##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AcyI AscI
AseI AsnI AvrII BbeI BcgI
BciVI BclI BlnI BsaHI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssSI BstBI BstEII BstPI ClaI
CpoI DrdI EagI EclXI Eco52I
EcoO65I EcoRV FbaI FseI Hin1I
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 3699 GTATAC GT^[AC][GT]AC
AccII 799 CGCG CG^CG
AflII 2090 CTTAAG C^TTAAG
AgeI 4026 ACCGGT A^CCGGT
AhdI 1445 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1601 GTGCAC G^TGCAC
AocI 3189 CCTCAGG CC^T[ACGT]AGG
Aor51HI 859 AGCGCT AGC^GCT
ApaLI 1601 GTGCAC G^TGCAC
AspEI 1445 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1724 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1638 CTCGAG C^[CT]CG[GA]G
BbrPI 2603 CACGTG CAC^GTG
BfrI 2090 CTTAAG C^TTAAG
BsaAI 2603 CACGTG [CT]AC^GT[GA]
BsaBI 1130 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1828 GGTCTC GGTCTC(1/5)
BsoBI 1638 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2166 TGTACA T^GTACA
BspHI 1248 TCATGA T^CATGA
BspLU11I 4403 ACATGT A^CATGT
BsrGI 2166 TGTACA T^GTACA
BssHII 798 GCGCGC G^CGCGC
Bst1107I 3699 GTATAC GTA^TAC
BstUI 799 CGCG CG^CG
BstZ17I 3699 GTATAC GTA^TAC
Bsu36I 3189 CCTCAGG CC^T[ACGT]AGG
DraI 1016 TTTAAA TTT^AAA
DraIII 280 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1445 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 859 AGCGCT AGC^GCT
Eco81I 3189 CCTCAGG CC^T[ACGT]AGG
EcoRI 751 GAATTC G^AATTC
HincII 4037 GTTGAC GT[CT]^[GA]AC
HindII 4037 GTTGAC GT[CT]^[GA]AC
MamI 1130 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 799 CGCG CG^CG
PaeR7I 1638 CTCGAG C^TCGAG
PflFI 1724 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4026 ACCGGT A^CCGGT
PmaCI 2603 CACGTG CAC^GTG
PmlI 2603 CACGTG CAC^GTG
RcaI 1248 TCATGA T^CATGA
ScaI 157 AGTACT AGT^ACT
SspBI 2166 TGTACA T^GTACA
SspI 4857 AATATT AAT^ATT
Tth111I 1724 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2803 TCTAGA T^CTAGA
XhoI 1638 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1814 GGTACC G^GTACC
4140 GGTACC
Asp700I 747 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
966 GAAAGTCTTC
Asp718I 1814 GGTACC G^GTACC
4140 GGTACC
AviII 538 TGCGCA TGC^GCA
1842 TGCGCA
BcnI 1966 CCGGG CC^[GC]GG
3184 CCGGG
BsmBI 174 CGTCTC CGTCTC(1/5)
4671 CGTCTC
BsmI 2988 GCATTC GAATGC(1/-1)
3195 GCATTC
BsrDI 2706 CATTGC GCAATG(2/0)
3801 CATTGC
BsrFI 3419 GCCGGT [GA]^CCGG[CT]
4026 ACCGGT
BstXI 985 CCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2569 CCAGCTCCTTGG
Cfr10I 3419 GCCGGT [GA]^CCGG[CT]
4026 ACCGGT
EcoT22I 1050 ATGCAT ATGCA^T
1835 ATGCAT
FspI 538 TGCGCA TGC^GCA
1842 TGCGCA
HindIII 1847 AAGCTT A^AGCTT
3994 AAGCTT
KpnI 1814 GGTACC GGTAC^C
4140 GGTACC
MaeII 344 ACGT A^CGT
2604 ACGT
NciI 1966 CCGGG CC^[GC]GG
3184 CCGGG
NdeI 368 CATATG CA^TATG
1838 CATATG
NsiI 1050 ATGCAT ATGCA^T
1835 ATGCAT
SacI 2653 GAGCTC GAGCT^C
3764 GAGCTC
SbfI 947 CCTGCAGG CCTGCA^GG
2410 CCTGCAGG
SexAI 724 ACCAGGT A^CC[AT]GGT
1995 ACCAGGT
Sse8387I 947 CCTGCAGG CCTGCA^GG
2410 CCTGCAGG
StuI 99 AGGCCT AGG^CCT
1734 AGGCCT
XcmI 2126 CCAGCGGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3051 CCAGCCAAGTGCTGG
XmnI 747 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
966 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII