##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccIII AclI AflIII AgeI AscI
AseI AsnI Asp700I AviII BamHI
BbeI BbrPI BcgI BsaAI BseAI
BsiWI BsmBI BspEI BspHI BspLU11I
BssHII BstBI CpoI DraI DrdI
EagI EclXI Eco52I EcoT22I FseI
FspI HincII HindII HpaI KasI
KspI MluI MroI NarI NcoI
NdeI NotI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1513 GACGTC GACGT^C
AccI 4272 GTATAC GT^[AC][GT]AC
AhdI 1150 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1324 GTGCAC G^TGCAC
Aor51HI 4076 AGCGCT AGC^GCT
ApaLI 1324 GTGCAC G^TGCAC
AspEI 1150 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1342 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4052 CCTAGG C^CTAGG
BbvCI 3584 CCTCAGC CCTCAGC(-5/-2)
BciVI 2170 GGATAC GTATCC(6/5)
BclI 1983 TGATCA T^GATCA
BglI 2222 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 401 AGATCT A^GATCT
BlnI 4052 CCTAGG C^CTAGG
BsaBI 3248 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 2210 TGTACA T^GTACA
BspDI 2005 ATCGAT AT^CGAT
BsrGI 2210 TGTACA T^GTACA
BssSI 2787 CACGAG CACGAG(-5/-1)
Bst1107I 4272 GTATAC GTA^TAC
BstAPI 2094 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 873 GGTGACC G^GT[ACGT]ACC
BstPI 873 GGTGACC G^GT[ACGT]ACC
BstZ17I 4272 GTATAC GTA^TAC
ClaI 2005 ATCGAT AT^CGAT
Eam1105I 1150 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4076 AGCGCT AGC^GCT
EcoO65I 873 GGTGACC G^GT[ACGT]ACC
EcoRI 3806 GAATTC G^AATTC
FbaI 1983 TGATCA T^GATCA
HindIII 2966 AAGCTT A^AGCTT
MamI 3248 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 393 GCTAGC G^CTAGC
NruI 168 TCGCGA TCG^CGA
PaeR7I 3019 CTCGAG C^TCGAG
PflFI 1342 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 2701 GAAGAGC GCTCTTC(1/4)
SmaI 3936 CCCGGG CCC^GGG
SspBI 2210 TGTACA T^GTACA
Tth111I 1342 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3019 CTCGAG C^TCGAG
XmaCI 3936 CCCGGG C^CCGGG
XmaI 3936 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 1836 GGTACC G^GTACC
2629 GGTACC
AcyI 1513 GACGTC G[GA]^CG[CT]C
2557 GGCGTC
AflII 3059 CTTAAG C^TTAAG
3902 CTTAAG
Asp718I 1836 GGTACC G^GTACC
2629 GGTACC
BalI 2016 TGGCCA TGG^CCA
4037 TGGCCA
BbsI 619 GAAGAC GAAGAC(2/6)
4454 GAAGAC
BfrI 3059 CTTAAG C^TTAAG
3902 CTTAAG
BpuAI 619 GAAGAC GAAGAC(2/6)
4454 GAAGAC
BsaHI 1513 GACGTC G[GA]^CG[CT]C
2557 GGCGTC
BsiEI 312 CGACCG CG[GA][CT]^CG
1554 CGACCG
BsmI 655 GAATGC GAATGC(1/-1)
4010 GCATTC
BsrDI 774 CATTGC GCAATG(2/0)
3541 GCAATG
DraIII 1327 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3785 CACTAGGTG
EaeI 2016 TGGCCA [CT]^GGCC[GA]
4037 TGGCCA
EcoRV 367 GATATC GAT^ATC
1135 GATATC
Hin1I 1513 GACGTC G[GA]^CG[CT]C
2557 GGCGTC
KpnI 1836 GGTACC GGTAC^C
2629 GGTACC
MfeI 3682 CAATTG C^AATTG
4630 CAATTG
MluNI 2016 TGGCCA TGG^CCA
4037 TGGCCA
MscI 2016 TGGCCA TGG^CCA
4037 TGGCCA
MunI 3682 CAATTG C^AATTG
4630 CAATTG
NspI 2898 ACATGC [GA]CATG^[CT]
3090 GCATGT
PshAI 288 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1555 GACCGGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII