##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I ApaLI AscI Asp700I
Asp718I AspI AvrII BamHI BbrPI
BbvCI BciVI BclI BglI BlnI
BsaAI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstXI Cfr10I ClaI CpoI DrdI
EcoO65I EcoRV FbaI FseI HgaI
KpnI KspI MluI MroI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI TaqI Tth111I TthHB8I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1464 GTATAC GT^[AC][GT]AC
AocI 1403 CCTAAGG CC^T[ACGT]AGG
ApaI 4409 GGGCCC GGGCC^C
AseI 3068 ATTAAT AT^TAAT
AsnI 3068 ATTAAT AT^TAAT
AvaI 4474 CTCGGG C^[CT]CG[GA]G
BalI 1374 TGGCCA TGG^CCA
BcnI 48 CCCGG CC^[GC]GG
BlpI 4400 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 4400 GCTAAGC GC^T[ACGT]AGC
BsiEI 13 CGGCCG CG[GA][CT]^CG
BsoBI 4474 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3971 TGTACA T^GTACA
BspMI 388 GCAGGT ACCTGC(4/8)
BsrGI 3971 TGTACA T^GTACA
Bst1107I 1464 GTATAC GTA^TAC
BstAPI 1146 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1464 GTATAC GTA^TAC
Bsu36I 1403 CCTAAGG CC^T[ACGT]AGG
CelII 4400 GCTAAGC GC^T[ACGT]AGC
DsaI 4433 CCATGG C^C[GA][CT]GG
EagI 13 CGGCCG C^GGCCG
EclXI 13 CGGCCG C^GGCCG
Eco52I 13 CGGCCG C^GGCCG
Eco81I 1403 CCTAAGG CC^T[ACGT]AGG
HpaI 2889 GTTAAC GTT^AAC
MfeI 805 CAATTG C^AATTG
MluNI 1374 TGGCCA TGG^CCA
MscI 1374 TGGCCA TGG^CCA
MunI 805 CAATTG C^AATTG
NciI 48 CCCGG CC^[GC]GG
NcoI 4433 CCATGG C^CATGG
NdeI 578 CATATG CA^TATG
PpuMI 4359 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 3431 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3068 ATTAAT AT^TAAT
SacI 4471 GAGCTC GAGCT^C
SexAI 4585 ACCTGGT A^CC[AT]GGT
SpeI 1030 ACTAGT A^CTAGT
SphI 3760 GCATGC GCATG^C
SspBI 3971 TGTACA T^GTACA
XbaI 3000 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 51 GGCGCC G[GA]^CG[CT]C
633 GGCGCC
AhdI 3 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2213 GACATGGAGTC
AlwNI 652 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1147 CAGTCCCTG
Aor51HI 1238 AGCGCT AGC^GCT
1255 AGCGCT
AspEI 3 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2213 GACATGGAGTC
AvaII 1213 GGACC G^G[AT]CC
4360 GGACC
AviII 2870 TGCGCA TGC^GCA
3757 TGCGCA
BanI 51 GGCGCC G^G[CT][GA]CC
633 GGCGCC
BbeI 51 GGCGCC GGCGC^C
633 GGCGCC
BcgI 378 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2719 GCAATAAGTTCG
BglII 1873 AGATCT A^GATCT
3036 AGATCT
BsaHI 51 GGCGCC G[GA]^CG[CT]C
633 GGCGCC
DraII 4359 GGGACCT [GA]G^G[ACGT]CC[CT]
4408 GGGGCCC
DraIII 2491 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4584 CACCTGGTG
EaeI 13 CGGCCG [CT]^GGCC[GA]
1374 TGGCCA
Eam1105I 3 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2213 GACATGGAGTC
Eco47III 1238 AGCGCT AGC^GCT
1255 AGCGCT
EcoNI 1985 CCTACTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2417 CCTCTGTTAGG
EcoO109I 4359 GGGACCT [GA]G^G[ACGT]CC[CT]
4408 GGGGCCC
EcoT14I 2430 CCAAGG C^C[AT][AT]GG
4433 CCATGG
EcoT22I 3336 ATGCAT ATGCA^T
3762 ATGCAT
FauI 2362 GCGGG CCCGC(4/6)
3392 GCGGG
FspI 2870 TGCGCA TGC^GCA
3757 TGCGCA
Hin1I 51 GGCGCC G[GA]^CG[CT]C
633 GGCGCC
HincII 2889 GTTAAC GT[CT]^[GA]AC
4093 GTTGAC
HindII 2889 GTTAAC GT[CT]^[GA]AC
4093 GTTGAC
KasI 51 GGCGCC G^GCGCC
633 GGCGCC
NarI 51 GGCGCC GG^CGCC
633 GGCGCC
NsiI 3336 ATGCAT ATGCA^T
3762 ATGCAT
PvuII 987 CAGCTG CAG^CTG
1343 CAGCTG
SfoI 51 GGCGCC GGC^GCC
633 GGCGCC
SspI 1494 AATATT AAT^ATT
3929 AATATT
StyI 2430 CCAAGG C^C[AT][AT]GG
4433 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI ApoI AspHI BanII
BbsI BbvI BfaI BfrI BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRI
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII