##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AscI Asp718I AspI AvrII
BbeI BglII BlnI BseAI BsiEI
BsiWI BspEI BspHI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CpoI DrdI EagI
EclXI Eco52I EcoO65I EcoT22I FauI
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 1266 GACGCC G[GA]^CG[CT]C
AgeI 3027 ACCGGT A^CCGGT
AhdI 2138 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1231 GTGCAC G^TGCAC
Aor51HI 25 AGCGCT AGC^GCT
ApaLI 1231 GTGCAC G^TGCAC
Asp700I 3726 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2138 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 989 CCCGAG C^[CT]CG[GA]G
AviII 3646 TGCGCA TGC^GCA
BamHI 1855 GGATCC G^GATCC
BbrPI 2715 CACGTG CAC^GTG
BbvCI 1682 CCTCAGC CCTCAGC(-5/-2)
BcgI 290 CGACCCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2715 CACGTG [CT]AC^GT[GA]
BsaBI 494 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1266 GACGCC G[GA]^CG[CT]C
BsmBI 3779 GAGACG CGTCTC(1/5)
BsoBI 989 CCCGAG C^[CT]CG[GA]G
BspDI 3207 ATCGAT AT^CGAT
ClaI 3207 ATCGAT AT^CGAT
DraIII 3316 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2138 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 25 AGCGCT AGC^GCT
EcoRI 3667 GAATTC G^AATTC
EcoRV 207 GATATC GAT^ATC
FspI 3646 TGCGCA TGC^GCA
HaeII 25 AGCGCT [GA]GCGC^[CT]
Hin1I 1266 GACGCC G[GA]^CG[CT]C
MamI 494 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2117 CCATGG C^CATGG
PflMI 526 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3027 ACCGGT A^CCGGT
PmaCI 2715 CACGTG CAC^GTG
PmeI 1073 GTTTAAAC GTTT^AAAC
PmlI 2715 CACGTG CAC^GTG
PshAI 2239 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 2645 AGTACT AGT^ACT
SphI 2709 GCATGC GCATG^C
SwaI 4292 ATTTAAAT ATTT^AAAT
Van91I 526 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3726 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 1817 CTTAAG C^TTAAG
3912 CTTAAG
AflIII 1739 ACATGT A^C[GA][CT]GT
3005 ACATGT
AlwNI 911 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
3576 CAGAGCCTG
AseI 3891 ATTAAT AT^TAAT
4090 ATTAAT
AsnI 3891 ATTAAT AT^TAAT
4090 ATTAAT
BanI 1318 GGTGCC G^G[CT][GA]CC
2921 GGCACC
BclI 406 TGATCA T^GATCA
1752 TGATCA
BfrI 1817 CTTAAG C^TTAAG
3912 CTTAAG
BglI 356 GCCCGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
986 GCCCCCGAGGC
BlpI 958 GCTCAGC GC^T[ACGT]AGC
1919 GCTCAGC
Bpu1102I 958 GCTCAGC GC^T[ACGT]AGC
1919 GCTCAGC
BsaI 1087 GAGACC GGTCTC(1/5)
2347 GAGACC
BsaWI 188 ACCGGA [AT]^CCGG[AT]
3027 ACCGGT
BsmI 2557 GCATTC GAATGC(1/-1)
3128 GCATTC
Bsp1407I 1597 TGTACA T^GTACA
3762 TGTACA
BspLU11I 1739 ACATGT A^CATGT
3005 ACATGT
BspMI 1731 ACCTGC ACCTGC(4/8)
4195 ACCTGC
BsrFI 972 ACCGGC [GA]^CCGG[CT]
3027 ACCGGT
BsrGI 1597 TGTACA T^GTACA
3762 TGTACA
BstAPI 2922 GCACCATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3093 GCACTGACTGC
BstXI 499 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1383 CCAGTAAGCTGG
CelII 958 GCTCAGC GC^T[ACGT]AGC
1919 GCTCAGC
CfoI 26 GCGC GCG^C
3647 GCGC
Cfr10I 972 ACCGGC [GA]^CCGG[CT]
3027 ACCGGT
DsaI 358 CCGTGG C^C[GA][CT]GG
2117 CCATGG
EcoNI 719 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2620 CCTGGCCAAGG
FbaI 406 TGATCA T^GATCA
1752 TGATCA
HgaI 195 GACGC GACGC(5/10)
1266 GACGC
HhaI 26 GCGC GCG^C
3647 GCGC
HinP1I 26 GCGC G^CGC
3647 GCGC
NdeI 2926 CATATG CA^TATG
3660 CATATG
PshBI 3891 ATTAAT AT^TAAT
4090 ATTAAT
SapI 847 GCTCTTC GCTCTTC(1/4)
2062 GCTCTTC
SspBI 1597 TGTACA T^GTACA
3762 TGTACA
StuI 518 AGGCCT AGG^CCT
1124 AGGCCT
XbaI 2842 TCTAGA T^CTAGA
4166 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApaI ApoI AspHI AvaII
BalI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII