266 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3273 AGCGCT AGC^GCT
Asp700I 817 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1138 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 2479 GGCACC G^G[CT][GA]CC
BbrPI 3843 CACGTG CAC^GTG
BcgI 2003 CGATTGACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1955 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1955 GCTTAGC GC^T[ACGT]AGC
BsaI 1414 GGTCTC GGTCTC(1/5)
BsaWI 776 ACCGGT [AT]^CCGG[AT]
BsiEI 1999 CGGCCG CG[GA][CT]^CG
BsmBI 1851 GAGACG CGTCTC(1/5)
Bsp1407I 2792 TGTACA T^GTACA
BsrBI 404 GAGCGG CCGCTC(-3/-3)
BsrFI 776 ACCGGT [GA]^CCGG[CT]
BsrGI 2792 TGTACA T^GTACA
Bsu36I 266 CCTCAGG CC^T[ACGT]AGG
CelII 1955 GCTTAGC GC^T[ACGT]AGC
Cfr10I 776 ACCGGT [GA]^CCGG[CT]
EagI 1999 CGGCCG C^GGCCG
Eam1105I 1138 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1999 CGGCCG C^GGCCG
Eco47III 3273 AGCGCT AGC^GCT
Eco52I 1999 CGGCCG C^GGCCG
Eco81I 266 CCTCAGG CC^T[ACGT]AGG
HaeII 3273 AGCGCT [GA]GCGC^[CT]
HgaI 272 GACGC GACGC(5/10)
HincII 131 GTCAAC GT[CT]^[GA]AC
HindII 131 GTCAAC GT[CT]^[GA]AC
HindIII 3347 AAGCTT A^AGCTT
KspI 230 CCGCGG CCGC^GG
PinAI 776 ACCGGT A^CCGGT
PmaCI 3843 CACGTG CAC^GTG
PmeI 2641 GTTTAAAC GTTT^AAAC
PmlI 3843 CACGTG CAC^GTG
SacII 230 CCGCGG CCGC^GG
SbfI 4044 CCTGCAGG CCTGCA^GG
ScaI 1785 AGTACT AGT^ACT
SfiI 399 GGCCCGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 704 CCCGGG CCC^GGG
SnaBI 2776 TACGTA TAC^GTA
SphI 2560 GCATGC GCATG^C
Sse8387I 4044 CCTGCAGG CCTGCA^GG
SspBI 2792 TGTACA T^GTACA
XmaCI 704 CCCGGG C^CCGGG
XmaI 704 CCCGGG C^CCGGG
XmnI 817 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2779 GTAGAC GT^[AC][GT]AC
3264 GTCTAC
AccII 231 CGCG CG^CG
2602 CGCG
BalI 606 TGGCCA TGG^CCA
3938 TGGCCA
BamHI 1898 GGATCC G^GATCC
3810 GGATCC
BciVI 2773 GGATAC GTATCC(6/5)
3161 GGATAC
BglII 3634 AGATCT A^GATCT
4171 AGATCT
BsaAI 2776 TACGTA [CT]AC^GT[GA]
3843 CACGTG
BsaBI 728 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3001 GATGCTCATC
BsgI 374 GTGCAG GTGCAG(16/14)
1248 GTGCAG
BsmI 1388 GAATGC GAATGC(1/-1)
3257 GAATGC
BspHI 204 TCATGA T^CATGA
1736 TCATGA
BstAPI 444 GCATCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3524 GCAGCACCTGC
BstUI 231 CGCG CG^CG
2602 CGCG
MamI 728 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3001 GATGCTCATC
MluNI 606 TGGCCA TGG^CCA
3938 TGGCCA
MscI 606 TGGCCA TGG^CCA
3938 TGGCCA
MslI 1697 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4286 CATGTCTGTG
MvnI 231 CGCG CG^CG
2602 CGCG
NcoI 549 CCATGG C^CATGG
1637 CCATGG
PflMI 1452 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3113 CCAGACCTTGG
PvuII 23 CAGCTG CAG^CTG
3681 CAGCTG
RcaI 204 TCATGA T^CATGA
1736 TCATGA
SacI 1428 GAGCTC GAGCT^C
3604 GAGCTC
SexAI 144 ACCTGGT A^CC[AT]GGT
2283 ACCTGGT
Van91I 1452 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3113 CCAGACCTTGG
XcmI 2407 CCACCTTGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2887 CCATCTATTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII AvrII BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII