##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbeI BciVI BfrI BsaBI
BsiEI BsiWI BspDI BsrFI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRV FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 2818 TCCGGA T^CCGGA
AhdI 1164 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2873 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1164 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 431 CCTAGG C^CTAGG
BbvCI 4159 CCTCAGC CCTCAGC(-5/-2)
BclI 4358 TGATCA T^GATCA
BlnI 431 CCTAGG C^CTAGG
BlpI 2827 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2827 GCTGAGC GC^T[ACGT]AGC
BseAI 2818 TCCGGA T^CCGGA
BsmI 2762 GCATTC GAATGC(1/-1)
BspEI 2818 TCCGGA T^CCGGA
BspHI 4366 TCATGA T^CATGA
BspLU11I 1441 ACATGT A^CATGT
BsrBI 794 GAGCGG CCGCTC(-3/-3)
BstAPI 4121 GCACTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2732 GGTGACC G^GT[ACGT]ACC
BstPI 2732 GGTGACC G^GT[ACGT]ACC
CelII 2827 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1164 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1355 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2732 GGTGACC G^GT[ACGT]ACC
EcoT22I 4664 ATGCAT ATGCA^T
FbaI 4358 TGATCA T^GATCA
MroI 2818 TCCGGA T^CCGGA
NdeI 4454 CATATG CA^TATG
NsiI 4664 ATGCAT ATGCA^T
PshAI 877 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4366 TCATGA T^CATGA
SacI 1014 GAGCTC GAGCT^C
SphI 2703 GCATGC GCATG^C
XmnI 2873 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1044 CGCG CG^CG
2786 CGCG
AcyI 373 GACGCC G[GA]^CG[CT]C
808 GGCGTC
AflIII 1441 ACATGT A^C[GA][CT]GT
2528 ACACGT
ApaI 1909 GGGCCC GGGCC^C
2860 GGGCCC
BbrPI 2529 CACGTG CAC^GTG
4110 CACGTG
BglI 1047 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3886 GCCCCAAAGGC
BsaAI 2529 CACGTG [CT]AC^GT[GA]
4110 CACGTG
BsaHI 373 GACGCC G[GA]^CG[CT]C
808 GGCGTC
BsaWI 1945 ACCGGA [AT]^CCGG[AT]
2818 TCCGGA
Bsp1407I 3262 TGTACA T^GTACA
3950 TGTACA
BspMI 2291 GCAGGT ACCTGC(4/8)
2832 GCAGGT
BsrGI 3262 TGTACA T^GTACA
3950 TGTACA
BssSI 158 CACGAG CACGAG(-5/-1)
2686 CTCGTG
BstUI 1044 CGCG CG^CG
2786 CGCG
DraI 3389 TTTAAA TTT^AAA
4621 TTTAAA
DraIII 2727 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3771 CACATAGTG
EcoRI 3980 GAATTC G^AATTC
4370 GAATTC
Hin1I 373 GACGCC G[GA]^CG[CT]C
808 GGCGTC
MvnI 1044 CGCG CG^CG
2786 CGCG
NcoI 2031 CCATGG C^CATGG
3786 CCATGG
PflMI 414 CCACATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1760 CCATGAACTGG
PmaCI 2529 CACGTG CAC^GTG
4110 CACGTG
PmlI 2529 CACGTG CAC^GTG
4110 CACGTG
SspBI 3262 TGTACA T^GTACA
3950 TGTACA
Van91I 414 CCACATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1760 CCATGAACTGG
XcmI 182 CCAGGACCCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
330 CCAAAACAACTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII