##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BbrPI BbvCI BfrI
BlnI BseAI BsiWI BspEI BspLU11I
Bst1107I BstBI BstZ17I Bsu36I CpoI
DraI EagI EclXI Eco52I Eco81I
EcoRV FseI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsrII
SacII SalI SbfI SexAI SfiI
SfuI SnaBI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3753 GACGTC GACGT^C
Aor51HI 1468 AGCGCT AGC^GCT
BbeI 1020 GGCGCC GGCGC^C
BciVI 218 GGATAC GTATCC(6/5)
BlpI 1976 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1976 GCTCAGC GC^T[ACGT]AGC
BsaAI 403 TACGTG [CT]AC^GT[GA]
BsaI 237 GAGACC GGTCTC(1/5)
BsaWI 2471 ACCGGA [AT]^CCGG[AT]
BsgI 252 GTGCAG GTGCAG(16/14)
BsiEI 1395 CGATCG CG[GA][CT]^CG
BsmI 879 GAATGC GAATGC(1/-1)
BspDI 636 ATCGAT AT^CGAT
BsrBI 89 GAGCGG CCGCTC(-3/-3)
BssHII 710 GCGCGC G^CGCGC
BssSI 4377 CTCGTG CACGAG(-5/-1)
BstEII 2690 GGTTACC G^GT[ACGT]ACC
BstPI 2690 GGTTACC G^GT[ACGT]ACC
CelII 1976 GCTCAGC GC^T[ACGT]AGC
ClaI 636 ATCGAT AT^CGAT
DrdI 1305 GACAGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1468 AGCGCT AGC^GCT
EcoO65I 2690 GGTTACC G^GT[ACGT]ACC
EcoT22I 881 ATGCAT ATGCA^T
KasI 1020 GGCGCC G^GCGCC
NarI 1020 GGCGCC GG^CGCC
NsiI 881 ATGCAT ATGCA^T
NspI 1210 ACATGC [GA]CATG^[CT]
PvuI 1395 CGATCG CGAT^CG
SacI 4002 GAGCTC GAGCT^C
SapI 86 GAAGAGC GCTCTTC(1/4)
SfoI 1020 GGCGCC GGC^GCC
SgrAI 1022 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 3854 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 1020 GGCGCC G[GA]^CG[CT]C
3753 GACGTC
AhdI 1425 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2475 GACTGGCTGTC
AspEI 1425 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2475 GACTGGCTGTC
BcgI 398 CGAGGTACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2909 GCAGGGCCCTCG
BclI 613 TGATCA T^GATCA
3895 TGATCA
BsaBI 1748 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3324 GATGGAGATC
BsaHI 1020 GGCGCC G[GA]^CG[CT]C
3753 GACGTC
BsmBI 1188 GAGACG CGTCTC(1/5)
3666 GAGACG
Eam1105I 1425 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2475 GACTGGCTGTC
EcoRI 245 GAATTC G^AATTC
2571 GAATTC
FbaI 613 TGATCA T^GATCA
3895 TGATCA
HgaI 1370 GACGC GACGC(5/10)
2108 GACGC
Hin1I 1020 GGCGCC G[GA]^CG[CT]C
3753 GACGTC
MamI 1748 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3324 GATGGAGATC
MseI 4155 TTAA T^TAA
4532 TTAA
NcoI 199 CCATGG C^CATGG
3257 CCATGG
SmaI 425 CCCGGG CCC^GGG
554 CCCGGG
Tru9I 4155 TTAA T^TAA
4532 TTAA
XmaCI 425 CCCGGG C^CCGGG
554 CCCGGG
XmaI 425 CCCGGG C^CCGGG
554 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII AviII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII