##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BsmI BspDI BspHI
BspLU11I BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DrdI Eco47III
EcoNI EcoRI EcoRV FbaI FseI
FspI HincII HindII HpaI KpnI
MamI MfeI MluI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI ScaI SfuI SpeI
SphI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 4364 AAATTT [GA]^AATT[CT]
AhdI 3788 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 809 GTGCAC G^TGCAC
AocI 1691 CCTGAGG CC^T[ACGT]AGG
ApaLI 809 GTGCAC G^TGCAC
ApoI 4364 AAATTT [GA]^AATT[CT]
AspEI 3788 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 926 CACGTG CAC^GTG
BcgI 3622 CGACCTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1915 GTATCC GTATCC(6/5)
BsiEI 3017 CGGCCG CG[GA][CT]^CG
Bsp1407I 4345 TGTACA T^GTACA
BsrGI 4345 TGTACA T^GTACA
BstEII 1854 GGTCACC G^GT[ACGT]ACC
BstPI 1854 GGTCACC G^GT[ACGT]ACC
Bsu36I 1691 CCTGAGG CC^T[ACGT]AGG
DraI 4361 TTTAAA TTT^AAA
DraIII 2632 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3017 CGGCCG C^GGCCG
Eam1105I 3788 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3017 CGGCCG C^GGCCG
Eco52I 3017 CGGCCG C^GGCCG
Eco81I 1691 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1854 GGTCACC G^GT[ACGT]ACC
EcoT22I 1390 ATGCAT ATGCA^T
KspI 4062 CCGCGG CCGC^GG
NsiI 1390 ATGCAT ATGCA^T
PmaCI 926 CACGTG CAC^GTG
PmlI 926 CACGTG CAC^GTG
SacII 4062 CCGCGG CCGC^GG
SnaBI 530 TACGTA TAC^GTA
SspBI 4345 TGTACA T^GTACA
SspI 4357 AATATT AAT^ATT
SwaI 4360 ATTTAAAT ATTT^AAAT
Tsp509I 4365 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 621 TCCGGA T^CCGGA
1249 TCCGGA
AflIII 925 ACACGT A^C[GA][CT]GT
927 ACGTGT
AspI 1879 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3305 GACACTGTC
BamHI 1107 GGATCC G^GATCC
1409 GGATCC
BbsI 187 GAAGAC GAAGAC(2/6)
3770 GTCTTC
BbvCI 2061 GCTGAGG CCTCAGC(-5/-2)
2867 GCTGAGG
BpuAI 187 GAAGAC GAAGAC(2/6)
3770 GTCTTC
BsaI 302 GAGACC GGTCTC(1/5)
791 GAGACC
BsaWI 621 TCCGGA [AT]^CCGG[AT]
1249 TCCGGA
BseAI 621 TCCGGA T^CCGGA
1249 TCCGGA
BsmBI 2280 CGTCTC CGTCTC(1/5)
3730 GAGACG
BspEI 621 TCCGGA T^CCGGA
1249 TCCGGA
BsrDI 3892 CATTGC GCAATG(2/0)
48 GCAATG
HindIII 70 AAGCTT A^AGCTT
2464 AAGCTT
MroI 621 TCCGGA T^CCGGA
1249 TCCGGA
MseI 4362 TTAA T^TAA
4393 TTAA
PflFI 1879 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3305 GACACTGTC
PflMI 2882 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3679 CCACAGCCTGG
SapI 1321 GAAGAGC GCTCTTC(1/4)
2922 GCTCTTC
SbfI 1701 CCTGCAGG CCTGCA^GG
1720 CCTGCAGG
SexAI 219 ACCTGGT A^CC[AT]GGT
4077 ACCTGGT
SfiI 1013 GGCCTAAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3904 GGCCCGCCAGGCC
SgrAI 1417 CGCCGGCG C[GA]^CCGG[CT]G
3037 CGCCGGCG
Sse8387I 1701 CCTGCAGG CCTGCA^GG
1720 CCTGCAGG
TaqI 690 TCGA T^CGA
4301 TCGA
TfiI 319 GATTC G^A[AT]TC
2332 GATTC
Tru9I 4362 TTAA T^TAA
4393 TTAA
Tth111I 1879 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3305 GACACTGTC
TthHB8I 690 TCGA T^CGA
4301 TCGA
Van91I 2882 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3679 CCACAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TseI Tsp45I TspRI
XcmI XhoII XmaCI XmaI