##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI AscI AspEI AvrII
BbeI BbrPI BclI BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoRV FbaI
FseI HgaI Hin1I HpaI KasI
KspI MfeI MluI MroI MunI
NarI NdeI NotI NruI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3081 GGTACC G^GTACC
AccI 715 GTAGAC GT^[AC][GT]AC
AccII 1071 CGCG CG^CG
AflII 3704 CTTAAG C^TTAAG
Asp718I 3081 GGTACC G^GTACC
AviII 1905 TGCGCA TGC^GCA
BalI 2173 TGGCCA TGG^CCA
BbsI 1711 GAAGAC GAAGAC(2/6)
BbvCI 1303 CCTCAGC CCTCAGC(-5/-2)
BfrI 3704 CTTAAG C^TTAAG
BglI 613 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3314 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3314 GCTCAGC GC^T[ACGT]AGC
BpuAI 1711 GAAGAC GAAGAC(2/6)
BsaBI 335 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1268 GAGACC GGTCTC(1/5)
BsaWI 845 ACCGGA [AT]^CCGG[AT]
BspHI 2835 TCATGA T^CATGA
BsrBI 1928 CCGCTC CCGCTC(-3/-3)
BstAPI 1591 GCAACAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 957 TTCGAA TT^CGAA
BstUI 1071 CGCG CG^CG
CelII 3314 GCTCAGC GC^T[ACGT]AGC
EcoRI 3968 GAATTC G^AATTC
FspI 1905 TGCGCA TGC^GCA
KpnI 3081 GGTACC GGTAC^C
MamI 335 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2173 TGGCCA TGG^CCA
MscI 2173 TGGCCA TGG^CCA
MvnI 1071 CGCG CG^CG
NaeI 2133 GCCGGC GCC^GGC
NgoMIV 2133 GCCGGC G^CCGGC
NheI 4090 GCTAGC G^CTAGC
NspV 957 TTCGAA TT^CGAA
PaeR7I 1985 CTCGAG C^TCGAG
PpuMI 903 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 766 CTGCAG CTGCA^G
PvuII 1886 CAGCTG CAG^CTG
RcaI 2835 TCATGA T^CATGA
SacI 2150 GAGCTC GAGCT^C
SfuI 957 TTCGAA TT^CGAA
SpeI 3551 ACTAGT A^CTAGT
SphI 2988 GCATGC GCATG^C
SspI 3176 AATATT AAT^ATT
StuI 2142 AGGCCT AGG^CCT
SwaI 3248 ATTTAAAT ATTT^AAAT
XhoI 1985 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 263 GTGCAC G^TGCAC
2261 GTGCAC
AlwNI 1049 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1835 CAGTACCTG
Aor51HI 558 AGCGCT AGC^GCT
1948 AGCGCT
ApaI 1680 GGGCCC GGGCC^C
2160 GGGCCC
ApaLI 263 GTGCAC G^TGCAC
2261 GTGCAC
AseI 270 ATTAAT AT^TAAT
3425 ATTAAT
AsnI 270 ATTAAT AT^TAAT
3425 ATTAAT
Asp700I 919 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1861 GAAGGAGTTC
AspI 1270 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2498 GACAGTGTC
AvaI 1985 CTCGAG C^[CT]CG[GA]G
2091 CCCGAG
BcgI 3478 CGAGTTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1908 GCACAGTGTTCG
BciVI 2371 GGATAC GTATCC(6/5)
4320 GTATCC
BcnI 1761 CCCGG CC^[GC]GG
2069 CCCGG
BsoBI 1985 CTCGAG C^[CT]CG[GA]G
2091 CCCGAG
Bsp1407I 2639 TGTACA T^GTACA
3182 TGTACA
BsrDI 3484 CATTGC GCAATG(2/0)
461 GCAATG
BsrFI 1881 ACCGGC [GA]^CCGG[CT]
2133 GCCGGC
BsrGI 2639 TGTACA T^GTACA
3182 TGTACA
BstEII 72 GGTAACC G^GT[ACGT]ACC
3791 GGTGACC
BstPI 72 GGTAACC G^GT[ACGT]ACC
3791 GGTGACC
BstXI 91 CCAAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
893 CCATCTCTCTGG
Cfr10I 1881 ACCGGC [GA]^CCGG[CT]
2133 GCCGGC
DrdI 1081 GACAACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1289 GACACAACTGTC
Eco47III 558 AGCGCT AGC^GCT
1948 AGCGCT
EcoO65I 72 GGTAACC G^GT[ACGT]ACC
3791 GGTGACC
EcoT22I 2289 ATGCAT ATGCA^T
3870 ATGCAT
HincII 841 GTTGAC GT[CT]^[GA]AC
2125 GTTGAC
HindII 841 GTTGAC GT[CT]^[GA]AC
2125 GTTGAC
HindIII 648 AAGCTT A^AGCTT
2762 AAGCTT
MspA1I 1886 CAGCTG C[AC]G^C[GT]G
2242 CAGCGG
NciI 1761 CCCGG CC^[GC]GG
2069 CCCGG
NcoI 1935 CCATGG C^CATGG
2645 CCATGG
NsiI 2289 ATGCAT ATGCA^T
3870 ATGCAT
PacI 4109 TTAATTAA TTAAT^TAA
4128 TTAATTAA
PflFI 1270 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2498 GACAGTGTC
PshBI 270 ATTAAT AT^TAAT
3425 ATTAAT
SexAI 2227 ACCTGGT A^CC[AT]GGT
3977 ACCAGGT
SspBI 2639 TGTACA T^GTACA
3182 TGTACA
TaqI 958 TCGA T^CGA
1986 TCGA
Tth111I 1270 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2498 GACAGTGTC
TthHB8I 958 TCGA T^CGA
1986 TCGA
XmnI 919 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1861 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaII BamHI
BanI BanII BbvI BfaI BglII
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII