##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AflII AhdI AscI AseI
AsnI AspEI BbrPI BcgI BciVI
BclI BfrI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI MamI MfeI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SalI
ScaI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 865 GGTACC G^GTACC
AflIII 116 ACGCGT A^C[GA][CT]GT
Aor51HI 1250 AGCGCT AGC^GCT
Asp700I 87 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 865 GGTACC G^GTACC
AvrII 944 CCTAGG C^CTAGG
BamHI 286 GGATCC G^GATCC
BbeI 721 GGCGCC GGCGC^C
BglII 141 AGATCT A^GATCT
BlnI 944 CCTAGG C^CTAGG
BsaAI 1815 TACGTG [CT]AC^GT[GA]
BssSI 701 CTCGTG CACGAG(-5/-1)
BstXI 1754 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 163 CGGACCG CG^G[AT]CCG
DraI 3279 TTTAAA TTT^AAA
DraIII 230 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 512 GACGGGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1055 CGGCCG C^GGCCG
EclXI 1055 CGGCCG C^GGCCG
Eco47III 1250 AGCGCT AGC^GCT
Eco52I 1055 CGGCCG C^GGCCG
HincII 3445 GTTGAC GT[CT]^[GA]AC
HindII 3445 GTTGAC GT[CT]^[GA]AC
KasI 721 GGCGCC G^GCGCC
KpnI 865 GGTACC GGTAC^C
MluI 116 ACGCGT A^CGCGT
NaeI 1731 GCCGGC GCC^GGC
NarI 721 GGCGCC GG^CGCC
NgoMIV 1731 GCCGGC G^CCGGC
PaeR7I 1967 CTCGAG C^TCGAG
PshAI 876 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 163 CGGACCG CG^G[AT]CCG
SacI 1992 GAGCTC GAGCT^C
SapI 34 GCTCTTC GCTCTTC(1/4)
SexAI 1241 ACCTGGT A^CC[AT]GGT
SfiI 1096 GGCCCGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 721 GGCGCC GGC^GCC
SphI 185 GCATGC GCATG^C
StuI 2294 AGGCCT AGG^CCT
XhoI 1967 CTCGAG C^TCGAG
XmnI 87 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 518 GACGTC GACGT^C
2358 GACGTC
AccIII 834 TCCGGA T^CCGGA
2150 TCCGGA
AcsI 268 AAATTC [GA]^AATT[CT]
3499 AAATTC
AfaI 160 GTAC GT^AC
866 GTAC
Alw44I 1280 GTGCAC G^TGCAC
2802 GTGCAC
ApaLI 1280 GTGCAC G^TGCAC
2802 GTGCAC
ApoI 268 AAATTC [GA]^AATT[CT]
3499 AAATTC
AviII 188 TGCGCA TGC^GCA
388 TGCGCA
BbsI 78 GTCTTC GAAGAC(2/6)
3617 GTCTTC
BlpI 2021 GCTGAGC GC^T[ACGT]AGC
3641 GCTCAGC
Bpu1102I 2021 GCTGAGC GC^T[ACGT]AGC
3641 GCTCAGC
BpuAI 78 GTCTTC GAAGAC(2/6)
3617 GTCTTC
BseAI 834 TCCGGA T^CCGGA
2150 TCCGGA
BsiEI 1055 CGGCCG CG[GA][CT]^CG
1089 CGACCG
BspEI 834 TCCGGA T^CCGGA
2150 TCCGGA
BstAPI 3045 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3748 GCAGGGGCTGC
BstYI 141 AGATCT [GA]^GATC[CT]
286 GGATCC
CelII 2021 GCTGAGC GC^T[ACGT]AGC
3641 GCTCAGC
EcoNI 1116 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1741 CCTGAAATAGG
FspI 188 TGCGCA TGC^GCA
388 TGCGCA
MflI 141 AGATCT [GA]^GATC[CT]
286 GGATCC
MroI 834 TCCGGA T^CCGGA
2150 TCCGGA
NcoI 600 CCATGG C^CATGG
1808 CCATGG
NspI 185 GCATGC [GA]CATG^[CT]
1234 GCATGT
PpuMI 939 GGGTCCC [GA]G^G[AT]CC[CT]
1654 GGGACCC
RsaI 160 GTAC GT^AC
866 GTAC
SbfI 319 CCTGCAGG CCTGCA^GG
2078 CCTGCAGG
Sse8387I 319 CCTGCAGG CCTGCA^GG
2078 CCTGCAGG
XhoII 141 AGATCT [GA]^GATC[CT]
286 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcyI AgeI
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflFI PflMI PinAI PleI
PstI PvuII SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XmaCI XmaI