##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AgeI AhdI Alw44I ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BcgI BlnI BsaBI BsiEI
BsmBI Bsp1407I BspDI BsrBI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FseI FspI
HgaI HindIII KpnI KspI MamI
MluI MvnI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 1394 TCCGGA T^CCGGA
AcsI 1732 GAATTT [GA]^AATT[CT]
AcyI 2502 GGCGCC G[GA]^CG[CT]C
AflII 1011 CTTAAG C^TTAAG
Aor51HI 767 AGCGCT AGC^GCT
ApoI 1732 GAATTT [GA]^AATT[CT]
AseI 3601 ATTAAT AT^TAAT
AsnI 3601 ATTAAT AT^TAAT
BbeI 2502 GGCGCC GGCGC^C
BfrI 1011 CTTAAG C^TTAAG
BglII 3590 AGATCT A^GATCT
BsaHI 2502 GGCGCC G[GA]^CG[CT]C
BsaI 1702 GAGACC GGTCTC(1/5)
BseAI 1394 TCCGGA T^CCGGA
BsiWI 3399 CGTACG C^GTACG
BsmI 1952 GCATTC GAATGC(1/-1)
BspEI 1394 TCCGGA T^CCGGA
BspHI 3381 TCATGA T^CATGA
BssSI 2809 CACGAG CACGAG(-5/-1)
BstEII 466 GGTCACC G^GT[ACGT]ACC
BstPI 466 GGTCACC G^GT[ACGT]ACC
DraI 3608 TTTAAA TTT^AAA
DraIII 398 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 969 GACGGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 767 AGCGCT AGC^GCT
EcoO65I 466 GGTCACC G^GT[ACGT]ACC
EcoT22I 3389 ATGCAT ATGCA^T
Hin1I 2502 GGCGCC G[GA]^CG[CT]C
HincII 1870 GTTAAC GT[CT]^[GA]AC
HindII 1870 GTTAAC GT[CT]^[GA]AC
HpaI 1870 GTTAAC GTT^AAC
KasI 2502 GGCGCC G^GCGCC
MroI 1394 TCCGGA T^CCGGA
NaeI 2904 GCCGGC GCC^GGC
NarI 2502 GGCGCC GG^CGCC
NgoMIV 2904 GCCGGC G^CCGGC
NsiI 3389 ATGCAT ATGCA^T
PshBI 3601 ATTAAT AT^TAAT
RcaI 3381 TCATGA T^CATGA
SexAI 2754 ACCTGGT A^CC[AT]GGT
SfoI 2502 GGCGCC GGC^GCC
SmaI 855 CCCGGG CCC^GGG
XmaCI 855 CCCGGG C^CCGGG
XmaI 855 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BamHI 2542 GGATCC G^GATCC
2657 GGATCC
BciVI 3247 GGATAC GTATCC(6/5)
3501 GGATAC
BclI 3345 TGATCA T^GATCA
4085 TGATCA
BsaWI 1270 TCCGGT [AT]^CCGG[AT]
1394 TCCGGA
BsrDI 491 GCAATG GCAATG(2/0)
2529 GCAATG
BsrFI 1486 ACCGGC [GA]^CCGG[CT]
2904 GCCGGC
BstAPI 124 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
198 GCATTGTGTGC
Cfr10I 1486 ACCGGC [GA]^CCGG[CT]
2904 GCCGGC
DsaI 1024 CCACGG C^C[GA][CT]GG
1618 CCGTGG
FbaI 3345 TGATCA T^GATCA
4085 TGATCA
MfeI 874 CAATTG C^AATTG
3349 CAATTG
MslI 3157 CACCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3382 CATGACAATG
MunI 874 CAATTG C^AATTG
3349 CAATTG
PshAI 2183 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3534 GACAAATGTC
StuI 1361 AGGCCT AGG^CCT
1667 AGGCCT
TaqI 479 TCGA T^CGA
4072 TCGA
TthHB8I 479 TCGA T^CGA
4072 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII