##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
Aor51HI AscI AseI AsnI Asp700I
AviII BamHI BbvCI BciVI BclI
BfrI BglII BsaBI BsiWI BsmI
BspDI BspHI BspLU11I BsrDI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI SacI SalI ScaI
SfiI SfuI SgrAI SnaBI SspI
SwaI TfiI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1651 GACGTC GACGT^C
AhdI 3873 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2001 GTGCAC G^TGCAC
ApaLI 2001 GTGCAC G^TGCAC
AspEI 3873 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1008 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3026 GCACGTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
Bsp1407I 2328 TGTACA T^GTACA
BsrGI 2328 TGTACA T^GTACA
BssSI 2093 CACGAG CACGAG(-5/-1)
BstXI 3397 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 1747 CGGACCG CG^G[AT]CCG
DraIII 2856 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 389 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3873 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3067 GAATTC G^AATTC
PaeR7I 3765 CTCGAG C^TCGAG
PflFI 1008 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1651 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1747 CGGACCG CG^G[AT]CCG
SapI 413 GAAGAGC GCTCTTC(1/4)
SbfI 1487 CCTGCAGG CCTGCA^GG
SexAI 781 ACCAGGT A^CC[AT]GGT
SpeI 1997 ACTAGT A^CTAGT
Sse8387I 1487 CCTGCAGG CCTGCA^GG
SspBI 2328 TGTACA T^GTACA
Tth111I 1008 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1262 TCTAGA T^CTAGA
XhoI 3765 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1030 GGTACC G^GTACC
3254 GGTACC
AccIII 294 TCCGGA T^CCGGA
3063 TCCGGA
AcsI 275 GAATTT [GA]^AATT[CT]
3067 GAATTC
ApoI 275 GAATTT [GA]^AATT[CT]
3067 GAATTC
Asp718I 1030 GGTACC G^GTACC
3254 GGTACC
AvrII 1889 CCTAGG C^CTAGG
4240 CCTAGG
BlnI 1889 CCTAGG C^CTAGG
4240 CCTAGG
BlpI 351 GCTCAGC GC^T[ACGT]AGC
4182 GCTCAGC
Bpu1102I 351 GCTCAGC GC^T[ACGT]AGC
4182 GCTCAGC
BsaWI 294 TCCGGA [AT]^CCGG[AT]
3063 TCCGGA
BseAI 294 TCCGGA T^CCGGA
3063 TCCGGA
BsiEI 1173 CGGCCG CG[GA][CT]^CG
3823 CGGCCG
BsmBI 2547 CGTCTC CGTCTC(1/5)
3134 GAGACG
BspEI 294 TCCGGA T^CCGGA
3063 TCCGGA
CelII 351 GCTCAGC GC^T[ACGT]AGC
4182 GCTCAGC
EagI 1173 CGGCCG C^GGCCG
3823 CGGCCG
EclXI 1173 CGGCCG C^GGCCG
3823 CGGCCG
Eco52I 1173 CGGCCG C^GGCCG
3823 CGGCCG
HincII 265 GTCAAC GT[CT]^[GA]AC
4400 GTTGAC
HindII 265 GTCAAC GT[CT]^[GA]AC
4400 GTTGAC
KpnI 1030 GGTACC GGTAC^C
3254 GGTACC
MroI 294 TCCGGA T^CCGGA
3063 TCCGGA
MseI 452 TTAA T^TAA
1059 TTAA
NcoI 2432 CCATGG C^CATGG
3417 CCATGG
StuI 3163 AGGCCT AGG^CCT
3341 AGGCCT
Tru9I 452 TTAA T^TAA
1059 TTAA
XcmI 2982 CCAGAAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3778 CCACTAGCGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SphI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI