##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BcgI BciVI
BclI BfrI BglII BlnI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BsrGI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI HpaI KpnI
KspI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 852 GACGTC GACGT^C
AccI 2499 GTCGAC GT^[AC][GT]AC
AccII 833 CGCG CG^CG
AfaI 1518 GTAC GT^AC
AflIII 4024 ACATGT A^C[GA][CT]GT
AviII 3533 TGCGCA TGC^GCA
BbeI 2682 GGCGCC GGCGC^C
BbrPI 1292 CACGTG CAC^GTG
BsaAI 1292 CACGTG [CT]AC^GT[GA]
BsaBI 1604 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2279 CGGCCG CG[GA][CT]^CG
BsmBI 4167 GAGACG CGTCTC(1/5)
BspLU11I 4024 ACATGT A^CATGT
BssHII 832 GCGCGC G^CGCGC
BstEII 944 GGTGACC G^GT[ACGT]ACC
BstPI 944 GGTGACC G^GT[ACGT]ACC
BstUI 833 CGCG CG^CG
EagI 2279 CGGCCG C^GGCCG
EclXI 2279 CGGCCG C^GGCCG
Eco52I 2279 CGGCCG C^GGCCG
EcoO65I 944 GGTGACC G^GT[ACGT]ACC
FspI 3533 TGCGCA TGC^GCA
KasI 2682 GGCGCC G^GCGCC
MamI 1604 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 833 CGCG CG^CG
NarI 2682 GGCGCC GG^CGCC
NheI 1560 GCTAGC G^CTAGC
PmaCI 1292 CACGTG CAC^GTG
PmlI 1292 CACGTG CAC^GTG
RsaI 1518 GTAC GT^AC
SalI 2499 GTCGAC G^TCGAC
SapI 3455 GCTCTTC GCTCTTC(1/4)
SexAI 2494 ACCTGGT A^CC[AT]GGT
SfiI 134 GGCCCCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2682 GGCGCC GGC^GCC
SphI 3100 GCATGC GCATG^C
Tsp509I 3056 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 852 GACGTC G[GA]^CG[CT]C
2682 GGCGCC
AocI 221 CCTCAGG CC^T[ACGT]AGG
2309 CCTCAGG
BbsI 3944 GAAGAC GAAGAC(2/6)
2952 GTCTTC
BpuAI 3944 GAAGAC GAAGAC(2/6)
2952 GTCTTC
BsaHI 852 GACGTC G[GA]^CG[CT]C
2682 GGCGCC
BsrBI 3686 CCGCTC CCGCTC(-3/-3)
632 GAGCGG
Bsu36I 221 CCTCAGG CC^T[ACGT]AGG
2309 CCTCAGG
Eco81I 221 CCTCAGG CC^T[ACGT]AGG
2309 CCTCAGG
Hin1I 852 GACGTC G[GA]^CG[CT]C
2682 GGCGCC
MseI 2977 TTAA T^TAA
3006 TTAA
SmaI 1087 CCCGGG CCC^GGG
2172 CCCGGG
SmlI 432 CTCAAG C^T[CT][GA]AG
3068 CTTGAG
StuI 919 AGGCCT AGG^CCT
3437 AGGCCT
Tru9I 2977 TTAA T^TAA
3006 TTAA
XcmI 2043 CCAGCTCCATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3094 CCAAGAGCATGCTGG
XmaCI 1087 CCCGGG C^CCGGG
2172 CCCGGG
XmaI 1087 CCCGGG C^CCGGG
2172 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XhoII