##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AvrII BanI BbeI
BciVI BclI BfrI BglI BlnI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
BssSI BstBI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FbaI FseI HaeII HindIII
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SspBI StuI SwaI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 4154 AACGTT AA^CGTT
AcyI 3853 GGCGTC G[GA]^CG[CT]C
AflIII 2745 ACATGT A^C[GA][CT]GT
AspI 2063 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 83 TGCGCA TGC^GCA
BamHI 915 GGATCC G^GATCC
BbrPI 1530 CACGTG CAC^GTG
BcgI 4121 CGAGTTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 877 AGATCT A^GATCT
BlpI 1303 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1303 GCTTAGC GC^T[ACGT]AGC
BsaHI 3853 GGCGTC G[GA]^CG[CT]C
BsiEI 424 CGACCG CG[GA][CT]^CG
BspLU11I 2745 ACATGT A^CATGT
BsrDI 3870 CATTGC GCAATG(2/0)
BstEII 2219 GGTTACC G^GT[ACGT]ACC
BstPI 2219 GGTTACC G^GT[ACGT]ACC
CelII 1303 GCTTAGC GC^T[ACGT]AGC
EcoO65I 2219 GGTTACC G^GT[ACGT]ACC
EcoRI 3801 GAATTC G^AATTC
EcoRV 3345 GATATC GAT^ATC
FauI 2937 GCGGG CCCGC(4/6)
FspI 83 TGCGCA TGC^GCA
HgaI 3854 GCGTC GACGC(5/10)
Hin1I 3853 GGCGTC G[GA]^CG[CT]C
MfeI 2327 CAATTG C^AATTG
MunI 2327 CAATTG C^AATTG
NcoI 3979 CCATGG C^CATGG
NdeI 2008 CATATG CA^TATG
PflFI 2063 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1530 CACGTG CAC^GTG
PmlI 1530 CACGTG CAC^GTG
PshAI 1505 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4154 AACGTT AA^CGTT
SacI 754 GAGCTC GAGCT^C
ScaI 476 AGTACT AGT^ACT
SmaI 3080 CCCGGG CCC^GGG
SnaBI 2802 TACGTA TAC^GTA
SspI 2658 AATATT AAT^ATT
Tsp45I 3745 GTGAC ^GT[GC]AC
Tth111I 2063 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3080 CCCGGG C^CCGGG
XmaI 3080 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 3027 CGCG CG^CG
3112 CGCG
AlwNI 1713 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1798 CAGCCCCTG
ApaI 328 GGGCCC GGGCC^C
1484 GGGCCC
AseI 1099 ATTAAT AT^TAAT
3490 ATTAAT
AsnI 1099 ATTAAT AT^TAAT
3490 ATTAAT
AspHI 754 GAGCTC G[AT]GC[AT]^C
2410 GAGCAC
AvaI 3047 CTCGGG C^[CT]CG[GA]G
3080 CCCGGG
BalI 284 TGGCCA TGG^CCA
2423 TGGCCA
BsaAI 1530 CACGTG [CT]AC^GT[GA]
2802 TACGTA
BsaBI 885 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1765 GATTGTGATC
BseRI 199 CTCCTC GAGGAG(10/8)
4005 GAGGAG
BsgI 3119 CTGCAC GTGCAG(16/14)
552 GTGCAG
BsiHKAI 754 GAGCTC G[AT]GC[AT]^C
2410 GAGCAC
BsmI 593 GAATGC GAATGC(1/-1)
4127 GAATGC
BsoBI 3047 CTCGGG C^[CT]CG[GA]G
3080 CCCGGG
BsrFI 62 ACCGGC [GA]^CCGG[CT]
1584 GCCGGT
BstAPI 495 GCATGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3088 GCAGAGGTTGC
BstUI 3027 CGCG CG^CG
3112 CGCG
Cfr10I 62 ACCGGC [GA]^CCGG[CT]
1584 GCCGGT
HincII 2448 GTTAAC GT[CT]^[GA]AC
4111 GTTAAC
HindII 2448 GTTAAC GT[CT]^[GA]AC
4111 GTTAAC
HpaI 2448 GTTAAC GTT^AAC
4111 GTTAAC
MamI 885 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1765 GATTGTGATC
MluNI 284 TGGCCA TGG^CCA
2423 TGGCCA
MscI 284 TGGCCA TGG^CCA
2423 TGGCCA
MslI 653 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2622 CACAGAAATG
MvnI 3027 CGCG CG^CG
3112 CGCG
NspI 2745 ACATGT [GA]CATG^[CT]
3428 GCATGT
PshBI 1099 ATTAAT AT^TAAT
3490 ATTAAT
SapI 42 GAAGAGC GCTCTTC(1/4)
607 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI Asp700I AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
Bst1107I BstF5I BstNI BstXI BstYI
BstZ17I Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XcmI XhoII XmnI