##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BbrPI BbsI BciVI BclI
BfrI BpuAI BsaAI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII Eam1105I EarI
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI Ksp632I KspI MaeII MluI
MroI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfiI SfuI
SnaBI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AscI 442 GGCGCGCC GG^CGCGCC
BamHI 3048 GGATCC G^GATCC
BcgI 472 GCAGGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 822 AGATCT A^GATCT
BsaBI 823 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3032 GGTCTC GGTCTC(1/5)
BsiEI 4075 CGGCCG CG[GA][CT]^CG
BsrDI 3598 CATTGC GCAATG(2/0)
BssSI 3335 CTCGTG CACGAG(-5/-1)
DrdI 4002 GACGCTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4075 CGGCCG C^GGCCG
EclXI 4075 CGGCCG C^GGCCG
Eco52I 4075 CGGCCG C^GGCCG
HindIII 2709 AAGCTT A^AGCTT
MamI 823 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 579 CAATTG C^AATTG
MunI 579 CAATTG C^AATTG
NdeI 1989 CATATG CA^TATG
NotI 4074 GCGGCCGC GC^GGCCGC
SbfI 1484 CCTGCAGG CCTGCA^GG
SgrAI 1133 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 2105 ACTAGT A^CTAGT
SphI 792 GCATGC GCATG^C
Sse8387I 1484 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BglI 2035 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3249 GCCACACAGGC
BlpI 292 GCTGAGC GC^T[ACGT]AGC
2656 GCTGAGC
Bpu1102I 292 GCTGAGC GC^T[ACGT]AGC
2656 GCTGAGC
BstAPI 2299 GCAATCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2792 GCACACCATGC
BstEII 1081 GGTGACC G^GT[ACGT]ACC
1604 GGTAACC
BstPI 1081 GGTGACC G^GT[ACGT]ACC
1604 GGTAACC
CelII 292 GCTGAGC GC^T[ACGT]AGC
2656 GCTGAGC
EcoO65I 1081 GGTGACC G^GT[ACGT]ACC
1604 GGTAACC
NaeI 1134 GCCGGC GCC^GGC
3700 GCCGGC
NgoMIV 1134 GCCGGC G^CCGGC
3700 GCCGGC
TaqI 573 TCGA T^CGA
3236 TCGA
TfiI 478 GATTC G^A[AT]TC
3241 GAATC
TthHB8I 573 TCGA T^CGA
3236 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BlnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SspBI StuI StyI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII XmaCI XmaI