##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII BcgI BciVI BsaBI
BseAI BsgI BsiWI BsmI BspDI
BspEI BspLU11I BsrDI Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoRI EcoT22I FseI FspI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2791 GTAGAC GT^[AC][GT]AC
AflII 430 CTTAAG C^TTAAG
AgeI 1802 ACCGGT A^CCGGT
AvrII 2312 CCTAGG C^CTAGG
BbvCI 4164 CCTCAGC CCTCAGC(-5/-2)
BclI 680 TGATCA T^GATCA
BfrI 430 CTTAAG C^TTAAG
BglII 846 AGATCT A^GATCT
BlnI 2312 CCTAGG C^CTAGG
BlpI 651 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 651 GCTCAGC GC^T[ACGT]AGC
BsiEI 3285 CGGCCG CG[GA][CT]^CG
Bsp1407I 383 TGTACA T^GTACA
BspHI 3443 TCATGA T^CATGA
BsrGI 383 TGTACA T^GTACA
BssHII 101 GCGCGC G^CGCGC
BstEII 438 GGTCACC G^GT[ACGT]ACC
BstPI 438 GGTCACC G^GT[ACGT]ACC
CelII 651 GCTCAGC GC^T[ACGT]AGC
DraI 2063 TTTAAA TTT^AAA
DrdI 2291 GACAGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3285 CGGCCG C^GGCCG
EclXI 3285 CGGCCG C^GGCCG
Eco52I 3285 CGGCCG C^GGCCG
EcoNI 2204 CCTTGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 438 GGTCACC G^GT[ACGT]ACC
EcoRV 1908 GATATC GAT^ATC
FbaI 680 TGATCA T^GATCA
HindIII 3259 AAGCTT A^AGCTT
HpaI 2042 GTTAAC GTT^AAC
PaeR7I 3124 CTCGAG C^TCGAG
PinAI 1802 ACCGGT A^CCGGT
RcaI 3443 TCATGA T^CATGA
SacI 2724 GAGCTC GAGCT^C
SexAI 442 ACCAGGT A^CC[AT]GGT
SfiI 2181 GGCCAGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 383 TGTACA T^GTACA
SspI 4381 AATATT AAT^ATT
XhoI 3124 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1720 GAATTT [GA]^AATT[CT]
2066 AAATTT
AflIII 161 ACGTGT A^C[GA][CT]GT
1023 ACACGT
Alw44I 157 GTGCAC G^TGCAC
3550 GTGCAC
ApaLI 157 GTGCAC G^TGCAC
3550 GTGCAC
ApoI 1720 GAATTT [GA]^AATT[CT]
2066 AAATTT
Asp700I 2075 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2371 GAAGCCTTTC
AspI 199 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
304 GACGGTGTC
BamHI 601 GGATCC G^GATCC
3019 GGATCC
BbeI 244 GGCGCC GGCGC^C
3851 GGCGCC
BbrPI 160 CACGTG CAC^GTG
1024 CACGTG
BsaWI 1127 TCCGGT [AT]^CCGG[AT]
1802 ACCGGT
BssSI 2799 CACGAG CACGAG(-5/-1)
4283 CTCGTG
HincII 493 GTTGAC GT[CT]^[GA]AC
2042 GTTAAC
HindII 493 GTTGAC GT[CT]^[GA]AC
2042 GTTAAC
KasI 244 GGCGCC G^GCGCC
3851 GGCGCC
KspI 506 CCGCGG CCGC^GG
1248 CCGCGG
NaeI 474 GCCGGC GCC^GGC
3403 GCCGGC
NarI 244 GGCGCC GG^CGCC
3851 GGCGCC
NgoMIV 474 GCCGGC G^CCGGC
3403 GCCGGC
NspI 2783 ACATGC [GA]CATG^[CT]
2992 GCATGT
PflFI 199 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
304 GACGGTGTC
PmaCI 160 CACGTG CAC^GTG
1024 CACGTG
PmlI 160 CACGTG CAC^GTG
1024 CACGTG
SacII 506 CCGCGG CCGC^GG
1248 CCGCGG
SfoI 244 GGCGCC GGC^GCC
3851 GGCGCC
TfiI 1065 GAATC G^A[AT]TC
3604 GATTC
Tth111I 199 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
304 GACGGTGTC
XmnI 2075 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2371 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII