##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI Asp718I AspEI AviII
AvrII BamHI BcgI BglII BlnI
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoT22I FseI
FspI HincII HindII HpaI KpnI
KspI MamI MluI MroI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3706 GTCTAC GT^[AC][GT]AC
AflII 4257 CTTAAG C^TTAAG
Aor51HI 1143 AGCGCT AGC^GCT
ApaI 2826 GGGCCC GGGCC^C
AseI 2501 ATTAAT AT^TAAT
AsnI 2501 ATTAAT AT^TAAT
BfrI 4257 CTTAAG C^TTAAG
BsiEI 1279 CGACCG CG[GA][CT]^CG
BspLU11I 1032 ACATGT A^CATGT
BsrFI 4207 GCCGGC [GA]^CCGG[CT]
BssSI 905 CACGAG CACGAG(-5/-1)
Cfr10I 4207 GCCGGC [GA]^CCGG[CT]
DraI 2539 TTTAAA TTT^AAA
Eco47III 1143 AGCGCT AGC^GCT
EcoNI 3241 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4548 GATATC GAT^ATC
HindIII 387 AAGCTT A^AGCTT
MfeI 4437 CAATTG C^AATTG
MunI 4437 CAATTG C^AATTG
NaeI 4207 GCCGGC GCC^GGC
NcoI 26 CCATGG C^CATGG
NdeI 2996 CATATG CA^TATG
NgoMIV 4207 GCCGGC G^CCGGC
PshBI 2501 ATTAAT AT^TAAT
SacI 3856 GAGCTC GAGCT^C
SspI 1707 AATATT AAT^ATT
XbaI 4167 TCTAGA T^CTAGA
XcmI 1722 CCAGCCCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 1032 ACATGT A^C[GA][CT]GT
1431 ACACGT
Alw44I 76 GTGCAC G^TGCAC
1343 GTGCAC
AocI 2123 CCTTAGG CC^T[ACGT]AGG
2911 CCTCAGG
ApaLI 76 GTGCAC G^TGCAC
1343 GTGCAC
Asp700I 1393 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3458 GAAAAAATTC
BbrPI 1070 CACGTG CAC^GTG
1357 CACGTG
BbsI 1363 GAAGAC GAAGAC(2/6)
3689 GTCTTC
BciVI 3132 GTATCC GTATCC(6/5)
3165 GTATCC
BclI 1950 TGATCA T^GATCA
4592 TGATCA
BglI 1154 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3864 GCCTTGCGGGC
BpuAI 1363 GAAGAC GAAGAC(2/6)
3689 GTCTTC
BsmBI 4280 CGTCTC CGTCTC(1/5)
939 GAGACG
Bsp1407I 53 TGTACA T^GTACA
1035 TGTACA
BsrDI 2562 CATTGC GCAATG(2/0)
2738 GCAATG
BsrGI 53 TGTACA T^GTACA
1035 TGTACA
BstAPI 1679 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1957 GCAGGCTGTGC
BstEII 564 GGTGACC G^GT[ACGT]ACC
1752 GGTCACC
BstPI 564 GGTGACC G^GT[ACGT]ACC
1752 GGTCACC
BstYI 33 GGATCT [GA]^GATC[CT]
948 GGATCT
Bsu36I 2123 CCTTAGG CC^T[ACGT]AGG
2911 CCTCAGG
Eco81I 2123 CCTTAGG CC^T[ACGT]AGG
2911 CCTCAGG
EcoO65I 564 GGTGACC G^GT[ACGT]ACC
1752 GGTCACC
FbaI 1950 TGATCA T^GATCA
4592 TGATCA
HgaI 1490 GACGC GACGC(5/10)
3947 GCGTC
MflI 33 GGATCT [GA]^GATC[CT]
948 GGATCT
NspI 1032 ACATGT [GA]CATG^[CT]
4139 ACATGC
PaeR7I 936 CTCGAG C^TCGAG
2390 CTCGAG
PmaCI 1070 CACGTG CAC^GTG
1357 CACGTG
PmlI 1070 CACGTG CAC^GTG
1357 CACGTG
PpuMI 691 AGGTCCT [GA]G^G[AT]CC[CT]
924 AGGACCC
SspBI 53 TGTACA T^GTACA
1035 TGTACA
XhoI 936 CTCGAG C^TCGAG
2390 CTCGAG
XhoII 33 GGATCT [GA]^GATC[CT]
948 GGATCT
XmnI 1393 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3458 GAAAAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PflFI PflMI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XmaCI XmaI