##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BstAPI BstBI BstUI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoRV FseI FspI
Hin1I KasI KpnI KspI MamI
MluI MroI MvnI NarI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 3925 CTTAAG C^TTAAG
AocI 746 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1102 AGCGCT AGC^GCT
BciVI 3807 GGATAC GTATCC(6/5)
BclI 3433 TGATCA T^GATCA
BfrI 3925 CTTAAG C^TTAAG
BlpI 584 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 584 GCTAAGC GC^T[ACGT]AGC
BsiEI 995 CGACCG CG[GA][CT]^CG
BsmBI 1741 CGTCTC CGTCTC(1/5)
Bsp1407I 3982 TGTACA T^GTACA
BspMI 1651 GCAGGT ACCTGC(4/8)
BsrDI 2278 CATTGC GCAATG(2/0)
BsrFI 1581 GCCGGC [GA]^CCGG[CT]
BsrGI 3982 TGTACA T^GTACA
BssSI 1362 CACGAG CACGAG(-5/-1)
Bst1107I 4143 GTATAC GTA^TAC
BstZ17I 4143 GTATAC GTA^TAC
Bsu36I 746 CCTCAGG CC^T[ACGT]AGG
CelII 584 GCTAAGC GC^T[ACGT]AGC
CfoI 1103 GCGC GCG^C
Cfr10I 1581 GCCGGC [GA]^CCGG[CT]
Eco47III 1102 AGCGCT AGC^GCT
Eco81I 746 CCTCAGG CC^T[ACGT]AGG
FbaI 3433 TGATCA T^GATCA
HaeII 1102 AGCGCT [GA]GCGC^[CT]
HhaI 1103 GCGC GCG^C
HinP1I 1103 GCGC G^CGC
HindIII 35 AAGCTT A^AGCTT
HpaI 3380 GTTAAC GTT^AAC
MaeII 697 ACGT A^CGT
NaeI 1581 GCCGGC GCC^GGC
NdeI 4219 CATATG CA^TATG
NgoMIV 1581 GCCGGC G^CCGGC
SalI 711 GTCGAC G^TCGAC
SexAI 982 ACCTGGT A^CC[AT]GGT
SmaI 1758 CCCGGG CCC^GGG
SspBI 3982 TGTACA T^GTACA
StuI 955 AGGCCT AGG^CCT
XbaI 629 TCTAGA T^CTAGA
XmaCI 1758 CCCGGG C^CCGGG
XmaI 1758 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 1341 GTGCAC G^TGCAC
1400 GTGCAC
ApaLI 1341 GTGCAC G^TGCAC
1400 GTGCAC
AseI 3677 ATTAAT AT^TAAT
4485 ATTAAT
AsnI 3677 ATTAAT AT^TAAT
4485 ATTAAT
AvaI 529 CCCGAG C^[CT]CG[GA]G
1758 CCCGGG
BcgI 1990 CGATAGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1402 GCACTCTCCTCG
BglII 1139 AGATCT A^GATCT
3734 AGATCT
BsaI 4361 GAGACC GGTCTC(1/5)
2358 GGTCTC
BsoBI 529 CCCGAG C^[CT]CG[GA]G
1758 CCCGGG
BspHI 1888 TCATGA T^CATGA
3619 TCATGA
BsrBI 998 CCGCTC CCGCTC(-3/-3)
3862 CCGCTC
DraI 2648 TTTAAA TTT^AAA
3642 TTTAAA
DraIII 981 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3781 CACAGCGTG
EcoRI 2372 GAATTC G^AATTC
4033 GAATTC
FauI 3861 CCCGC CCCGC(4/6)
1672 GCGGG
HgaI 714 GACGC GACGC(5/10)
1816 GCGTC
MfeI 2152 CAATTG C^AATTG
4247 CAATTG
MunI 2152 CAATTG C^AATTG
4247 CAATTG
NspI 1036 ACATGC [GA]CATG^[CT]
4171 ACATGC
PshAI 2701 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2878 GACTGGTGTC
PshBI 3677 ATTAAT AT^TAAT
4485 ATTAAT
PvuII 2056 CAGCTG CAG^CTG
2827 CAGCTG
RcaI 1888 TCATGA T^CATGA
3619 TCATGA
SacI 2598 GAGCTC GAGCT^C
4294 GAGCTC
ScaI 2998 AGTACT AGT^ACT
4016 AGTACT
SmlI 1614 CTCAAG C^T[CT][GA]AG
3925 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI PflMI
PleI PpuMI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI