##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
AscI AseI AsnI AspEI AvrII
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI Eam1105I EcoRI EcoRV HpaI
MamI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PinAI PmeI PshBI PvuI RsrII
SalI SexAI SfuI SgrAI SnaBI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 847 GTCTAC GT^[AC][GT]AC
AclI 3836 AACGTT AA^CGTT
AviII 958 TGCGCA TGC^GCA
BamHI 2629 GGATCC G^GATCC
BbeI 1803 GGCGCC GGCGC^C
BbrPI 3863 CACGTG CAC^GTG
BbvCI 2592 CCTCAGC CCTCAGC(-5/-2)
BcgI 1702 GCAGCTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1846 GGATAC GTATCC(6/5)
BsmBI 589 CGTCTC CGTCTC(1/5)
Bsp1407I 4288 TGTACA T^GTACA
BspHI 4156 TCATGA T^CATGA
BspLU11I 4233 ACATGT A^CATGT
BsrBI 3 CCGCTC CCGCTC(-3/-3)
BsrGI 4288 TGTACA T^GTACA
BssHII 3800 GCGCGC G^CGCGC
BstEII 3466 GGTTACC G^GT[ACGT]ACC
BstPI 3466 GGTTACC G^GT[ACGT]ACC
DrdI 4590 GACACAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3466 GGTTACC G^GT[ACGT]ACC
EcoT22I 4100 ATGCAT ATGCA^T
FseI 1063 GGCCGGCC GGCCGG^CC
FspI 958 TGCGCA TGC^GCA
HincII 2799 GTCAAC GT[CT]^[GA]AC
HindII 2799 GTCAAC GT[CT]^[GA]AC
HindIII 1248 AAGCTT A^AGCTT
KasI 1803 GGCGCC G^GCGCC
NarI 1803 GGCGCC GG^CGCC
NdeI 2551 CATATG CA^TATG
NsiI 4100 ATGCAT ATGCA^T
PaeR7I 555 CTCGAG C^TCGAG
PmaCI 3863 CACGTG CAC^GTG
PmlI 3863 CACGTG CAC^GTG
PshAI 599 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3836 AACGTT AA^CGTT
RcaI 4156 TCATGA T^CATGA
SapI 4178 GAAGAGC GCTCTTC(1/4)
SbfI 654 CCTGCAGG CCTGCA^GG
ScaI 2606 AGTACT AGT^ACT
SfoI 1803 GGCGCC GGC^GCC
SmaI 1697 CCCGGG CCC^GGG
SphI 3927 GCATGC GCATG^C
Sse8387I 654 CCTGCAGG CCTGCA^GG
SspBI 4288 TGTACA T^GTACA
XbaI 2704 TCTAGA T^CTAGA
XcmI 1948 CCACCCCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 555 CTCGAG C^TCGAG
XmaCI 1697 CCCGGG C^CCGGG
XmaI 1697 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1555 GGTACC G^GTACC
3807 GGTACC
AflIII 475 ACGTGT A^C[GA][CT]GT
4233 ACATGT
Alw44I 1629 GTGCAC G^TGCAC
3704 GTGCAC
Aor51HI 2121 AGCGCT AGC^GCT
2596 AGCGCT
ApaLI 1629 GTGCAC G^TGCAC
3704 GTGCAC
Asp700I 2736 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3533 GAAGTTGTTC
Asp718I 1555 GGTACC G^GTACC
3807 GGTACC
AspI 1617 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3753 GACCAAGTC
BclI 298 TGATCA T^GATCA
3164 TGATCA
BlpI 2219 GCTCAGC GC^T[ACGT]AGC
4508 GCTCAGC
Bpu1102I 2219 GCTCAGC GC^T[ACGT]AGC
4508 GCTCAGC
BsiEI 1062 CGGCCG CG[GA][CT]^CG
1679 CGGCCG
BsmI 269 GCATTC GAATGC(1/-1)
2859 GCATTC
BsrDI 2433 CATTGC GCAATG(2/0)
85 GCAATG
CelII 2219 GCTCAGC GC^T[ACGT]AGC
4508 GCTCAGC
DraIII 1008 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
3863 CACGTGGTG
EagI 1062 CGGCCG C^GGCCG
1679 CGGCCG
EclXI 1062 CGGCCG C^GGCCG
1679 CGGCCG
Eco47III 2121 AGCGCT AGC^GCT
2596 AGCGCT
Eco52I 1062 CGGCCG C^GGCCG
1679 CGGCCG
FbaI 298 TGATCA T^GATCA
3164 TGATCA
KpnI 1555 GGTACC GGTAC^C
3807 GGTACC
KspI 322 CCGCGG CCGC^GG
2159 CCGCGG
NaeI 1064 GCCGGC GCC^GGC
2001 GCCGGC
NgoMIV 1064 GCCGGC G^CCGGC
2001 GCCGGC
NspI 3927 GCATGC [GA]CATG^[CT]
4233 ACATGT
PflFI 1617 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3753 GACCAAGTC
SacI 18 GAGCTC GAGCT^C
4059 GAGCTC
SacII 322 CCGCGG CCGC^GG
2159 CCGCGG
SfiI 119 GGCCAGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1680 GGCCGCACAGGCC
StuI 1688 AGGCCT AGG^CCT
2013 AGGCCT
Tth111I 1617 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3753 GACCAAGTC
XmnI 2736 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3533 GAAGTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII