##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BcgI BciVI BclI BfrI
BlnI BsaBI BsiWI BsmI BspDI
BspHI BspLU11I BsrDI Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoRV EcoT22I
FbaI FseI KpnI KspI MamI
MfeI MluI MunI NheI NotI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 4343 GACGTC GACGT^C
AcsI 1091 GAATTC [GA]^AATT[CT]
AflIII 154 ACACGT A^C[GA][CT]GT
AhdI 4357 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4267 AGCGCT AGC^GCT
ApoI 1091 GAATTC [GA]^AATT[CT]
AspEI 4357 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4305 TGCGCA TGC^GCA
BsiEI 4375 CGACCG CG[GA][CT]^CG
BsmBI 1507 CGTCTC CGTCTC(1/5)
Bsp1407I 4251 TGTACA T^GTACA
BsrBI 248 GAGCGG CCGCTC(-3/-3)
BsrGI 4251 TGTACA T^GTACA
BssHII 448 GCGCGC G^CGCGC
BstEII 3019 GGTGACC G^GT[ACGT]ACC
BstPI 3019 GGTGACC G^GT[ACGT]ACC
DrdI 684 GACCCCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4357 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4267 AGCGCT AGC^GCT
EcoO65I 3019 GGTGACC G^GT[ACGT]ACC
EcoRI 1091 GAATTC G^AATTC
FspI 4305 TGCGCA TGC^GCA
HindIII 4280 AAGCTT A^AGCTT
HpaI 3847 GTTAAC GTT^AAC
NdeI 3207 CATATG CA^TATG
NruI 2531 TCGCGA TCG^CGA
SexAI 2802 ACCAGGT A^CC[AT]GGT
SfiI 3726 GGCCAGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4251 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1144 GTAGAC GT^[AC][GT]AC
2300 GTAGAC
AccIII 1535 TCCGGA T^CCGGA
1740 TCCGGA
Alw44I 2281 GTGCAC G^TGCAC
3102 GTGCAC
AocI 1516 CCTCAGG CC^T[ACGT]AGG
3761 CCTGAGG
ApaLI 2281 GTGCAC G^TGCAC
3102 GTGCAC
BamHI 1472 GGATCC G^GATCC
4100 GGATCC
BbsI 2665 GAAGAC GAAGAC(2/6)
4115 GTCTTC
BbvCI 2961 CCTCAGC CCTCAGC(-5/-2)
3976 GCTGAGG
BglII 965 AGATCT A^GATCT
1977 AGATCT
BlpI 1525 GCTGAGC GC^T[ACGT]AGC
1827 GCTGAGC
Bpu1102I 1525 GCTGAGC GC^T[ACGT]AGC
1827 GCTGAGC
BpuAI 2665 GAAGAC GAAGAC(2/6)
4115 GTCTTC
BseAI 1535 TCCGGA T^CCGGA
1740 TCCGGA
BspEI 1535 TCCGGA T^CCGGA
1740 TCCGGA
BssSI 266 CACGAG CACGAG(-5/-1)
1833 CACGAG
Bsu36I 1516 CCTCAGG CC^T[ACGT]AGG
3761 CCTGAGG
CelII 1525 GCTGAGC GC^T[ACGT]AGC
1827 GCTGAGC
EarI 1380 GAAGAG CTCTTC(1/4)
1557 GAAGAG
Eco81I 1516 CCTCAGG CC^T[ACGT]AGG
3761 CCTGAGG
HincII 1439 GTTGAC GT[CT]^[GA]AC
3847 GTTAAC
HindII 1439 GTTGAC GT[CT]^[GA]AC
3847 GTTAAC
Ksp632I 1380 GAAGAG CTCTTC(1/4)
1557 GAAGAG
MroI 1535 TCCGGA T^CCGGA
1740 TCCGGA
MseI 3569 TTAA T^TAA
3848 TTAA
NaeI 1685 GCCGGC GCC^GGC
1843 GCCGGC
NcoI 49 CCATGG C^CATGG
462 CCATGG
NgoMIV 1685 GCCGGC G^CCGGC
1843 GCCGGC
SmaI 3213 CCCGGG CCC^GGG
3679 CCCGGG
Tru9I 3569 TTAA T^TAA
3848 TTAA
Tsp509I 1092 AATT ^AATT
4065 AATT
XmaCI 3213 CCCGGG C^CCGGG
3679 CCCGGG
XmaI 3213 CCCGGG C^CCGGG
3679 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII