##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
AseI AsnI Asp700I AvrII BamHI
BclI BfrI BlnI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BsrDI BssHII BstBI ClaI
CpoI DraI DraIII EcoRV EcoT22I
FbaI HpaI KspI MamI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfuI SnaBI SpeI SphI SspI
SwaI TfiI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 3943 GAATTC [GA]^AATT[CT]
AflIII 2329 ACGTGT A^C[GA][CT]GT
Alw44I 3071 GTGCAC G^TGCAC
Aor51HI 4194 AGCGCT AGC^GCT
ApaLI 3071 GTGCAC G^TGCAC
ApoI 3943 GAATTC [GA]^AATT[CT]
BbeI 79 GGCGCC GGCGC^C
BcgI 2145 GCAGCTATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1755 AGATCT A^GATCT
BssSI 216 CACGAG CACGAG(-5/-1)
Bst1107I 799 GTATAC GTA^TAC
BstZ17I 799 GTATAC GTA^TAC
DrdI 2402 GACTTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4194 AGCGCT AGC^GCT
EcoRI 3943 GAATTC G^AATTC
FseI 3846 GGCCGGCC GGCCGG^CC
HindIII 4396 AAGCTT A^AGCTT
KasI 79 GGCGCC G^GCGCC
NarI 79 GGCGCC GG^CGCC
NheI 1999 GCTAGC G^CTAGC
PaeR7I 3223 CTCGAG C^TCGAG
SapI 4411 GAAGAGC GCTCTTC(1/4)
SexAI 1020 ACCAGGT A^CC[AT]GGT
SfoI 79 GGCGCC GGC^GCC
SmlI 3223 CTCGAG C^T[CT][GA]AG
XhoI 3223 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 588 GGTACC G^GTACC
2702 GGTACC
AccI 799 GTATAC GT^[AC][GT]AC
1306 GTAGAC
AhdI 1846 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3298 GACACAAGGTC
AlwI 2501 GATCC GGATC(4/5)
3130 GGATC
Asp718I 588 GGTACC G^GTACC
2702 GGTACC
AspEI 1846 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3298 GACACAAGGTC
AspI 2173 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4009 GACTTAGTC
AviII 433 TGCGCA TGC^GCA
1621 TGCGCA
BsmI 992 GAATGC GAATGC(1/-1)
2999 GAATGC
BstAPI 4064 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4332 GCATCTGCTGC
BstEII 3086 GGTCACC G^GT[ACGT]ACC
3431 GGTCACC
BstPI 3086 GGTCACC G^GT[ACGT]ACC
3431 GGTCACC
EagI 3845 CGGCCG C^GGCCG
3849 CGGCCG
Eam1105I 1846 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3298 GACACAAGGTC
EclXI 3845 CGGCCG C^GGCCG
3849 CGGCCG
Eco52I 3845 CGGCCG C^GGCCG
3849 CGGCCG
EcoO65I 3086 GGTCACC G^GT[ACGT]ACC
3431 GGTCACC
FspI 433 TGCGCA TGC^GCA
1621 TGCGCA
HincII 2089 GTCAAC GT[CT]^[GA]AC
2449 GTCAAC
HindII 2089 GTCAAC GT[CT]^[GA]AC
2449 GTCAAC
KpnI 588 GGTACC GGTAC^C
2702 GGTACC
MslI 214 CACACGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2352 CACCGGCGTG
NspI 648 ACATGC [GA]CATG^[CT]
2893 GCATGT
PflFI 2173 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4009 GACTTAGTC
PshAI 173 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2648 GACCTGGGTC
SbfI 323 CCTGCAGG CCTGCA^GG
3050 CCTGCAGG
SgrAI 127 CGCCGGCG C[GA]^CCGG[CT]G
2352 CACCGGCG
Sse8387I 323 CCTGCAGG CCTGCA^GG
3050 CCTGCAGG
Tsp509I 3944 AATT ^AATT
4391 AATT
Tth111I 2173 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4009 GACTTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SspBI
StuI StyI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI