##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AviII BamHI
BciVI BfrI BsaBI BsiEI BsiWI
BsmI Bsp1407I BspDI BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FseI
FspI KpnI KspI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 1294 CGCG CG^CG
AcsI 918 GAATTC [GA]^AATT[CT]
AlwI 3888 GGATC GGATC(4/5)
ApoI 918 GAATTC [GA]^AATT[CT]
AvrII 3154 CCTAGG C^CTAGG
BbeI 3163 GGCGCC GGCGC^C
BbrPI 2573 CACGTG CAC^GTG
BcgI 2642 CGAGACAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 937 TGATCA T^GATCA
BglII 2948 AGATCT A^GATCT
BlnI 3154 CCTAGG C^CTAGG
BlpI 3696 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3696 GCTCAGC GC^T[ACGT]AGC
BsaAI 2573 CACGTG [CT]AC^GT[GA]
BsmBI 1332 CGTCTC CGTCTC(1/5)
BspHI 110 TCATGA T^CATGA
BspLU11I 1228 ACATGT A^CATGT
BsrFI 856 ACCGGC [GA]^CCGG[CT]
BssSI 941 CACGAG CACGAG(-5/-1)
BstUI 1294 CGCG CG^CG
CelII 3696 GCTCAGC GC^T[ACGT]AGC
Cfr10I 856 ACCGGC [GA]^CCGG[CT]
EcoRI 918 GAATTC G^AATTC
FbaI 937 TGATCA T^GATCA
HpaI 789 GTTAAC GTT^AAC
KasI 3163 GGCGCC G^GCGCC
MvnI 1294 CGCG CG^CG
NarI 3163 GGCGCC GG^CGCC
PmaCI 2573 CACGTG CAC^GTG
PmlI 2573 CACGTG CAC^GTG
RcaI 110 TCATGA T^CATGA
SapI 3806 GCTCTTC GCTCTTC(1/4)
ScaI 414 AGTACT AGT^ACT
SfoI 3163 GGCGCC GGC^GCC
SphI 370 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 732 GACGTC GACGT^C
1330 GACGTC
AccIII 710 TCCGGA T^CCGGA
2503 TCCGGA
AflIII 1228 ACATGT A^C[GA][CT]GT
2574 ACGTGT
Aor51HI 610 AGCGCT AGC^GCT
3937 AGCGCT
BbsI 1011 GTCTTC GAAGAC(2/6)
2327 GTCTTC
BpuAI 1011 GTCTTC GAAGAC(2/6)
2327 GTCTTC
BsaWI 710 TCCGGA [AT]^CCGG[AT]
2503 TCCGGA
BseAI 710 TCCGGA T^CCGGA
2503 TCCGGA
BspEI 710 TCCGGA T^CCGGA
2503 TCCGGA
BstYI 2948 AGATCT [GA]^GATC[CT]
3888 GGATCT
Eco47III 610 AGCGCT AGC^GCT
3937 AGCGCT
HincII 789 GTTAAC GT[CT]^[GA]AC
2540 GTCAAC
HindII 789 GTTAAC GT[CT]^[GA]AC
2540 GTCAAC
MflI 2948 AGATCT [GA]^GATC[CT]
3888 GGATCT
MroI 710 TCCGGA T^CCGGA
2503 TCCGGA
MslI 2531 CATCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4469 CATTGTGGTG
SbfI 1688 CCTGCAGG CCTGCA^GG
2675 CCTGCAGG
SmaI 4025 CCCGGG CCC^GGG
4413 CCCGGG
Sse8387I 1688 CCTGCAGG CCTGCA^GG
2675 CCTGCAGG
StuI 2307 AGGCCT AGG^CCT
3248 AGGCCT
TaqI 3231 TCGA T^CGA
3649 TCGA
TthHB8I 3231 TCGA T^CGA
3649 TCGA
XhoII 2948 AGATCT [GA]^GATC[CT]
3888 GGATCT
XmaCI 4025 CCCGGG C^CCGGG
4413 CCCGGG
XmaI 4025 CCCGGG C^CCGGG
4413 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AocI ApaI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstXI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I Van91I
XcmI XmnI