##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccI AclI AflII Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbvCI
BciVI BfrI BlnI BsaBI BsiWI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI EcoRI EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1583 GACGTC GACGT^C
AccIII 4085 TCCGGA T^CCGGA
AcsI 974 GAATTT [GA]^AATT[CT]
AgeI 3372 ACCGGT A^CCGGT
AlwI 670 GATCC GGATC(4/5)
Aor51HI 3207 AGCGCT AGC^GCT
ApoI 974 GAATTT [GA]^AATT[CT]
BcgI 3599 GCATCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1017 TGATCA T^GATCA
BglII 1048 AGATCT A^GATCT
BsaI 95 GAGACC GGTCTC(1/5)
BseAI 4085 TCCGGA T^CCGGA
BsmI 2265 GCATTC GAATGC(1/-1)
BspEI 4085 TCCGGA T^CCGGA
BspLU11I 3413 ACATGT A^CATGT
BstEII 3733 GGTGACC G^GT[ACGT]ACC
BstPI 3733 GGTGACC G^GT[ACGT]ACC
BstYI 1048 AGATCT [GA]^GATC[CT]
EarI 92 GAAGAG CTCTTC(1/4)
Eco47III 3207 AGCGCT AGC^GCT
EcoO65I 3733 GGTGACC G^GT[ACGT]ACC
FbaI 1017 TGATCA T^GATCA
HincII 1142 GTCAAC GT[CT]^[GA]AC
HindII 1142 GTCAAC GT[CT]^[GA]AC
Ksp632I 92 GAAGAG CTCTTC(1/4)
KspI 866 CCGCGG CCGC^GG
MflI 1048 AGATCT [GA]^GATC[CT]
MroI 4085 TCCGGA T^CCGGA
NspI 3413 ACATGT [GA]CATG^[CT]
PinAI 3372 ACCGGT A^CCGGT
SacII 866 CCGCGG CCGC^GG
XhoII 1048 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 3413 ACATGT A^C[GA][CT]GT
3945 ACGTGT
BbeI 1073 GGCGCC GGCGC^C
3727 GGCGCC
BbsI 445 GAAGAC GAAGAC(2/6)
4232 GAAGAC
BpuAI 445 GAAGAC GAAGAC(2/6)
4232 GAAGAC
BsrBI 1216 CCGCTC CCGCTC(-3/-3)
4274 GAGCGG
BssSI 1955 CTCGTG CACGAG(-5/-1)
3388 CTCGTG
BstAPI 1738 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3141 GCACACGGTGC
DrdI 1847 GACAGGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2181 GACCGGGGAGTC
KasI 1073 GGCGCC G^GCGCC
3727 GGCGCC
NarI 1073 GGCGCC GG^CGCC
3727 GGCGCC
PshAI 836 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1768 GACACTGGTC
SexAI 1961 ACCAGGT A^CC[AT]GGT
3813 ACCAGGT
SfoI 1073 GGCGCC GGC^GCC
3727 GGCGCC
SgrAI 3371 CACCGGTG C[GA]^CCGG[CT]G
3729 CGCCGGTG
TfiI 1521 GATTC G^A[AT]TC
4279 GATTC
Tsp509I 975 AATT ^AATT
4002 AATT
XbaI 2069 TCTAGA T^CTAGA
3913 TCTAGA
XcmI 3321 CCAGAGTCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3411 CCACATGTGGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwNI AocI ApaI AspEI
AspHI AspI AvaI AvaII BalI
BanI BanII BbrPI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PaeR7I PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI