##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII BclI BfrI
BsaBI BsiWI BspDI BspLU11I BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2138 GACGTC GACGT^C
AccIII 1238 TCCGGA T^CCGGA
Alw44I 1354 GTGCAC G^TGCAC
ApaLI 1354 GTGCAC G^TGCAC
Asp700I 4415 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1225 CCTAGG C^CTAGG
BamHI 322 GGATCC G^GATCC
BbeI 1881 GGCGCC GGCGC^C
BbsI 1151 GTCTTC GAAGAC(2/6)
BcgI 335 GCACATGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3979 AGATCT A^GATCT
BlnI 1225 CCTAGG C^CTAGG
BpuAI 1151 GTCTTC GAAGAC(2/6)
BsaWI 1238 TCCGGA [AT]^CCGG[AT]
BseAI 1238 TCCGGA T^CCGGA
BsmBI 1733 GAGACG CGTCTC(1/5)
Bsp1407I 2995 TGTACA T^GTACA
BspEI 1238 TCCGGA T^CCGGA
BspHI 454 TCATGA T^CATGA
BsrGI 2995 TGTACA T^GTACA
EcoRI 1685 GAATTC G^AATTC
HindIII 4076 AAGCTT A^AGCTT
KasI 1881 GGCGCC G^GCGCC
MroI 1238 TCCGGA T^CCGGA
MseI 4339 TTAA T^TAA
NarI 1881 GGCGCC GG^CGCC
NdeI 4166 CATATG CA^TATG
PaeR7I 1792 CTCGAG C^TCGAG
RcaI 454 TCATGA T^CATGA
SacI 1395 GAGCTC GAGCT^C
ScaI 1749 AGTACT AGT^ACT
SfoI 1881 GGCGCC GGC^GCC
SmlI 1792 CTCGAG C^T[CT][GA]AG
SspBI 2995 TGTACA T^GTACA
Tru9I 4339 TTAA T^TAA
XhoI 1792 CTCGAG C^TCGAG
XmnI 4415 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1037 GTCTAC GT^[AC][GT]AC
4239 GTAGAC
AspI 114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1558 GACCTTGTC
BciVI 1515 GGATAC GTATCC(6/5)
4083 GTATCC
BsiEI 1388 CGGCCG CG[GA][CT]^CG
2435 CGGCCG
BsrBI 6 CCGCTC CCGCTC(-3/-3)
2179 GAGCGG
DraIII 892 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2811 CACGCAGTG
EagI 1388 CGGCCG C^GGCCG
2435 CGGCCG
EarI 2937 GAAGAG CTCTTC(1/4)
4201 GAAGAG
EclXI 1388 CGGCCG C^GGCCG
2435 CGGCCG
Eco52I 1388 CGGCCG C^GGCCG
2435 CGGCCG
HincII 3685 GTTGAC GT[CT]^[GA]AC
4148 GTTGAC
HindII 3685 GTTGAC GT[CT]^[GA]AC
4148 GTTGAC
Ksp632I 2937 GAAGAG CTCTTC(1/4)
4201 GAAGAG
NaeI 2440 GCCGGC GCC^GGC
3390 GCCGGC
NcoI 3236 CCATGG C^CATGG
3907 CCATGG
NgoMIV 2440 GCCGGC G^CCGGC
3390 GCCGGC
NspI 511 ACATGC [GA]CATG^[CT]
2624 GCATGT
PflFI 114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1558 GACCTTGTC
SbfI 1467 CCTGCAGG CCTGCA^GG
1566 CCTGCAGG
Sse8387I 1467 CCTGCAGG CCTGCA^GG
1566 CCTGCAGG
Tth111I 114 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1558 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI