##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII BbeI BbrPI BbvCI BcgI
BfrI BglII BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII BstBI ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1148 GACGTC GACGT^C
AccI 3975 GTATAC GT^[AC][GT]AC
AcyI 1148 GACGTC G[GA]^CG[CT]C
AfaI 1403 GTAC GT^AC
AflIII 2268 ACATGT A^C[GA][CT]GT
AvrII 560 CCTAGG C^CTAGG
BciVI 3413 GTATCC GTATCC(6/5)
BglI 488 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 560 CCTAGG C^CTAGG
BlpI 3050 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3050 GCTCAGC GC^T[ACGT]AGC
BsaAI 1779 TACGTG [CT]AC^GT[GA]
BsaHI 1148 GACGTC G[GA]^CG[CT]C
BsaWI 1405 ACCGGA [AT]^CCGG[AT]
BspLU11I 2268 ACATGT A^CATGT
BspMI 1844 ACCTGC ACCTGC(4/8)
BsrFI 1056 ACCGGC [GA]^CCGG[CT]
BssSI 3124 CACGAG CACGAG(-5/-1)
Bst1107I 3975 GTATAC GTA^TAC
BstEII 1880 GGTCACC G^GT[ACGT]ACC
BstPI 1880 GGTCACC G^GT[ACGT]ACC
BstZ17I 3975 GTATAC GTA^TAC
CelII 3050 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1056 ACCGGC [GA]^CCGG[CT]
DraIII 3901 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1880 GGTCACC G^GT[ACGT]ACC
EcoRI 821 GAATTC G^AATTC
HaeII 2950 AGCGCC [GA]GCGC^[CT]
Hin1I 1148 GACGTC G[GA]^CG[CT]C
HindIII 954 AAGCTT A^AGCTT
NspI 2268 ACATGT [GA]CATG^[CT]
PaeR7I 1996 CTCGAG C^TCGAG
PshAI 1529 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 47 CTGCAG CTGCA^G
RsaI 1403 GTAC GT^AC
SacI 4053 GAGCTC GAGCT^C
StuI 3206 AGGCCT AGG^CCT
XhoI 1996 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 376 CGCG CG^CG
2527 CGCG
AspHI 2709 GAGCAC G[AT]GC[AT]^C
4053 GAGCTC
AspI 1619 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2176 GACTCAGTC
AvaI 1996 CTCGAG C^[CT]CG[GA]G
2410 CCCGAG
BbsI 1526 GAAGAC GAAGAC(2/6)
2804 GTCTTC
BclI 164 TGATCA T^GATCA
1016 TGATCA
BpuAI 1526 GAAGAC GAAGAC(2/6)
2804 GTCTTC
BsaI 246 GAGACC GGTCTC(1/5)
234 GGTCTC
BsiHKAI 2709 GAGCAC G[AT]GC[AT]^C
4053 GAGCTC
BsmBI 877 CGTCTC CGTCTC(1/5)
1150 CGTCTC
BsoBI 1996 CTCGAG C^[CT]CG[GA]G
2410 CCCGAG
BsrDI 1360 GCAATG GCAATG(2/0)
2580 GCAATG
BstUI 376 CGCG CG^CG
2527 CGCG
FbaI 164 TGATCA T^GATCA
1016 TGATCA
MvnI 376 CGCG CG^CG
2527 CGCG
PflFI 1619 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2176 GACTCAGTC
PpuMI 1623 AGGTCCT [GA]G^G[AT]CC[CT]
2189 GGGTCCT
PvuII 3730 CAGCTG CAG^CTG
3819 CAGCTG
SapI 2706 GAAGAGC GCTCTTC(1/4)
3986 GCTCTTC
Tth111I 1619 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2176 GACTCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
AocI ApaI ApoI AvaII BalI
BamHI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII