##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AcsI AflII AhdI
ApoI AscI AspEI AvrII BciVI
BclI BfrI BglII BlnI BsaBI
BsiWI BsmI BspHI BspLU11I BsrDI
BstBI CpoI DraIII Eam1105I EcoRI
EcoRV FbaI HindIII HpaI KspI
MamI MfeI MluI MunI NheI
NotI NruI NspV PacI PmeI
PshAI Psp1406I RcaI RsrII SacII
SalI ScaI SfuI SnaBI SpeI
SspI XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 1660 TCCGGA T^CCGGA
AflIII 429 ACACGT A^C[GA][CT]GT
AseI 4497 ATTAAT AT^TAAT
AsnI 4497 ATTAAT AT^TAAT
Asp700I 1924 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 4289 TGCGCA TGC^GCA
BfaI 1754 CTAG C^TAG
BlpI 3311 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3311 GCTCAGC GC^T[ACGT]AGC
BseAI 1660 TCCGGA T^CCGGA
Bsp1407I 2157 TGTACA T^GTACA
BspDI 533 ATCGAT AT^CGAT
BspEI 1660 TCCGGA T^CCGGA
BsrGI 2157 TGTACA T^GTACA
BssHII 596 GCGCGC G^CGCGC
Bst1107I 1543 GTATAC GTA^TAC
BstZ17I 1543 GTATAC GTA^TAC
CelII 3311 GCTCAGC GC^T[ACGT]AGC
ClaI 533 ATCGAT AT^CGAT
DraI 3760 TTTAAA TTT^AAA
DrdI 2975 GACATCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2705 ATGCAT ATGCA^T
FspI 4289 TGCGCA TGC^GCA
MaeI 1754 CTAG C^TAG
MroI 1660 TCCGGA T^CCGGA
NcoI 502 CCATGG C^CATGG
NdeI 2681 CATATG CA^TATG
NsiI 2705 ATGCAT ATGCA^T
PshBI 4497 ATTAAT AT^TAAT
PvuI 1968 CGATCG CGAT^CG
SfiI 785 GGCCGGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1288 CACCGGCG C[GA]^CCGG[CT]G
SphI 2703 GCATGC GCATG^C
SspBI 2157 TGTACA T^GTACA
SwaI 3759 ATTTAAAT ATTT^AAAT
XmnI 1924 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 821 GTCTAC GT^[AC][GT]AC
1543 GTATAC
AgeI 1224 ACCGGT A^CCGGT
2646 ACCGGT
Alw44I 58 GTGCAC G^TGCAC
1622 GTGCAC
Aor51HI 354 AGCGCT AGC^GCT
939 AGCGCT
ApaLI 58 GTGCAC G^TGCAC
1622 GTGCAC
BamHI 836 GGATCC G^GATCC
2283 GGATCC
BbsI 1963 GAAGAC GAAGAC(2/6)
2758 GAAGAC
BpuAI 1963 GAAGAC GAAGAC(2/6)
2758 GAAGAC
BstEII 289 GGTCACC G^GT[ACGT]ACC
1774 GGTCACC
BstPI 289 GGTCACC G^GT[ACGT]ACC
1774 GGTCACC
EagI 367 CGGCCG C^GGCCG
788 CGGCCG
EclXI 367 CGGCCG C^GGCCG
788 CGGCCG
Eco47III 354 AGCGCT AGC^GCT
939 AGCGCT
Eco52I 367 CGGCCG C^GGCCG
788 CGGCCG
EcoO65I 289 GGTCACC G^GT[ACGT]ACC
1774 GGTCACC
FseI 785 GGCCGGCC GGCCGG^CC
1793 GGCCGGCC
HincII 1529 GTTGAC GT[CT]^[GA]AC
2501 GTTGAC
HindII 1529 GTTGAC GT[CT]^[GA]AC
2501 GTTGAC
MslI 1328 CACCTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1642 CATGGGCATG
NspI 303 ACATGC [GA]CATG^[CT]
2703 GCATGC
PaeR7I 1406 CTCGAG C^TCGAG
1460 CTCGAG
PinAI 1224 ACCGGT A^CCGGT
2646 ACCGGT
PpuMI 2062 GGGTCCC [GA]G^G[AT]CC[CT]
2367 AGGACCT
SacI 2351 GAGCTC GAGCT^C
4010 GAGCTC
SbfI 323 CCTGCAGG CCTGCA^GG
1072 CCTGCAGG
SexAI 1951 ACCAGGT A^CC[AT]GGT
2370 ACCTGGT
Sse8387I 323 CCTGCAGG CCTGCA^GG
1072 CCTGCAGG
TfiI 13 GATTC G^A[AT]TC
3976 GAATC
XhoI 1406 CTCGAG C^TCGAG
1460 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BcnI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PflFI
PflMI PleI PmaCI PmlI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI