##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp700I AviII AvrII BbeI
BbrPI BcgI BfrI BlnI BsaAI
BsaBI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
FseI FspI HaeII KasI KspI
MamI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 696 GGTACC G^GTACC
AcyI 2779 GGCGTC G[GA]^CG[CT]C
AflIII 3051 ACATGT A^C[GA][CT]GT
AhdI 1532 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2869 CCTCAGG CC^T[ACGT]AGG
AseI 3716 ATTAAT AT^TAAT
AsnI 3716 ATTAAT AT^TAAT
Asp718I 696 GGTACC G^GTACC
AspEI 1532 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3370 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3303 CCCGGG C^[CT]CG[GA]G
BamHI 936 GGATCC G^GATCC
BbsI 575 GAAGAC GAAGAC(2/6)
BglII 74 AGATCT A^GATCT
BlpI 1845 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1845 GCTCAGC GC^T[ACGT]AGC
BpuAI 575 GAAGAC GAAGAC(2/6)
BsaHI 2779 GGCGTC G[GA]^CG[CT]C
BsaWI 3501 TCCGGT [AT]^CCGG[AT]
BsmBI 3375 CGTCTC CGTCTC(1/5)
BsoBI 3303 CCCGGG C^[CT]CG[GA]G
BspHI 3020 TCATGA T^CATGA
BspLU11I 3051 ACATGT A^CATGT
BsrDI 2159 CATTGC GCAATG(2/0)
BssSI 2118 CTCGTG CACGAG(-5/-1)
BstEII 1691 GGTGACC G^GT[ACGT]ACC
BstPI 1691 GGTGACC G^GT[ACGT]ACC
Bsu36I 2869 CCTCAGG CC^T[ACGT]AGG
CelII 1845 GCTCAGC GC^T[ACGT]AGC
CfoI 3336 GCGC GCG^C
DsaI 1814 CCATGG C^C[GA][CT]GG
Eam1105I 1532 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2869 CCTCAGG CC^T[ACGT]AGG
EcoNI 1388 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1691 GGTGACC G^GT[ACGT]ACC
EcoRI 2224 GAATTC G^AATTC
EcoRV 894 GATATC GAT^ATC
EcoT22I 2752 ATGCAT ATGCA^T
HgaI 2780 GCGTC GACGC(5/10)
HhaI 3336 GCGC GCG^C
Hin1I 2779 GGCGTC G[GA]^CG[CT]C
HinP1I 3336 GCGC G^CGC
HindIII 615 AAGCTT A^AGCTT
HpaI 3458 GTTAAC GTT^AAC
KpnI 696 GGTACC GGTAC^C
MfeI 637 CAATTG C^AATTG
MunI 637 CAATTG C^AATTG
NcoI 1814 CCATGG C^CATGG
NdeI 1324 CATATG CA^TATG
NsiI 2752 ATGCAT ATGCA^T
PacI 4349 TTAATTAA TTAAT^TAA
PflFI 3370 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3716 ATTAAT AT^TAAT
RcaI 3020 TCATGA T^CATGA
ScaI 2089 AGTACT AGT^ACT
SmaI 3303 CCCGGG CCC^GGG
SphI 2083 GCATGC GCATG^C
StuI 1422 AGGCCT AGG^CCT
Tth111I 3370 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4028 TCTAGA T^CTAGA
XmaCI 3303 CCCGGG C^CCGGG
XmaI 3303 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2719 GTCTAC GT^[AC][GT]AC
3618 GTAGAC
AccII 2516 CGCG CG^CG
3335 CGCG
AspHI 1678 GTGCTC G[AT]GC[AT]^C
2371 GTGCTC
BalI 1641 TGGCCA TGG^CCA
1811 TGGCCA
BanI 696 GGTACC G^G[CT][GA]CC
2459 GGCACC
BclI 1943 TGATCA T^GATCA
1965 TGATCA
BcnI 3303 CCCGG CC^[GC]GG
3304 CCGGG
BglI 1153 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1813 GCCATGGTGGC
BsaI 865 GAGACC GGTCTC(1/5)
1921 GAGACC
BseRI 2634 CTCCTC GAGGAG(10/8)
3937 CTCCTC
BsgI 3342 CTGCAC GTGCAG(16/14)
570 GTGCAG
BsiHKAI 1678 GTGCTC G[AT]GC[AT]^C
2371 GTGCTC
BstUI 2516 CGCG CG^CG
3335 CGCG
EaeI 1641 TGGCCA [CT]^GGCC[GA]
1811 TGGCCA
FbaI 1943 TGATCA T^GATCA
1965 TGATCA
MluNI 1641 TGGCCA TGG^CCA
1811 TGGCCA
MscI 1641 TGGCCA TGG^CCA
1811 TGGCCA
MvnI 2516 CGCG CG^CG
3335 CGCG
NciI 3303 CCCGG CC^[GC]GG
3304 CCGGG
NspI 2083 GCATGC [GA]CATG^[CT]
3051 ACATGT
XcmI 1799 CCATGAACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1870 CCAAGGCTGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvCI BbvI
BciVI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII