##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AcsI AflIII Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AvrII BamHI BciVI
BclI BlnI BsiWI BspDI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I ClaI DraI DrdI Eco47III
EcoRI FbaI HindIII HpaI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SalI SbfI SexAI SfuI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3252 GACGTC GACGT^C
Acc65I 899 GGTACC G^GTACC
AflII 1821 CTTAAG C^TTAAG
AhdI 1974 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 899 GGTACC G^GTACC
AspEI 1974 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 414 TGCGCA TGC^GCA
BbeI 974 GGCGCC GGCGC^C
BbrPI 2856 CACGTG CAC^GTG
BcgI 1591 GCATGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1821 CTTAAG C^TTAAG
BglII 2554 AGATCT A^GATCT
BsaBI 687 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1695 GAGACG CGTCTC(1/5)
BsmI 59 GAATGC GAATGC(1/-1)
Bsp1407I 4355 TGTACA T^GTACA
BsrGI 4355 TGTACA T^GTACA
BstEII 505 GGTAACC G^GT[ACGT]ACC
BstPI 505 GGTAACC G^GT[ACGT]ACC
CpoI 309 CGGTCCG CG^G[AT]CCG
DraIII 3345 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1974 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 505 GGTAACC G^GT[ACGT]ACC
EcoRV 5 GATATC GAT^ATC
EcoT22I 61 ATGCAT ATGCA^T
FspI 414 TGCGCA TGC^GCA
HaeII 974 GGCGCC [GA]GCGC^[CT]
KasI 974 GGCGCC G^GCGCC
KpnI 899 GGTACC GGTAC^C
MamI 687 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 974 GGCGCC GG^CGCC
NcoI 4098 CCATGG C^CATGG
NsiI 61 ATGCAT ATGCA^T
NspI 63 GCATGC [GA]CATG^[CT]
PaeR7I 3956 CTCGAG C^TCGAG
PmaCI 2856 CACGTG CAC^GTG
PmlI 2856 CACGTG CAC^GTG
RsrII 309 CGGTCCG CG^G[AT]CCG
SapI 2987 GAAGAGC GCTCTTC(1/4)
ScaI 1578 AGTACT AGT^ACT
SfiI 2738 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 974 GGCGCC GGC^GCC
SgrAI 3613 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2749 CCCGGG CCC^GGG
SphI 63 GCATGC GCATG^C
SspBI 4355 TGTACA T^GTACA
SspI 4035 AATATT AAT^ATT
XhoI 3956 CTCGAG C^TCGAG
XmaCI 2749 CCCGGG C^CCGGG
XmaI 2749 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AgeI 2200 ACCGGT A^CCGGT
3614 ACCGGT
AlwI 555 GGATC GGATC(4/5)
2914 GGATC
BbsI 2204 GTCTTC GAAGAC(2/6)
2573 GTCTTC
BlpI 2030 GCTCAGC GC^T[ACGT]AGC
2777 GCTGAGC
Bpu1102I 2030 GCTCAGC GC^T[ACGT]AGC
2777 GCTGAGC
BpuAI 2204 GTCTTC GAAGAC(2/6)
2573 GTCTTC
BsaAI 2841 CACGTA [CT]AC^GT[GA]
2856 CACGTG
BsiEI 2445 CGGCCG CG[GA][CT]^CG
3311 CGGCCG
BsrBI 916 GAGCGG CCGCTC(-3/-3)
1060 GAGCGG
BsrDI 1919 CATTGC GCAATG(2/0)
417 GCAATG
BstYI 555 GGATCT [GA]^GATC[CT]
2554 AGATCT
CelII 2030 GCTCAGC GC^T[ACGT]AGC
2777 GCTGAGC
EagI 2445 CGGCCG C^GGCCG
3311 CGGCCG
EclXI 2445 CGGCCG C^GGCCG
3311 CGGCCG
Eco52I 2445 CGGCCG C^GGCCG
3311 CGGCCG
EcoNI 1616 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3776 CCTGGGGGAGG
FseI 3198 GGCCGGCC GGCCGG^CC
3312 GGCCGGCC
HincII 636 GTCAAC GT[CT]^[GA]AC
3333 GTCAAC
HindII 636 GTCAAC GT[CT]^[GA]AC
3333 GTCAAC
KspI 2001 CCGCGG CCGC^GG
3320 CCGCGG
MflI 555 GGATCT [GA]^GATC[CT]
2554 AGATCT
PinAI 2200 ACCGGT A^CCGGT
3614 ACCGGT
SacII 2001 CCGCGG CCGC^GG
3320 CCGCGG
XhoII 555 GGATCT [GA]^GATC[CT]
2554 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AluI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI