##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI BbsI BcgI
BfrI BglII BpuAI BsiWI BspDI
BspHI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I HgaI HindIII
HpaI KpnI MfeI MluI MunI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfuI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1367 GACGTC GACGT^C
AccIII 2170 TCCGGA T^CCGGA
AcsI 3538 GAATTT [GA]^AATT[CT]
AocI 743 CCTTAGG CC^T[ACGT]AGG
ApoI 3538 GAATTT [GA]^AATT[CT]
Asp700I 1954 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1329 TGCGCA TGC^GCA
AvrII 200 CCTAGG C^CTAGG
BamHI 628 GGATCC G^GATCC
BclI 2046 TGATCA T^GATCA
BlnI 200 CCTAGG C^CTAGG
BsaBI 4010 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2170 TCCGGA [AT]^CCGG[AT]
BseAI 2170 TCCGGA T^CCGGA
BsmI 2996 GCATTC GAATGC(1/-1)
Bsp1407I 3182 TGTACA T^GTACA
BspEI 2170 TCCGGA T^CCGGA
BspLU11I 1045 ACATGT A^CATGT
BsrGI 3182 TGTACA T^GTACA
Bsu36I 743 CCTTAGG CC^T[ACGT]AGG
DraIII 2405 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 743 CCTTAGG CC^T[ACGT]AGG
FbaI 2046 TGATCA T^GATCA
FseI 63 GGCCGGCC GGCCGG^CC
FspI 1329 TGCGCA TGC^GCA
HincII 2574 GTCAAC GT[CT]^[GA]AC
HindII 2574 GTCAAC GT[CT]^[GA]AC
MamI 4010 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2170 TCCGGA T^CCGGA
NdeI 1637 CATATG CA^TATG
PshAI 3106 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2233 ACCTGGT A^CC[AT]GGT
SfiI 13 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2856 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2659 CCCGGG CCC^GGG
SphI 3479 GCATGC GCATG^C
SspBI 3182 TGTACA T^GTACA
XcmI 2104 CCAATGTGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2659 CCCGGG C^CCGGG
XmaI 2659 CCCGGG C^CCGGG
XmnI 1954 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 268 GTAC GT^AC
3183 GTAC
AflIII 1002 ACACGT A^C[GA][CT]GT
1045 ACATGT
Alw44I 2090 GTGCAC G^TGCAC
2520 GTGCAC
ApaLI 2090 GTGCAC G^TGCAC
2520 GTGCAC
BalI 1509 TGGCCA TGG^CCA
3353 TGGCCA
BbrPI 1003 CACGTG CAC^GTG
1709 CACGTG
BlpI 1431 GCTCAGC GC^T[ACGT]AGC
3271 GCTGAGC
Bpu1102I 1431 GCTCAGC GC^T[ACGT]AGC
3271 GCTGAGC
BsaAI 1003 CACGTG [CT]AC^GT[GA]
1709 CACGTG
BsmBI 183 GAGACG CGTCTC(1/5)
2353 GAGACG
BsrDI 1345 CATTGC GCAATG(2/0)
3792 GCAATG
BssSI 553 CACGAG CACGAG(-5/-1)
3967 CTCGTG
BstAPI 935 GCATTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1106 GCAGCTACTGC
CelII 1431 GCTCAGC GC^T[ACGT]AGC
3271 GCTGAGC
MluNI 1509 TGGCCA TGG^CCA
3353 TGGCCA
MscI 1509 TGGCCA TGG^CCA
3353 TGGCCA
MseI 3213 TTAA T^TAA
3307 TTAA
MslI 1594 CACGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3788 CACAGCAATG
PmaCI 1003 CACGTG CAC^GTG
1709 CACGTG
PmlI 1003 CACGTG CAC^GTG
1709 CACGTG
RsaI 268 GTAC GT^AC
3183 GTAC
Tru9I 3213 TTAA T^TAA
3307 TTAA
Tsp509I 3215 AATT ^AATT
3539 AATT
XbaI 863 TCTAGA T^CTAGA
3525 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvCI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XhoII