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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI AspEI
AviII BamHI BbeI BcgI BciVI
BfrI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BstBI BstEII BstPI BstUI Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FseI FspI HgaI Hin1I HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Asp700I 375 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3290 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 900 CACGTG CAC^GTG
BbsI 506 GAAGAC GAAGAC(2/6)
BbvCI 3031 GCTGAGG CCTCAGC(-5/-2)
BglII 2839 AGATCT A^GATCT
BlpI 124 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 124 GCTCAGC GC^T[ACGT]AGC
BpuAI 506 GAAGAC GAAGAC(2/6)
BsaAI 900 CACGTG [CT]AC^GT[GA]
BsmI 3540 GAATGC GAATGC(1/-1)
BspDI 1037 ATCGAT AT^CGAT
BssSI 841 CACGAG CACGAG(-5/-1)
Bst1107I 1878 GTATAC GTA^TAC
BstAPI 4141 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1878 GTATAC GTA^TAC
CelII 124 GCTCAGC GC^T[ACGT]AGC
ClaI 1037 ATCGAT AT^CGAT
DraIII 3313 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 2877 ATGCAT ATGCA^T
HaeII 2013 GGCGCT [GA]GCGC^[CT]
NheI 3301 GCTAGC G^CTAGC
NsiI 2877 ATGCAT ATGCA^T
PaeR7I 460 CTCGAG C^TCGAG
PflFI 3290 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 900 CACGTG CAC^GTG
PmlI 900 CACGTG CAC^GTG
SapI 3755 GAAGAGC GCTCTTC(1/4)
SbfI 3936 CCTGCAGG CCTGCA^GG
ScaI 173 AGTACT AGT^ACT
SmaI 3921 CCCGGG CCC^GGG
Sse8387I 3936 CCTGCAGG CCTGCA^GG
SspI 4412 AATATT AAT^ATT
Tth111I 3290 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 684 TCTAGA T^CTAGA
XcmI 4302 CCAGGCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 460 CTCGAG C^TCGAG
XmaCI 3921 CCCGGG C^CCGGG
XmaI 3921 CCCGGG C^CCGGG
XmnI 375 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 940 GGTACC G^GTACC
1236 GGTACC
AccI 1878 GTATAC GT^[AC][GT]AC
4378 GTCTAC
AflIII 899 ACACGT A^C[GA][CT]GT
901 ACGTGT
Alw44I 34 GTGCAC G^TGCAC
3163 GTGCAC
ApaLI 34 GTGCAC G^TGCAC
3163 GTGCAC
Asp718I 940 GGTACC G^GTACC
1236 GGTACC
BclI 26 TGATCA T^GATCA
1343 TGATCA
BspMI 2955 GCAGGT ACCTGC(4/8)
4306 GCAGGT
CfoI 2014 GCGC GCG^C
2286 GCGC
DrdI 619 GACGAGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3197 GACAAGGAAGTC
FbaI 26 TGATCA T^GATCA
1343 TGATCA
HhaI 2014 GCGC GCG^C
2286 GCGC
HinP1I 2014 GCGC G^CGC
2286 GCGC
HincII 1836 GTCAAC GT[CT]^[GA]AC
2305 GTCAAC
HindII 1836 GTCAAC GT[CT]^[GA]AC
2305 GTCAAC
KpnI 940 GGTACC GGTAC^C
1236 GGTACC
MaeII 901 ACGT A^CGT
1179 ACGT
NspI 2879 GCATGT [GA]CATG^[CT]
4150 GCATGT
StuI 1666 AGGCCT AGG^CCT
2573 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
AvrII BalI BanI BanII BbvI
BcnI BfaI BglI BlnI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII