##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI Asp718I
AspEI AviII AvrII BbrPI BlnI
BlpI Bpu1102I BsaWI BseAI BsiWI
BspEI BsrBI BssSI Bst1107I BstEII
BstPI BstZ17I CelII Eam1105I Eco47III
EcoO65I FseI FspI KpnI KspI
MfeI MluI MroI MunI NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmlI PshAI Psp1406I PvuI
SacI SacII SalI SapI SbfI
SexAI SfiI SgrAI SpeI SphI
Sse8387I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1721 GTAGAC GT^[AC][GT]AC
AlwNI 975 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3599 CCTCAGG CC^T[ACGT]AGG
AscI 167 GGCGCGCC GG^CGCGCC
Asp700I 2391 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3460 CCCGGG C^[CT]CG[GA]G
BcgI 1756 GCAGGCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1962 AGATCT A^GATCT
BsaAI 2417 TACGTA [CT]AC^GT[GA]
BsaBI 686 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 427 GAGGAG GAGGAG(10/8)
BsiEI 53 CGGCCG CG[GA][CT]^CG
BsmBI 2763 CGTCTC CGTCTC(1/5)
BsoBI 3460 CCCGGG C^[CT]CG[GA]G
BspDI 693 ATCGAT AT^CGAT
BstAPI 4245 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1164 TTCGAA TT^CGAA
BstF5I 4204 GGATG GGATG(2/0)
Bsu36I 3599 CCTCAGG CC^T[ACGT]AGG
ClaI 693 ATCGAT AT^CGAT
CpoI 17 CGGTCCG CG^G[AT]CCG
DrdI 2303 GACTGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 53 CGGCCG C^GGCCG
EclXI 53 CGGCCG C^GGCCG
Eco52I 53 CGGCCG C^GGCCG
Eco81I 3599 CCTCAGG CC^T[ACGT]AGG
EcoNI 4064 CCTTATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1061 GAATTC G^AATTC
EcoRV 2067 GATATC GAT^ATC
EcoT22I 1918 ATGCAT ATGCA^T
FokI 4204 GGATG GGATG(9/13)
MamI 686 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 145 GCCGGC GCC^GGC
NcoI 1457 CCATGG C^CATGG
NdeI 3972 CATATG CA^TATG
NgoMIV 145 GCCGGC G^CCGGC
NsiI 1918 ATGCAT ATGCA^T
NspV 1164 TTCGAA TT^CGAA
PpuMI 1525 AGGACCT [GA]G^G[AT]CC[CT]
PstI 2186 CTGCAG CTGCA^G
RsrII 17 CGGTCCG CG^G[AT]CCG
ScaI 1017 AGTACT AGT^ACT
SfuI 1164 TTCGAA TT^CGAA
SmaI 3460 CCCGGG CCC^GGG
SnaBI 2417 TACGTA TAC^GTA
StuI 4623 AGGCCT AGG^CCT
XmaCI 3460 CCCGGG C^CCGGG
XmaI 3460 CCCGGG C^CCGGG
XmnI 2391 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 142 GGCGCC G[GA]^CG[CT]C
952 GGCGCC
ApaI 70 GGGCCC GGGCC^C
86 GGGCCC
AspI 1724 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2924 GACTGTGTC
AvaII 18 GGTCC G^G[AT]CC
1526 GGACC
BamHI 493 GGATCC G^GATCC
1692 GGATCC
BanI 142 GGCGCC G^G[CT][GA]CC
952 GGCGCC
BbeI 142 GGCGCC GGCGC^C
952 GGCGCC
BbsI 418 GTCTTC GAAGAC(2/6)
4275 GTCTTC
BciVI 3897 GGATAC GTATCC(6/5)
3356 GTATCC
BclI 582 TGATCA T^GATCA
2446 TGATCA
BglI 72 GCCCTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
149 GCCCTTGTGGC
BpuAI 418 GTCTTC GAAGAC(2/6)
4275 GTCTTC
BsaHI 142 GGCGCC G[GA]^CG[CT]C
952 GGCGCC
BsgI 242 CTGCAC GTGCAG(16/14)
803 GTGCAG
BsmFI 222 GGGAC GGGAC(10/14)
788 GGGAC
Bsp1407I 1324 TGTACA T^GTACA
4122 TGTACA
BsrFI 145 GCCGGC [GA]^CCGG[CT]
1572 ACCGGC
BsrGI 1324 TGTACA T^GTACA
4122 TGTACA
BssHII 2 GCGCGC G^CGCGC
168 GCGCGC
BstXI 2818 CCAGCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3627 CCAAGGTGCTGG
Cfr10I 145 GCCGGC [GA]^CCGG[CT]
1572 ACCGGC
DraIII 245 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
614 CACACTGTG
FbaI 582 TGATCA T^GATCA
2446 TGATCA
HaeII 142 GGCGCC [GA]GCGC^[CT]
952 GGCGCC
HgaI 1368 GACGC GACGC(5/10)
1950 GACGC
Hin1I 142 GGCGCC G[GA]^CG[CT]C
952 GGCGCC
HincII 808 GTTAAC GT[CT]^[GA]AC
995 GTTAAC
HindII 808 GTTAAC GT[CT]^[GA]AC
995 GTTAAC
HindIII 1426 AAGCTT A^AGCTT
2392 AAGCTT
HpaI 808 GTTAAC GTT^AAC
995 GTTAAC
KasI 142 GGCGCC G^GCGCC
952 GGCGCC
MaeII 1336 ACGT A^CGT
2418 ACGT
NarI 142 GGCGCC GG^CGCC
952 GGCGCC
PflFI 1724 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2924 GACTGTGTC
PflMI 283 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1636 CCAAAAATTGG
PmeI 2982 GTTTAAAC GTTT^AAAC
3276 GTTTAAAC
PvuII 2377 CAGCTG CAG^CTG
3996 CAGCTG
SfoI 142 GGCGCC GGC^GCC
952 GGCGCC
SspBI 1324 TGTACA T^GTACA
4122 TGTACA
SspI 341 AATATT AAT^ATT
2969 AATATT
SwaI 1281 ATTTAAAT ATTT^AAAT
2964 ATTTAAAT
Tth111I 1724 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2924 GACTGTGTC
Van91I 283 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1636 CCAAAAATTGG
XbaI 1541 TCTAGA T^CTAGA
1657 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AseI
AsnI AspHI BalI BanII BbvCI
BbvI BcnI BfaI BfrI BmyI
BpmI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII