##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI BcgI BciVI BfrI
BglII BlpI Bpu1102I BseAI BsiEI
BsiWI BsmI BspDI BspEI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 788 GGTACC G^GTACC
AccI 2406 GTAGAC GT^[AC][GT]AC
AcsI 2673 GAATTT [GA]^AATT[CT]
AflIII 3138 ACATGT A^C[GA][CT]GT
Alw44I 1272 GTGCAC G^TGCAC
ApaLI 1272 GTGCAC G^TGCAC
ApoI 2673 GAATTT [GA]^AATT[CT]
Asp718I 788 GGTACC G^GTACC
AvrII 1190 CCTAGG C^CTAGG
BamHI 1012 GGATCC G^GATCC
BclI 1443 TGATCA T^GATCA
BlnI 1190 CCTAGG C^CTAGG
BsaWI 117 ACCGGA [AT]^CCGG[AT]
BspLU11I 3138 ACATGT A^CATGT
BsrDI 4404 GCAATG GCAATG(2/0)
BstAPI 16 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 3438 GACCTCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1443 TGATCA T^GATCA
KpnI 788 GGTACC GGTAC^C
KspI 1968 CCGCGG CCGC^GG
MslI 1265 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 82 GCCGGC GCC^GGC
NcoI 2447 CCATGG C^CATGG
NgoMIV 82 GCCGGC G^CCGGC
SacII 1968 CCGCGG CCGC^GG
SapI 2832 GCTCTTC GCTCTTC(1/4)
ScaI 2277 AGTACT AGT^ACT
XbaI 1118 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 1419 GGGCCC GGGCC^C
4310 GGGCCC
AspI 897 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1097 GACCGGGTC
AviII 2025 TGCGCA TGC^GCA
3055 TGCGCA
BbrPI 1151 CACGTG CAC^GTG
3477 CACGTG
BbsI 266 GAAGAC GAAGAC(2/6)
2771 GAAGAC
BpuAI 266 GAAGAC GAAGAC(2/6)
2771 GAAGAC
BsaAI 1151 CACGTG [CT]AC^GT[GA]
3477 CACGTG
BsaBI 2940 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4119 GATCGACATC
BsgI 1586 GTGCAG GTGCAG(16/14)
3153 GTGCAG
BsmBI 311 GAGACG CGTCTC(1/5)
4103 GAGACG
Bsp1407I 3135 TGTACA T^GTACA
4432 TGTACA
BspHI 72 TCATGA T^CATGA
1571 TCATGA
BsrGI 3135 TGTACA T^GTACA
4432 TGTACA
BssHII 3827 GCGCGC G^CGCGC
3829 GCGCGC
DraI 4352 TTTAAA TTT^AAA
4418 TTTAAA
FspI 2025 TGCGCA TGC^GCA
3055 TGCGCA
MamI 2940 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4119 GATCGACATC
PflFI 897 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1097 GACCGGGTC
PmaCI 1151 CACGTG CAC^GTG
3477 CACGTG
PmlI 1151 CACGTG CAC^GTG
3477 CACGTG
RcaI 72 TCATGA T^CATGA
1571 TCATGA
SspBI 3135 TGTACA T^GTACA
4432 TGTACA
TaqI 1225 TCGA T^CGA
4121 TCGA
Tth111I 897 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1097 GACCGGGTC
TthHB8I 1225 TCGA T^CGA
4121 TCGA
XcmI 2591 CCAGAGCAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3386 CCAGGGCCAGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmaCI XmaI