##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
Asp700I Asp718I AspEI BbvCI BclI
BfrI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspHI
Bst1107I BstAPI BstZ17I CelII ClaI
CpoI DraI Eam1105I EcoRV EcoT22I
FbaI HpaI KpnI MamI MfeI
MluI MroI MunI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
Psp1406I PvuI RcaI RsrII SalI
SexAI SfiI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI SwaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1606 GTAGAC GT^[AC][GT]AC
Aor51HI 555 AGCGCT AGC^GCT
AseI 1593 ATTAAT AT^TAAT
AsnI 1593 ATTAAT AT^TAAT
AspI 1803 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 360 CCCGAG C^[CT]CG[GA]G
AviII 754 TGCGCA TGC^GCA
BamHI 1036 GGATCC G^GATCC
BciVI 1343 GGATAC GTATCC(6/5)
BsaWI 1529 TCCGGT [AT]^CCGG[AT]
BsiEI 601 CGGCCG CG[GA][CT]^CG
BsmI 1907 GCATTC GAATGC(1/-1)
BsoBI 360 CCCGAG C^[CT]CG[GA]G
BspLU11I 1524 ACATGT A^CATGT
BssSI 3634 CACGAG CACGAG(-5/-1)
BstEII 3824 GGTAACC G^GT[ACGT]ACC
BstPI 3824 GGTAACC G^GT[ACGT]ACC
EagI 601 CGGCCG C^GGCCG
EclXI 601 CGGCCG C^GGCCG
Eco47III 555 AGCGCT AGC^GCT
Eco52I 601 CGGCCG C^GGCCG
EcoO65I 3824 GGTAACC G^GT[ACGT]ACC
EcoRI 2448 GAATTC G^AATTC
FseI 746 GGCCGGCC GGCCGG^CC
FspI 754 TGCGCA TGC^GCA
HgaI 3731 GCGTC GACGC(5/10)
HindIII 3289 AAGCTT A^AGCTT
KspI 334 CCGCGG CCGC^GG
NdeI 2621 CATATG CA^TATG
NheI 2277 GCTAGC G^CTAGC
NspI 1524 ACATGT [GA]CATG^[CT]
PflFI 1803 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3740 CCACGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1134 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1593 ATTAAT AT^TAAT
SacII 334 CCGCGG CCGC^GG
SbfI 2604 CCTGCAGG CCTGCA^GG
ScaI 3615 AGTACT AGT^ACT
Sse8387I 2604 CCTGCAGG CCTGCA^GG
Tth111I 1803 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3740 CCACGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 2448 GAATTC [GA]^AATT[CT]
4103 AAATTC
AcyI 385 GGCGCC G[GA]^CG[CT]C
839 GGCGCC
AlwNI 2248 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2608 CAGGTGCTG
AocI 3812 CCTCAGG CC^T[ACGT]AGG
4365 CCTGAGG
ApoI 2448 GAATTC [GA]^AATT[CT]
4103 AAATTC
BbeI 385 GGCGCC GGCGC^C
839 GGCGCC
BbsI 3136 GTCTTC GAAGAC(2/6)
3505 GTCTTC
BcgI 291 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4209 GCAGAAAGTTCG
BglI 3411 GCCCATAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4324 GCCCTCCTGGC
BpuAI 3136 GTCTTC GAAGAC(2/6)
3505 GTCTTC
BsaHI 385 GGCGCC G[GA]^CG[CT]C
839 GGCGCC
BsgI 487 CTGCAC GTGCAG(16/14)
798 CTGCAC
Bsp1407I 1383 TGTACA T^GTACA
1699 TGTACA
BsrGI 1383 TGTACA T^GTACA
1699 TGTACA
BstBI 967 TTCGAA TT^CGAA
4343 TTCGAA
Bsu36I 3812 CCTCAGG CC^T[ACGT]AGG
4365 CCTGAGG
DrdI 3172 GACCTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4160 GACCCTCCTGTC
Eco81I 3812 CCTCAGG CC^T[ACGT]AGG
4365 CCTGAGG
EcoNI 1411 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4374 CCTGAGAGAGG
Hin1I 385 GGCGCC G[GA]^CG[CT]C
839 GGCGCC
HincII 2222 GTCAAC GT[CT]^[GA]AC
2300 GTTGAC
HindII 2222 GTCAAC GT[CT]^[GA]AC
2300 GTTGAC
KasI 385 GGCGCC G^GCGCC
839 GGCGCC
NaeI 747 GCCGGC GCC^GGC
3844 GCCGGC
NarI 385 GGCGCC GG^CGCC
839 GGCGCC
NgoMIV 747 GCCGGC G^CCGGC
3844 GCCGGC
NspV 967 TTCGAA TT^CGAA
4343 TTCGAA
SfoI 385 GGCGCC GGC^GCC
839 GGCGCC
SfuI 967 TTCGAA TT^CGAA
4343 TTCGAA
SspBI 1383 TGTACA T^GTACA
1699 TGTACA
StuI 1059 AGGCCT AGG^CCT
3638 AGGCCT
XbaI 1195 TCTAGA T^CTAGA
1657 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI ApaI AspHI AvaII AvrII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BglII BlnI BmyI
BpmI BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII