##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII BbrPI BclI BsaAI BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 965 GTAGAC GT^[AC][GT]AC
AccII 325 CGCG CG^CG
AflII 4658 CTTAAG C^TTAAG
AflIII 284 ACATGT A^C[GA][CT]GT
Alw44I 1159 GTGCAC G^TGCAC
ApaLI 1159 GTGCAC G^TGCAC
AspI 2603 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 293 TGGCCA TGG^CCA
BamHI 426 GGATCC G^GATCC
BcgI 4628 GCACCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1133 GTATCC GTATCC(6/5)
BfrI 4658 CTTAAG C^TTAAG
BsaWI 1872 TCCGGT [AT]^CCGG[AT]
BsgI 3430 GTGCAG GTGCAG(16/14)
BsmBI 2266 CGTCTC CGTCTC(1/5)
Bsp1407I 1178 TGTACA T^GTACA
BspHI 1833 TCATGA T^CATGA
BspLU11I 284 ACATGT A^CATGT
BsrGI 1178 TGTACA T^GTACA
BstUI 325 CGCG CG^CG
DraIII 223 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3758 GACATGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 392 GAATTC G^AATTC
HindIII 3592 AAGCTT A^AGCTT
MaeII 677 ACGT A^CGT
MluNI 293 TGGCCA TGG^CCA
MscI 293 TGGCCA TGG^CCA
MvnI 325 CGCG CG^CG
NdeI 901 CATATG CA^TATG
PaeR7I 659 CTCGAG C^TCGAG
PflFI 2603 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1833 TCATGA T^CATGA
SgrAI 1141 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 2702 ACTAGT A^CTAGT
SphI 1626 GCATGC GCATG^C
SspBI 1178 TGTACA T^GTACA
TaqI 660 TCGA T^CGA
TfiI 4050 GAATC G^A[AT]TC
Tth111I 2603 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 660 TCGA T^CGA
XhoI 659 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1145 GGCGCC G[GA]^CG[CT]C
1484 GGCGCC
AhdI 2868 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4613 GACCGTAGGTC
Asp700I 3462 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4056 GAACCACTTC
AspEI 2868 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4613 GACCGTAGGTC
AvrII 2255 CCTAGG C^CTAGG
3615 CCTAGG
BbeI 1145 GGCGCC GGCGC^C
1484 GGCGCC
BglII 3696 AGATCT A^GATCT
3822 AGATCT
BlnI 2255 CCTAGG C^CTAGG
3615 CCTAGG
BsaHI 1145 GGCGCC G[GA]^CG[CT]C
1484 GGCGCC
BsmI 3398 GAATGC GAATGC(1/-1)
4522 GAATGC
BspMI 780 ACCTGC ACCTGC(4/8)
1087 ACCTGC
BstAPI 1614 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4393 GCAGTGGCTGC
Eam1105I 2868 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4613 GACCGTAGGTC
FauI 1568 CCCGC CCCGC(4/6)
1755 GCGGG
Hin1I 1145 GGCGCC G[GA]^CG[CT]C
1484 GGCGCC
HincII 1856 GTCAAC GT[CT]^[GA]AC
3629 GTCAAC
HindII 1856 GTCAAC GT[CT]^[GA]AC
3629 GTCAAC
KasI 1145 GGCGCC G^GCGCC
1484 GGCGCC
NaeI 577 GCCGGC GCC^GGC
1142 GCCGGC
NarI 1145 GGCGCC GG^CGCC
1484 GGCGCC
NcoI 995 CCATGG C^CATGG
4501 CCATGG
NgoMIV 577 GCCGGC G^CCGGC
1142 GCCGGC
PshAI 1339 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3078 GACTTGAGTC
SapI 4474 GAAGAGC GCTCTTC(1/4)
4330 GCTCTTC
SexAI 815 ACCAGGT A^CC[AT]GGT
4292 ACCAGGT
SfoI 1145 GGCGCC GGC^GCC
1484 GGCGCC
SspI 2763 AATATT AAT^ATT
2783 AATATT
StuI 603 AGGCCT AGG^CCT
1958 AGGCCT
XbaI 2584 TCTAGA T^CTAGA
4468 TCTAGA
XmnI 3462 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4056 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII XmaCI XmaI