##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
AscI AseI AsnI AviII BamHI
BbrPI BcgI BfrI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KspI
MamI MluI MroI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfuI SnaBI SphI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 3905 GGTACC G^GTACC
AccII 15 CGCG CG^CG
AclI 285 AACGTT AA^CGTT
AhdI 4343 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1138 GTGCAC G^TGCAC
AocI 3369 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2502 AGCGCT AGC^GCT
ApaLI 1138 GTGCAC G^TGCAC
Asp700I 4255 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3905 GGTACC G^GTACC
AspEI 4343 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1178 TGGCCA TGG^CCA
BbeI 3406 GGCGCC GGCGC^C
BbsI 209 GTCTTC GAAGAC(2/6)
BciVI 1254 GTATCC GTATCC(6/5)
BclI 1130 TGATCA T^GATCA
BglII 2329 AGATCT A^GATCT
BpuAI 209 GTCTTC GAAGAC(2/6)
BsmBI 2708 GAGACG CGTCTC(1/5)
BsrFI 3403 GCCGGC [GA]^CCGG[CT]
BstUI 15 CGCG CG^CG
Bsu36I 3369 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3403 GCCGGC [GA]^CCGG[CT]
DraI 2720 TTTAAA TTT^AAA
DrdI 4117 GACTGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4343 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2502 AGCGCT AGC^GCT
Eco81I 3369 CCTGAGG CC^T[ACGT]AGG
EcoNI 693 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1130 TGATCA T^GATCA
KasI 3406 GGCGCC G^GCGCC
KpnI 3905 GGTACC GGTAC^C
MfeI 2433 CAATTG C^AATTG
MluNI 1178 TGGCCA TGG^CCA
MscI 1178 TGGCCA TGG^CCA
MunI 2433 CAATTG C^AATTG
MvnI 15 CGCG CG^CG
NaeI 3403 GCCGGC GCC^GGC
NarI 3406 GGCGCC GG^CGCC
NgoMIV 3403 GCCGGC G^CCGGC
PaeR7I 2798 CTCGAG C^TCGAG
PshAI 3618 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 285 AACGTT AA^CGTT
SapI 1730 GCTCTTC GCTCTTC(1/4)
SfiI 3113 GGCCCCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3406 GGCGCC GGC^GCC
SgrAI 3402 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 4317 CCCGGG CCC^GGG
SpeI 4268 ACTAGT A^CTAGT
SspI 4486 AATATT AAT^ATT
XhoI 2798 CTCGAG C^TCGAG
XmaCI 4317 CCCGGG C^CCGGG
XmaI 4317 CCCGGG C^CCGGG
XmnI 4255 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 2047 GACGCC G[GA]^CG[CT]C
3406 GGCGCC
AflIII 2732 ACATGT A^C[GA][CT]GT
3499 ACATGT
AvrII 2642 CCTAGG C^CTAGG
3582 CCTAGG
BlnI 2642 CCTAGG C^CTAGG
3582 CCTAGG
BsaHI 2047 GACGCC G[GA]^CG[CT]C
3406 GGCGCC
BsaI 32 GGTCTC GGTCTC(1/5)
2228 GGTCTC
BsmI 4023 GAATGC GAATGC(1/-1)
4306 GAATGC
BspHI 1118 TCATGA T^CATGA
1127 TCATGA
BspLU11I 2732 ACATGT A^CATGT
3499 ACATGT
BsrBI 6 GAGCGG CCGCTC(-3/-3)
1990 GAGCGG
BsrDI 47 GCAATG GCAATG(2/0)
1285 GCAATG
BstXI 1169 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4340 CCAGACTCCTGG
DraIII 1012 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3510 CACCAAGTG
EaeI 75 CGGCCA [CT]^GGCC[GA]
1178 TGGCCA
Hin1I 2047 GACGCC G[GA]^CG[CT]C
3406 GGCGCC
HincII 2881 GTCAAC GT[CT]^[GA]AC
3136 GTCAAC
HindII 2881 GTCAAC GT[CT]^[GA]AC
3136 GTCAAC
MaeII 286 ACGT A^CGT
514 ACGT
RcaI 1118 TCATGA T^CATGA
1127 TCATGA
StuI 195 AGGCCT AGG^CCT
3256 AGGCCT
TaqI 2314 TCGA T^CGA
2799 TCGA
TthHB8I 2314 TCGA T^CGA
2799 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AspI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I Van91I XcmI XhoII