##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AspEI AviII
BcgI BfrI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BstBI ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FseI FspI HpaI
MamI MfeI MroI MunI NdeI
NheI NotI NruI NspV PacI
PinAI Psp1406I PvuI PvuII RcaI
RsrII SalI ScaI SfuI SmaI
SnaBI SspBI SspI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 4286 GGTACC G^GTACC
AccI 1182 GTATAC GT^[AC][GT]AC
Alw44I 753 GTGCAC G^TGCAC
ApaLI 753 GTGCAC G^TGCAC
AseI 1140 ATTAAT AT^TAAT
AsnI 1140 ATTAAT AT^TAAT
Asp700I 1092 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4286 GGTACC G^GTACC
AspI 2641 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2840 GGATCC G^GATCC
BclI 4298 TGATCA T^GATCA
BglII 4334 AGATCT A^GATCT
BlpI 4263 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4263 GCTGAGC GC^T[ACGT]AGC
Bst1107I 1182 GTATAC GTA^TAC
BstEII 2448 GGTCACC G^GT[ACGT]ACC
BstPI 2448 GGTCACC G^GT[ACGT]ACC
BstZ17I 1182 GTATAC GTA^TAC
CelII 4263 GCTGAGC GC^T[ACGT]AGC
DraIII 747 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6 GACCATGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2448 GGTCACC G^GT[ACGT]ACC
EcoRI 3696 GAATTC G^AATTC
EcoT22I 891 ATGCAT ATGCA^T
FbaI 4298 TGATCA T^GATCA
KpnI 4286 GGTACC GGTAC^C
MluI 2265 ACGCGT A^CGCGT
NaeI 171 GCCGGC GCC^GGC
NgoMIV 171 GCCGGC G^CCGGC
NsiI 891 ATGCAT ATGCA^T
PaeR7I 2656 CTCGAG C^TCGAG
PflFI 2641 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 3575 GTTTAAAC GTTT^AAAC
PshAI 1858 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1140 ATTAAT AT^TAAT
SapI 3715 GCTCTTC GCTCTTC(1/4)
SbfI 2304 CCTGCAGG CCTGCA^GG
SgrAI 170 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 2304 CCTGCAGG CCTGCA^GG
TaqI 2657 TCGA T^CGA
Tth111I 2641 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2657 TCGA T^CGA
XhoI 2656 CTCGAG C^TCGAG
XmnI 1092 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 1092 GAATTT [GA]^AATT[CT]
3696 GAATTC
AflIII 899 ACGTGT A^C[GA][CT]GT
2265 ACGCGT
ApaI 1806 GGGCCC GGGCC^C
2395 GGGCCC
ApoI 1092 GAATTT [GA]^AATT[CT]
3696 GAATTC
AvrII 279 CCTAGG C^CTAGG
1384 CCTAGG
BbsI 800 GAAGAC GAAGAC(2/6)
3821 GAAGAC
BbvCI 3353 CCTCAGC CCTCAGC(-5/-2)
661 GCTGAGG
BciVI 1564 GGATAC GTATCC(6/5)
903 GTATCC
BlnI 279 CCTAGG C^CTAGG
1384 CCTAGG
BpuAI 800 GAAGAC GAAGAC(2/6)
3821 GAAGAC
BsaI 1774 GAGACC GGTCTC(1/5)
2524 GGTCTC
BsiEI 1553 CGGTCG CG[GA][CT]^CG
2640 CGACCG
BsmBI 176 CGTCTC CGTCTC(1/5)
516 GAGACG
BsmI 1217 GAATGC GAATGC(1/-1)
1611 GCATTC
BspMI 417 ACCTGC ACCTGC(4/8)
2303 ACCTGC
BsrDI 3398 GCAATG GCAATG(2/0)
3998 GCAATG
BstAPI 873 GCACAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1324 GCAGGCACTGC
BstXI 3549 CCAGAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3890 CCAGGACTCTGG
BstYI 2840 GGATCC [GA]^GATC[CT]
4334 AGATCT
DraI 3576 TTTAAA TTT^AAA
3594 TTTAAA
HincII 1760 GTTGAC GT[CT]^[GA]AC
2718 GTCAAC
HindII 1760 GTTGAC GT[CT]^[GA]AC
2718 GTCAAC
HindIII 2859 AAGCTT A^AGCTT
3784 AAGCTT
KspI 2649 CCGCGG CCGC^GG
2973 CCGCGG
MflI 2840 GGATCC [GA]^GATC[CT]
4334 AGATCT
SacII 2649 CCGCGG CCGC^GG
2973 CCGCGG
SexAI 1542 ACCAGGT A^CC[AT]GGT
1701 ACCAGGT
SfiI 693 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1807 GGCCCCCAGGGCC
SmlI 2212 CTTGAG C^T[CT][GA]AG
2656 CTCGAG
SpeI 1204 ACTAGT A^CTAGT
2957 ACTAGT
SphI 893 GCATGC GCATG^C
3260 GCATGC
TfiI 1008 GAATC G^A[AT]TC
3954 GAATC
XhoII 2840 GGATCC [GA]^GATC[CT]
4334 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StuI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI