##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII BbrPI BbsI BcgI
BciVI BfrI BpuAI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
AatII 2986 GACGTC GACGT^C
BclI 2453 TGATCA T^GATCA
BglII 2306 AGATCT A^GATCT
BlpI 1531 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1531 GCTGAGC GC^T[ACGT]AGC
BsaAI 2150 TACGTG [CT]AC^GT[GA]
BsmBI 3202 CGTCTC CGTCTC(1/5)
BsrFI 2602 GCCGGC [GA]^CCGG[CT]
CelII 1531 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2602 GCCGGC [GA]^CCGG[CT]
DraI 2793 TTTAAA TTT^AAA
DraIII 2418 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 511 GACACAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2453 TGATCA T^GATCA
NaeI 2602 GCCGGC GCC^GGC
NcoI 456 CCATGG C^CATGG
NgoMIV 2602 GCCGGC G^CCGGC
PshAI 3578 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3825 CGATCG CGAT^CG
SapI 3777 GCTCTTC GCTCTTC(1/4)
SbfI 3870 CCTGCAGG CCTGCA^GG
Sse8387I 3870 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 1502 GGTACC G^GTACC
1892 GGTACC
Asp718I 1502 GGTACC G^GTACC
1892 GGTACC
AvrII 1673 CCTAGG C^CTAGG
2432 CCTAGG
BamHI 62 GGATCC G^GATCC
2165 GGATCC
BlnI 1673 CCTAGG C^CTAGG
2432 CCTAGG
BsaI 2061 GAGACC GGTCTC(1/5)
4008 GAGACC
BsrDI 4289 CATTGC GCAATG(2/0)
3559 GCAATG
DsaI 456 CCATGG C^C[GA][CT]GG
776 CCACGG
EagI 3232 CGGCCG C^GGCCG
3324 CGGCCG
EclXI 3232 CGGCCG C^GGCCG
3324 CGGCCG
Eco52I 3232 CGGCCG C^GGCCG
3324 CGGCCG
HgaI 1177 GCGTC GACGC(5/10)
3201 GCGTC
KpnI 1502 GGTACC GGTAC^C
1892 GGTACC
MaeII 2151 ACGT A^CGT
2987 ACGT
NspI 1918 GCATGC [GA]CATG^[CT]
2967 GCATGC
PaeR7I 380 CTCGAG C^TCGAG
1456 CTCGAG
SmaI 672 CCCGGG CCC^GGG
2700 CCCGGG
SphI 1918 GCATGC GCATG^C
2967 GCATGC
TfiI 413 GATTC G^A[AT]TC
4249 GAATC
XhoI 380 CTCGAG C^TCGAG
1456 CTCGAG
XmaCI 672 CCCGGG C^CCGGG
2700 CCCGGG
XmaI 672 CCCGGG C^CCGGG
2700 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII