##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AclI AflIII AscI
AseI AsnI Asp718I BamHI BbeI
BbvCI BciVI BglII BsaBI BseAI
BsiWI BspDI BspEI BspLU11I BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI EcoRI EcoT22I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AatII 168 GACGTC GACGT^C
AccI 3498 GTAGAC GT^[AC][GT]AC
AcsI 566 GAATTT [GA]^AATT[CT]
AflII 4277 CTTAAG C^TTAAG
AgeI 3469 ACCGGT A^CCGGT
AhdI 3303 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3290 GTGCAC G^TGCAC
Aor51HI 1014 AGCGCT AGC^GCT
ApaLI 3290 GTGCAC G^TGCAC
ApoI 566 GAATTT [GA]^AATT[CT]
AspEI 3303 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 1293 TGATCA T^GATCA
BfrI 4277 CTTAAG C^TTAAG
BsrDI 1033 CATTGC GCAATG(2/0)
DraIII 4179 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3303 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1014 AGCGCT AGC^GCT
EcoRV 1327 GATATC GAT^ATC
FbaI 1293 TGATCA T^GATCA
FseI 3619 GGCCGGCC GGCCGG^CC
PinAI 3469 ACCGGT A^CCGGT
PshAI 638 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3355 GCTCTTC GCTCTTC(1/4)
SexAI 1791 ACCTGGT A^CC[AT]GGT
SmlI 4277 CTTAAG C^T[CT][GA]AG
SspI 4448 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 168 GACGTC G[GA]^CG[CT]C
2408 GACGCC
AocI 2770 CCTGAGG CC^T[ACGT]AGG
3261 CCTCAGG
Asp700I 3308 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4447 GAATATTTTC
AspI 1645 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3088 GACAGTGTC
AviII 135 TGCGCA TGC^GCA
1992 TGCGCA
AvrII 3503 CCTAGG C^CTAGG
4020 CCTAGG
BbrPI 558 CACGTG CAC^GTG
1762 CACGTG
BglI 29 GCCAACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2193 GCCTGAACGGC
BlnI 3503 CCTAGG C^CTAGG
4020 CCTAGG
BsaHI 168 GACGTC G[GA]^CG[CT]C
2408 GACGCC
BsaWI 533 ACCGGA [AT]^CCGG[AT]
3469 ACCGGT
BsmI 1513 GAATGC GAATGC(1/-1)
112 GCATTC
BspHI 3773 TCATGA T^CATGA
4244 TCATGA
BspMI 84 ACCTGC ACCTGC(4/8)
2109 ACCTGC
BsrBI 1531 CCGCTC CCGCTC(-3/-3)
1603 CCGCTC
BssSI 3285 CACGAG CACGAG(-5/-1)
3723 CTCGTG
BstEII 2275 GGTCACC G^GT[ACGT]ACC
2571 GGTTACC
BstPI 2275 GGTCACC G^GT[ACGT]ACC
2571 GGTTACC
Bsu36I 2770 CCTGAGG CC^T[ACGT]AGG
3261 CCTCAGG
DrdI 351 GACGCTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4088 GACAGCAAGGTC
EagI 2425 CGGCCG C^GGCCG
3755 CGGCCG
EclXI 2425 CGGCCG C^GGCCG
3755 CGGCCG
Eco52I 2425 CGGCCG C^GGCCG
3755 CGGCCG
Eco81I 2770 CCTGAGG CC^T[ACGT]AGG
3261 CCTCAGG
EcoO65I 2275 GGTCACC G^GT[ACGT]ACC
2571 GGTTACC
FspI 135 TGCGCA TGC^GCA
1992 TGCGCA
Hin1I 168 GACGTC G[GA]^CG[CT]C
2408 GACGCC
HincII 1094 GTCAAC GT[CT]^[GA]AC
1841 GTTGAC
HindII 1094 GTCAAC GT[CT]^[GA]AC
1841 GTTGAC
NaeI 3620 GCCGGC GCC^GGC
3757 GCCGGC
NgoMIV 3620 GCCGGC G^CCGGC
3757 GCCGGC
PflFI 1645 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3088 GACAGTGTC
PmaCI 558 CACGTG CAC^GTG
1762 CACGTG
PmlI 558 CACGTG CAC^GTG
1762 CACGTG
RcaI 3773 TCATGA T^CATGA
4244 TCATGA
SfiI 28 GGCCAACTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2192 GGCCTGAACGGCC
StuI 11 AGGCCT AGG^CCT
4266 AGGCCT
TfiI 3553 GATTC G^A[AT]TC
4248 GAATC
Tsp509I 567 AATT ^AATT
4441 AATT
Tth111I 1645 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3088 GACAGTGTC
XmnI 3308 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4447 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SphI SspBI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI