##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
Aor51HI AscI Asp718I AviII BbeI
BbrPI BciVI BfrI BsaAI BsaBI
BseAI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FspI HincII HindII HindIII HpaI
KasI KpnI MamI MluI MroI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3312 GACGTC GACGT^C
AccI 541 GTCTAC GT^[AC][GT]AC
AcyI 3312 GACGTC G[GA]^CG[CT]C
AgeI 570 ACCGGT A^CCGGT
AhdI 731 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 731 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1622 GGATCC G^GATCC
BcgI 826 GCAGCACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4195 TGATCA T^GATCA
BglII 4667 AGATCT A^GATCT
BsaHI 3312 GACGTC G[GA]^CG[CT]C
BsaI 567 GAGACC GGTCTC(1/5)
BsaWI 570 ACCGGT [AT]^CCGG[AT]
BsiEI 284 CGGCCG CG[GA][CT]^CG
BsmI 4339 GAATGC GAATGC(1/-1)
Bsp1407I 2108 TGTACA T^GTACA
BsrDI 2924 CATTGC GCAATG(2/0)
BsrGI 2108 TGTACA T^GTACA
BstAPI 2604 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 4707 TTTAAA TTT^AAA
EagI 284 CGGCCG C^GGCCG
Eam1105I 731 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 284 CGGCCG C^GGCCG
Eco52I 284 CGGCCG C^GGCCG
FbaI 4195 TGATCA T^GATCA
FseI 418 GGCCGGCC GGCCGG^CC
Hin1I 3312 GACGTC G[GA]^CG[CT]C
KspI 40 CCGCGG CCGC^GG
MfeI 2708 CAATTG C^AATTG
MunI 2708 CAATTG C^AATTG
NcoI 414 CCATGG C^CATGG
NheI 2803 GCTAGC G^CTAGC
PinAI 570 ACCGGT A^CCGGT
SacI 1698 GAGCTC GAGCT^C
SacII 40 CCGCGG CCGC^GG
SapI 683 GAAGAGC GCTCTTC(1/4)
SfiI 2607 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2108 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1558 AAATTT [GA]^AATT[CT]
4503 AAATTT
ApoI 1558 AAATTT [GA]^AATT[CT]
4503 AAATTT
AseI 1812 ATTAAT AT^TAAT
4136 ATTAAT
AsnI 1812 ATTAAT AT^TAAT
4136 ATTAAT
Asp700I 727 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2217 GAAAGATTTC
AspI 948 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2047 GACATTGTC
AvrII 536 CCTAGG C^CTAGG
2566 CCTAGG
BlnI 536 CCTAGG C^CTAGG
2566 CCTAGG
BlpI 926 GCTCAGC GC^T[ACGT]AGC
3002 GCTCAGC
Bpu1102I 926 GCTCAGC GC^T[ACGT]AGC
3002 GCTCAGC
BspHI 266 TCATGA T^CATGA
4682 TCATGA
BssSI 316 CACGAG CACGAG(-5/-1)
754 CTCGTG
CelII 926 GCTCAGC GC^T[ACGT]AGC
3002 GCTCAGC
DraIII 1903 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3964 CACCATGTG
HaeII 374 AGCGCC [GA]GCGC^[CT]
656 AGCGCC
NaeI 282 GCCGGC GCC^GGC
419 GCCGGC
NgoMIV 282 GCCGGC G^CCGGC
419 GCCGGC
PflFI 948 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2047 GACATTGTC
PflMI 1270 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4354 CCAGAAACTGG
PshBI 1812 ATTAAT AT^TAAT
4136 ATTAAT
RcaI 266 TCATGA T^CATGA
4682 TCATGA
SphI 323 GCATGC GCATG^C
643 GCATGC
Tth111I 948 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2047 GACATTGTC
Van91I 1270 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4354 CCAGAAACTGG
XbaI 2213 TCTAGA T^CTAGA
4386 TCTAGA
XmnI 727 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2217 GAAAGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI