##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccI AclI AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
BclI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI KspI MamI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SfiI SfuI SgrAI SpeI
SspBI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 601 GACGTC GACGT^C
Acc65I 1274 GGTACC G^GTACC
AccII 899 CGCG CG^CG
AccIII 1304 TCCGGA T^CCGGA
AcsI 3552 GAATTC [GA]^AATT[CT]
Alw44I 1816 GTGCAC G^TGCAC
ApaLI 1816 GTGCAC G^TGCAC
ApoI 3552 GAATTC [GA]^AATT[CT]
Asp718I 1274 GGTACC G^GTACC
AspI 1573 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2905 TGCGCA TGC^GCA
BamHI 834 GGATCC G^GATCC
BbeI 3015 GGCGCC GGCGC^C
BbrPI 2892 CACGTG CAC^GTG
BcgI 1680 GCAGTGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1629 GGATAC GTATCC(6/5)
BglII 2061 AGATCT A^GATCT
BseAI 1304 TCCGGA T^CCGGA
BsiEI 3844 CGGCCG CG[GA][CT]^CG
BspEI 1304 TCCGGA T^CCGGA
BspLU11I 3472 ACATGT A^CATGT
BsrBI 2325 GAGCGG CCGCTC(-3/-3)
BstUI 899 CGCG CG^CG
DraIII 1312 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2024 GACTGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3844 CGGCCG C^GGCCG
EclXI 3844 CGGCCG C^GGCCG
Eco52I 3844 CGGCCG C^GGCCG
EcoRI 3552 GAATTC G^AATTC
FspI 2905 TGCGCA TGC^GCA
HincII 2359 GTTAAC GT[CT]^[GA]AC
HindII 2359 GTTAAC GT[CT]^[GA]AC
HindIII 935 AAGCTT A^AGCTT
HpaI 2359 GTTAAC GTT^AAC
KasI 3015 GGCGCC G^GCGCC
KpnI 1274 GGTACC GGTAC^C
MroI 1304 TCCGGA T^CCGGA
MvnI 899 CGCG CG^CG
NarI 3015 GGCGCC GG^CGCC
NcoI 554 CCATGG C^CATGG
NdeI 1042 CATATG CA^TATG
PflFI 1573 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2892 CACGTG CAC^GTG
PmlI 2892 CACGTG CAC^GTG
PpuMI 144 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1062 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 3015 GGCGCC GGC^GCC
SmaI 1811 CCCGGG CCC^GGG
SnaBI 1766 TACGTA TAC^GTA
SphI 2250 GCATGC GCATG^C
SspI 1398 AATATT AAT^ATT
TaqI 4126 TCGA T^CGA
TfiI 1449 GAATC G^A[AT]TC
Tsp509I 3553 AATT ^AATT
Tth111I 1573 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 4126 TCGA T^CGA
XcmI 966 CCAGCCTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1811 CCCGGG C^CCGGG
XmaI 1811 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AflII 414 CTTAAG C^TTAAG
626 CTTAAG
AflIII 2378 ACGTGT A^C[GA][CT]GT
3472 ACATGT
AvrII 1365 CCTAGG C^CTAGG
4178 CCTAGG
BfrI 414 CTTAAG C^TTAAG
626 CTTAAG
BlnI 1365 CCTAGG C^CTAGG
4178 CCTAGG
BsaWI 1304 TCCGGA [AT]^CCGG[AT]
1799 ACCGGA
BsrFI 3208 ACCGGC [GA]^CCGG[CT]
3846 GCCGGT
Cfr10I 3208 ACCGGC [GA]^CCGG[CT]
3846 GCCGGT
HgaI 4196 GACGC GACGC(5/10)
610 GCGTC
PleI 893 GACTC GAGTC(4/5)
2330 GAGTC
SapI 390 GAAGAGC GCTCTTC(1/4)
2741 GCTCTTC
SexAI 1297 ACCTGGT A^CC[AT]GGT
3659 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PstI PvuII RsaI
SacI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmlI Sse8387I StuI
StyI Tru9I TseI Tsp45I TspRI
Van91I XhoII