##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccIII AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbrPI BcgI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI BstBI CelII Cfr10I
ClaI CpoI DsaI EagI Eam1105I
EclXI Eco52I EcoRI FseI FspI
HpaI KspI MamI MfeI MluI
MroI MunI NaeI NcoI NgoMIV
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacI SacII
SalI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SwaI Tth111I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 3163 GGTACC G^GTACC
AccII 3231 CGCG CG^CG
AclI 1362 AACGTT AA^CGTT
Aor51HI 650 AGCGCT AGC^GCT
Asp718I 3163 GGTACC G^GTACC
BalI 107 TGGCCA TGG^CCA
BamHI 1059 GGATCC G^GATCC
BbeI 3507 GGCGCC GGCGC^C
BbvCI 3999 GCTGAGG CCTCAGC(-5/-2)
BclI 2218 TGATCA T^GATCA
BglII 3728 AGATCT A^GATCT
BsaWI 3100 TCCGGT [AT]^CCGG[AT]
Bsp1407I 851 TGTACA T^GTACA
BspHI 2966 TCATGA T^CATGA
BsrDI 3480 GCAATG GCAATG(2/0)
BsrGI 851 TGTACA T^GTACA
Bst1107I 2892 GTATAC GTA^TAC
BstUI 3231 CGCG CG^CG
BstZ17I 2892 GTATAC GTA^TAC
DraIII 3222 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3666 GACTTAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 650 AGCGCT AGC^GCT
EcoRV 657 GATATC GAT^ATC
EcoT14I 3076 CCAAGG C^C[AT][AT]GG
FbaI 2218 TGATCA T^GATCA
HgaI 4424 GACGC GACGC(5/10)
HincII 585 GTCAAC GT[CT]^[GA]AC
HindII 585 GTCAAC GT[CT]^[GA]AC
KasI 3507 GGCGCC G^GCGCC
KpnI 3163 GGTACC GGTAC^C
MluNI 107 TGGCCA TGG^CCA
MscI 107 TGGCCA TGG^CCA
MvnI 3231 CGCG CG^CG
NarI 3507 GGCGCC GG^CGCC
NheI 3453 GCTAGC G^CTAGC
PaeR7I 4400 CTCGAG C^TCGAG
PflMI 3191 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1362 AACGTT AA^CGTT
RcaI 2966 TCATGA T^CATGA
SbfI 1839 CCTGCAGG CCTGCA^GG
ScaI 3834 AGTACT AGT^ACT
SexAI 580 ACCAGGT A^CC[AT]GGT
SfoI 3507 GGCGCC GGC^GCC
Sse8387I 1839 CCTGCAGG CCTGCA^GG
SspBI 851 TGTACA T^GTACA
StuI 1932 AGGCCT AGG^CCT
StyI 3076 CCAAGG C^C[AT][AT]GG
Van91I 3191 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3713 CCAGCACGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4400 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 3507 GGCGCC G[GA]^CG[CT]C
4424 GACGCC
AocI 2311 CCTGAGG CC^T[ACGT]AGG
4428 CCTCAGG
BanII 1407 GAGCCC G[GA]GC[CT]^C
3570 GAGCCC
BciVI 2777 GGATAC GTATCC(6/5)
2032 GTATCC
BsaHI 3507 GGCGCC G[GA]^CG[CT]C
4424 GACGCC
BstEII 529 GGTAACC G^GT[ACGT]ACC
3888 GGTCACC
BstPI 529 GGTAACC G^GT[ACGT]ACC
3888 GGTCACC
BstXI 2037 CCAGATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4104 CCACAAAGATGG
Bsu36I 2311 CCTGAGG CC^T[ACGT]AGG
4428 CCTCAGG
Eco81I 2311 CCTGAGG CC^T[ACGT]AGG
4428 CCTCAGG
EcoNI 865 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2511 CCTTTGTCAGG
EcoO65I 529 GGTAACC G^GT[ACGT]ACC
3888 GGTCACC
Hin1I 3507 GGCGCC G[GA]^CG[CT]C
4424 GACGCC
MaeII 1363 ACGT A^CGT
4069 ACGT
NdeI 2408 CATATG CA^TATG
2662 CATATG
SapI 262 GAAGAGC GCTCTTC(1/4)
2338 GCTCTTC
TaqI 3558 TCGA T^CGA
4401 TCGA
TthHB8I 3558 TCGA T^CGA
4401 TCGA
XbaI 2935 TCTAGA T^CTAGA
3961 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII