##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AspI AviII BamHI BbrPI
BbvCI BcgI BfrI BglI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FseI FspI HgaI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 3906 GGTACC G^GTACC
AcyI 1240 GGCGCC G[GA]^CG[CT]C
AhdI 200 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1187 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 804 CCTAAGG CC^T[ACGT]AGG
Asp718I 3906 GGTACC G^GTACC
AspEI 200 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 297 CCTAGG C^CTAGG
BbeI 1240 GGCGCC GGCGC^C
BciVI 2756 GGATAC GTATCC(6/5)
BclI 3214 TGATCA T^GATCA
BglII 511 AGATCT A^GATCT
BlnI 297 CCTAGG C^CTAGG
BsaHI 1240 GGCGCC G[GA]^CG[CT]C
BspLU11I 1204 ACATGT A^CATGT
BssSI 31 CTCGTG CACGAG(-5/-1)
BstAPI 563 GCAAGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 804 CCTAAGG CC^T[ACGT]AGG
CfoI 1241 GCGC GCG^C
Eam1105I 200 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 804 CCTAAGG CC^T[ACGT]AGG
EcoNI 233 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 368 CCCGC CCCGC(4/6)
FbaI 3214 TGATCA T^GATCA
HaeII 1240 GGCGCC [GA]GCGC^[CT]
HhaI 1241 GCGC GCG^C
Hin1I 1240 GGCGCC G[GA]^CG[CT]C
HinP1I 1241 GCGC G^CGC
HindIII 2922 AAGCTT A^AGCTT
HpaI 4212 GTTAAC GTT^AAC
KasI 1240 GGCGCC G^GCGCC
KpnI 3906 GGTACC GGTAC^C
NarI 1240 GGCGCC GG^CGCC
NheI 371 GCTAGC G^CTAGC
PflMI 1988 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 621 GAGCTC GAGCT^C
SalI 961 GTCGAC G^TCGAC
ScaI 638 AGTACT AGT^ACT
SexAI 3909 ACCTGGT A^CC[AT]GGT
SfoI 1240 GGCGCC GGC^GCC
StuI 1886 AGGCCT AGG^CCT
SwaI 4432 ATTTAAAT ATTT^AAAT
Van91I 1988 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3396 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 204 ACGTGT A^C[GA][CT]GT
1204 ACATGT
AseI 2338 ATTAAT AT^TAAT
4477 ATTAAT
AsnI 2338 ATTAAT AT^TAAT
4477 ATTAAT
BalI 1395 TGGCCA TGG^CCA
1792 TGGCCA
BlpI 251 GCTGAGC GC^T[ACGT]AGC
1556 GCTCAGC
Bpu1102I 251 GCTGAGC GC^T[ACGT]AGC
1556 GCTCAGC
Bsp1407I 2541 TGTACA T^GTACA
2549 TGTACA
BspMI 2090 GCAGGT ACCTGC(4/8)
4314 GCAGGT
BsrDI 4067 CATTGC GCAATG(2/0)
3547 GCAATG
BsrGI 2541 TGTACA T^GTACA
2549 TGTACA
BstXI 1783 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2820 CCAGTGTGATGG
BstYI 106 AGATCC [GA]^GATC[CT]
511 AGATCT
CelII 251 GCTGAGC GC^T[ACGT]AGC
1556 GCTCAGC
EaeI 1395 TGGCCA [CT]^GGCC[GA]
1792 TGGCCA
EcoT22I 3357 ATGCAT ATGCA^T
4469 ATGCAT
MflI 106 AGATCC [GA]^GATC[CT]
511 AGATCT
MluNI 1395 TGGCCA TGG^CCA
1792 TGGCCA
MscI 1395 TGGCCA TGG^CCA
1792 TGGCCA
NsiI 3357 ATGCAT ATGCA^T
4469 ATGCAT
PpuMI 952 GGGACCT [GA]G^G[AT]CC[CT]
1267 AGGACCT
PshAI 734 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACCTTTGTC
PshBI 2338 ATTAAT AT^TAAT
4477 ATTAAT
SapI 2085 GAAGAGC GCTCTTC(1/4)
476 GCTCTTC
SmaI 1342 CCCGGG CCC^GGG
2152 CCCGGG
SmlI 1509 CTTGAG C^T[CT][GA]AG
2732 CTTGAG
SspBI 2541 TGTACA T^GTACA
2549 TGTACA
XcmI 2198 CCAGGGTGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4370 CCATTTTTATTGTGG
XhoII 106 AGATCC [GA]^GATC[CT]
511 AGATCT
XmaCI 1342 CCCGGG C^CCGGG
2152 CCCGGG
XmaI 1342 CCCGGG C^CCGGG
2152 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI