##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AscI Asp700I Asp718I BbsI BcgI
BfrI BglII BpuAI BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraIII EcoT22I
HincII HindII HpaI KpnI MamI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI Psp1406I PvuI RcaI RsrII
SalI SexAI SfiI SfuI SgrAI
SpeI SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 842 TCCGGA T^CCGGA
AcsI 1998 GAATTC [GA]^AATT[CT]
AgeI 3790 ACCGGT A^CCGGT
AhdI 118 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1998 GAATTC [GA]^AATT[CT]
AseI 1974 ATTAAT AT^TAAT
AsnI 1974 ATTAAT AT^TAAT
AspEI 118 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 473 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 492 TGCGCA TGC^GCA
AvrII 2442 CCTAGG C^CTAGG
BamHI 2839 GGATCC G^GATCC
BciVI 3865 GGATAC GTATCC(6/5)
BlnI 2442 CCTAGG C^CTAGG
BseAI 842 TCCGGA T^CCGGA
BsmBI 2469 CGTCTC CGTCTC(1/5)
BspEI 842 TCCGGA T^CCGGA
BstEII 3556 GGTCACC G^GT[ACGT]ACC
BstPI 3556 GGTCACC G^GT[ACGT]ACC
BstXI 50 CCAAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 3404 TTTAAA TTT^AAA
DrdI 721 GACCAACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 118 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3556 GGTCACC G^GT[ACGT]ACC
EcoRI 1998 GAATTC G^AATTC
EcoRV 3894 GATATC GAT^ATC
FseI 2406 GGCCGGCC GGCCGG^CC
FspI 492 TGCGCA TGC^GCA
HindIII 3176 AAGCTT A^AGCTT
MroI 842 TCCGGA T^CCGGA
NdeI 3496 CATATG CA^TATG
NspI 1029 GCATGC [GA]CATG^[CT]
PflFI 473 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3790 ACCGGT A^CCGGT
PshBI 1974 ATTAAT AT^TAAT
SapI 502 GAAGAGC GCTCTTC(1/4)
SbfI 2455 CCTGCAGG CCTGCA^GG
ScaI 2102 AGTACT AGT^ACT
SmlI 2618 CTCAAG C^T[CT][GA]AG
SnaBI 2854 TACGTA TAC^GTA
SphI 1029 GCATGC GCATG^C
Sse8387I 2455 CCTGCAGG CCTGCA^GG
Tsp509I 1999 AATT ^AATT
Tth111I 473 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1609 ACGTGT A^C[GA][CT]GT
2213 ACACGT
Alw44I 1342 GTGCAC G^TGCAC
1387 GTGCAC
Aor51HI 865 AGCGCT AGC^GCT
1587 AGCGCT
ApaLI 1342 GTGCAC G^TGCAC
1387 GTGCAC
BalI 711 TGGCCA TGG^CCA
3749 TGGCCA
BclI 2950 TGATCA T^GATCA
3982 TGATCA
BsrDI 307 CATTGC GCAATG(2/0)
1884 CATTGC
BssSI 1596 CACGAG CACGAG(-5/-1)
1941 CTCGTG
EagI 1102 CGGCCG C^GGCCG
2405 CGGCCG
EarI 502 GAAGAG CTCTTC(1/4)
569 GAAGAG
EclXI 1102 CGGCCG C^GGCCG
2405 CGGCCG
Eco47III 865 AGCGCT AGC^GCT
1587 AGCGCT
Eco52I 1102 CGGCCG C^GGCCG
2405 CGGCCG
FbaI 2950 TGATCA T^GATCA
3982 TGATCA
Ksp632I 502 GAAGAG CTCTTC(1/4)
569 GAAGAG
MluNI 711 TGGCCA TGG^CCA
3749 TGGCCA
MscI 711 TGGCCA TGG^CCA
3749 TGGCCA
PshAI 1196 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2695 GACTCCAGTC
SacI 10 GAGCTC GAGCT^C
3721 GAGCTC
StuI 1600 AGGCCT AGG^CCT
3008 AGGCCT
TaqI 1023 TCGA T^CGA
2355 TCGA
TthHB8I 1023 TCGA T^CGA
2355 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbrPI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StyI
TfiI Tru9I TseI Tsp45I TspRI
Van91I XcmI XhoII XmaCI XmaI