##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BclI
BfrI BlnI BsiEI BsiWI BspDI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 2427 AACGTT AA^CGTT
AcsI 3770 AAATTC [GA]^AATT[CT]
Aor51HI 4363 AGCGCT AGC^GCT
ApoI 3770 AAATTC [GA]^AATT[CT]
BamHI 2420 GGATCC G^GATCC
BbeI 1843 GGCGCC GGCGC^C
BbrPI 334 CACGTG CAC^GTG
BbvCI 4346 GCTGAGG CCTCAGC(-5/-2)
BciVI 248 GGATAC GTATCC(6/5)
BglII 777 AGATCT A^GATCT
BsaAI 334 CACGTG [CT]AC^GT[GA]
BsaBI 249 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1860 CGTCTC CGTCTC(1/5)
Bsp1407I 338 TGTACA T^GTACA
BspMI 3742 ACCTGC ACCTGC(4/8)
BsrDI 1020 CATTGC GCAATG(2/0)
BsrGI 338 TGTACA T^GTACA
BstEII 3130 GGTTACC G^GT[ACGT]ACC
BstPI 3130 GGTTACC G^GT[ACGT]ACC
BstXI 476 CCAACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1850 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4363 AGCGCT AGC^GCT
EcoO65I 3130 GGTTACC G^GT[ACGT]ACC
HindIII 1549 AAGCTT A^AGCTT
HpaI 202 GTTAAC GTT^AAC
KasI 1843 GGCGCC G^GCGCC
MamI 249 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1843 GGCGCC GG^CGCC
PflMI 320 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 334 CACGTG CAC^GTG
PmlI 334 CACGTG CAC^GTG
Psp1406I 2427 AACGTT AA^CGTT
SbfI 2116 CCTGCAGG CCTGCA^GG
SexAI 3214 ACCTGGT A^CC[AT]GGT
SfoI 1843 GGCGCC GGC^GCC
SmaI 1975 CCCGGG CCC^GGG
Sse8387I 2116 CCTGCAGG CCTGCA^GG
SspBI 338 TGTACA T^GTACA
SspI 182 AATATT AAT^ATT
Van91I 320 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1975 CCCGGG C^CCGGG
XmaI 1975 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 949 GTCTAC GT^[AC][GT]AC
3991 GTCTAC
AccIII 703 TCCGGA T^CCGGA
884 TCCGGA
ApaI 2228 GGGCCC GGGCC^C
2695 GGGCCC
AspI 3001 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3985 GACACAGTC
BcgI 1242 GCAGCCGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1875 GCAGACGGATCG
BfaI 3241 CTAG C^TAG
3722 CTAG
BsaWI 703 TCCGGA [AT]^CCGG[AT]
884 TCCGGA
BseAI 703 TCCGGA T^CCGGA
884 TCCGGA
BspEI 703 TCCGGA T^CCGGA
884 TCCGGA
BspLU11I 209 ACATGT A^CATGT
1167 ACATGT
BstAPI 717 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1305 GCAGCAAGTGC
HaeII 1843 GGCGCC [GA]GCGC^[CT]
4363 AGCGCT
HgaI 1999 GACGC GACGC(5/10)
2260 GCGTC
HincII 202 GTTAAC GT[CT]^[GA]AC
4129 GTCAAC
HindII 202 GTTAAC GT[CT]^[GA]AC
4129 GTCAAC
KspI 1527 CCGCGG CCGC^GG
2925 CCGCGG
MaeI 3241 CTAG C^TAG
3722 CTAG
MroI 703 TCCGGA T^CCGGA
884 TCCGGA
NaeI 1840 GCCGGC GCC^GGC
2250 GCCGGC
NcoI 2360 CCATGG C^CATGG
4195 CCATGG
NgoMIV 1840 GCCGGC G^CCGGC
2250 GCCGGC
PflFI 3001 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3985 GACACAGTC
SacII 1527 CCGCGG CCGC^GG
2925 CCGCGG
Tth111I 3001 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3985 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII