##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BbsI BciVI BclI
BglII BpuAI BsaBI BseAI BsiWI
BsmI BspDI BspEI BspHI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 4114 AAATTT [GA]^AATT[CT]
AflII 3822 CTTAAG C^TTAAG
Aor51HI 620 AGCGCT AGC^GCT
ApoI 4114 AAATTT [GA]^AATT[CT]
Asp700I 3778 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2960 CCTAGG C^CTAGG
BbeI 1494 GGCGCC GGCGC^C
BcgI 2496 CGATGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3822 CTTAAG C^TTAAG
BlnI 2960 CCTAGG C^CTAGG
BlpI 471 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 471 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 832 TGTACA T^GTACA
BspLU11I 3520 ACATGT A^CATGT
BsrDI 3575 CATTGC GCAATG(2/0)
BsrGI 832 TGTACA T^GTACA
BssHII 259 GCGCGC G^CGCGC
BstEII 1552 GGTCACC G^GT[ACGT]ACC
BstPI 1552 GGTCACC G^GT[ACGT]ACC
CelII 471 GCTGAGC GC^T[ACGT]AGC
Eco47III 620 AGCGCT AGC^GCT
EcoO65I 1552 GGTCACC G^GT[ACGT]ACC
KasI 1494 GGCGCC G^GCGCC
MluI 1183 ACGCGT A^CGCGT
NarI 1494 GGCGCC GG^CGCC
PaeR7I 2487 CTCGAG C^TCGAG
PshAI 2305 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2811 GAGCTC GAGCT^C
SapI 2613 GCTCTTC GCTCTTC(1/4)
SfoI 1494 GGCGCC GGC^GCC
SmaI 1761 CCCGGG CCC^GGG
SspBI 832 TGTACA T^GTACA
XhoI 2487 CTCGAG C^TCGAG
XmaCI 1761 CCCGGG C^CCGGG
XmaI 1761 CCCGGG C^CCGGG
XmnI 3778 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 1547 CCTGAGG CC^T[ACGT]AGG
1989 CCTGAGG
BglI 1988 GCCTGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2484 GCCCTCGAGGC
BsaI 861 GAGACC GGTCTC(1/5)
2326 GGTCTC
BsaWI 1147 ACCGGA [AT]^CCGG[AT]
2647 ACCGGA
BsmBI 117 GAGACG CGTCTC(1/5)
1235 GAGACG
BssSI 1028 CACGAG CACGAG(-5/-1)
3113 CTCGTG
BstYI 197 GGATCT [GA]^GATC[CT]
3739 GGATCT
Bsu36I 1547 CCTGAGG CC^T[ACGT]AGG
1989 CCTGAGG
DraI 4106 TTTAAA TTT^AAA
4118 TTTAAA
EagI 136 CGGCCG C^GGCCG
874 CGGCCG
EarI 672 CTCTTC CTCTTC(1/4)
2614 CTCTTC
EclXI 136 CGGCCG C^GGCCG
874 CGGCCG
Eco52I 136 CGGCCG C^GGCCG
874 CGGCCG
Eco81I 1547 CCTGAGG CC^T[ACGT]AGG
1989 CCTGAGG
Ksp632I 672 CTCTTC CTCTTC(1/4)
2614 CTCTTC
MflI 197 GGATCT [GA]^GATC[CT]
3739 GGATCT
NspI 820 ACATGC [GA]CATG^[CT]
3520 ACATGT
PpuMI 514 AGGACCC [GA]G^G[AT]CC[CT]
2310 AGGTCCC
ScaI 346 AGTACT AGT^ACT
376 AGTACT
SmlI 2487 CTCGAG C^T[CT][GA]AG
3822 CTTAAG
XhoII 197 GGATCT [GA]^GATC[CT]
3739 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BcnI BfaI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PmaCI PmlI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI