##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AspI AvrII BalI BamHI
BbeI BbvCI BclI BfrI BglI
BlnI BsaI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspEI BsrFI BsrGI
BssHII BstBI BstEII BstPI Cfr10I
CpoI DraIII DrdI EagI EclXI
Eco52I EcoNI EcoO65I EcoRI FbaI
FseI HindIII KasI KspI MluNI
MroI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PshAI Psp1406I
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 2870 GTGCAC G^TGCAC
Aor51HI 3283 AGCGCT AGC^GCT
ApaLI 2870 GTGCAC G^TGCAC
AseI 1651 ATTAAT AT^TAAT
AsnI 1651 ATTAAT AT^TAAT
Asp700I 2738 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1053 TGCGCA TGC^GCA
BbrPI 1045 CACGTG CAC^GTG
BciVI 384 GTATCC GTATCC(6/5)
BlpI 201 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 201 GCTCAGC GC^T[ACGT]AGC
BsaBI 1771 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3279 GTGCAG GTGCAG(16/14)
BsiEI 2953 CGATCG CG[GA][CT]^CG
BspDI 4342 ATCGAT AT^CGAT
BspLU11I 1735 ACATGT A^CATGT
Bst1107I 1442 GTATAC GTA^TAC
BstAPI 206 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1442 GTATAC GTA^TAC
CelII 201 GCTCAGC GC^T[ACGT]AGC
ClaI 4342 ATCGAT AT^CGAT
Eco47III 3283 AGCGCT AGC^GCT
EcoRV 1786 GATATC GAT^ATC
FspI 1053 TGCGCA TGC^GCA
HaeII 3283 AGCGCT [GA]GCGC^[CT]
HincII 2948 GTTAAC GT[CT]^[GA]AC
HindII 2948 GTTAAC GT[CT]^[GA]AC
HpaI 2948 GTTAAC GTT^AAC
MamI 1771 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1309 CAATTG C^AATTG
MluI 2319 ACGCGT A^CGCGT
MunI 1309 CAATTG C^AATTG
PacI 4432 TTAATTAA TTAAT^TAA
PmaCI 1045 CACGTG CAC^GTG
PmeI 3837 GTTTAAAC GTTT^AAAC
PmlI 1045 CACGTG CAC^GTG
PshBI 1651 ATTAAT AT^TAAT
PvuI 2953 CGATCG CGAT^CG
SacI 199 GAGCTC GAGCT^C
ScaI 3089 AGTACT AGT^ACT
SwaI 4294 ATTTAAAT ATTT^AAAT
XmnI 2738 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1304 GGTACC G^GTACC
4114 GGTACC
AccII 2320 CGCG CG^CG
2970 CGCG
AcyI 2814 GACGCC G[GA]^CG[CT]C
3230 GGCGTC
AhdI 625 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3210 GACATGATGTC
AlwNI 3127 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
3654 CAGGTTCTG
AocI 2618 CCTCAGG CC^T[ACGT]AGG
3124 CCTCAGG
ApaI 310 GGGCCC GGGCC^C
722 GGGCCC
Asp718I 1304 GGTACC G^GTACC
4114 GGTACC
AspEI 625 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3210 GACATGATGTC
BbsI 62 GAAGAC GAAGAC(2/6)
3207 GAAGAC
BcgI 1214 GCACATCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3141 GCATGGCTTTCG
BglII 23 AGATCT A^GATCT
1274 AGATCT
BpuAI 62 GAAGAC GAAGAC(2/6)
3207 GAAGAC
BsaAI 1045 CACGTG [CT]AC^GT[GA]
1449 TACGTG
BsaHI 2814 GACGCC G[GA]^CG[CT]C
3230 GGCGTC
BspHI 3293 TCATGA T^CATGA
4132 TCATGA
BspMI 987 GCAGGT ACCTGC(4/8)
1017 GCAGGT
BsrBI 1205 GAGCGG CCGCTC(-3/-3)
3326 GAGCGG
BsrDI 1257 CATTGC GCAATG(2/0)
2389 CATTGC
BssSI 226 CACGAG CACGAG(-5/-1)
4361 CACGAG
BstUI 2320 CGCG CG^CG
2970 CGCG
BstXI 751 CCAGATGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
775 CCAGCCATGTGG
Bsu36I 2618 CCTCAGG CC^T[ACGT]AGG
3124 CCTCAGG
Eam1105I 625 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3210 GACATGATGTC
EarI 3622 CTCTTC CTCTTC(1/4)
4322 CTCTTC
Eco81I 2618 CCTCAGG CC^T[ACGT]AGG
3124 CCTCAGG
Hin1I 2814 GACGCC G[GA]^CG[CT]C
3230 GGCGTC
KpnI 1304 GGTACC GGTAC^C
4114 GGTACC
Ksp632I 3622 CTCTTC CTCTTC(1/4)
4322 CTCTTC
MvnI 2320 CGCG CG^CG
2970 CGCG
NcoI 2657 CCATGG C^CATGG
2865 CCATGG
PstI 3387 CTGCAG CTGCA^G
4181 CTGCAG
PvuII 813 CAGCTG CAG^CTG
4278 CAGCTG
RcaI 3293 TCATGA T^CATGA
4132 TCATGA
StuI 2291 AGGCCT AGG^CCT
4222 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI