##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AclI AflII AflIII AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BbrPI BbsI BciVI BclI BfrI
BpuAI BsaBI BsiWI BspDI BspLU11I
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FbaI FseI FspI HindIII
KspI MamI MfeI MluI MunI
NheI NotI NruI PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII ScaI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3347 GACGTC GACGT^C
Acc65I 241 GGTACC G^GTACC
AccIII 839 TCCGGA T^CCGGA
AcsI 847 GAATTC [GA]^AATT[CT]
AgeI 3114 ACCGGT A^CCGGT
ApoI 847 GAATTC [GA]^AATT[CT]
Asp700I 843 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 241 GGTACC G^GTACC
AvrII 2586 CCTAGG C^CTAGG
BcgI 653 GCATGCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2586 CCTAGG C^CTAGG
BsaAI 991 TACGTG [CT]AC^GT[GA]
BseAI 839 TCCGGA T^CCGGA
BsiEI 3112 CGACCG CG[GA][CT]^CG
BsmI 2989 GAATGC GAATGC(1/-1)
Bsp1407I 1064 TGTACA T^GTACA
BspEI 839 TCCGGA T^CCGGA
BspHI 425 TCATGA T^CATGA
BsrBI 2167 GAGCGG CCGCTC(-3/-3)
BsrDI 2466 CATTGC GCAATG(2/0)
BsrGI 1064 TGTACA T^GTACA
BstBI 1219 TTCGAA TT^CGAA
DraIII 2338 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 847 GAATTC G^AATTC
HpaI 1276 GTTAAC GTT^AAC
KpnI 241 GGTACC GGTAC^C
MroI 839 TCCGGA T^CCGGA
MseI 1277 TTAA T^TAA
NaeI 459 GCCGGC GCC^GGC
NgoMIV 459 GCCGGC G^CCGGC
NspI 653 GCATGC [GA]CATG^[CT]
NspV 1219 TTCGAA TT^CGAA
PflMI 753 CCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3114 ACCGGT A^CCGGT
RcaI 425 TCATGA T^CATGA
SalI 1966 GTCGAC G^TCGAC
SbfI 1509 CCTGCAGG CCTGCA^GG
SfiI 3253 GGCCAAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1219 TTCGAA TT^CGAA
SphI 653 GCATGC GCATG^C
Sse8387I 1509 CCTGCAGG CCTGCA^GG
SspBI 1064 TGTACA T^GTACA
StuI 2039 AGGCCT AGG^CCT
Tru9I 1277 TTAA T^TAA
Van91I 753 CCACATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 843 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1966 GTCGAC GT^[AC][GT]AC
2113 GTCTAC
Alw44I 219 GTGCAC G^TGCAC
2193 GTGCAC
ApaLI 219 GTGCAC G^TGCAC
2193 GTGCAC
AspI 880 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2107 GACGAGGTC
BamHI 377 GGATCC G^GATCC
3299 GGATCC
BbeI 1003 GGCGCC GGCGC^C
4056 GGCGCC
BsaI 3515 GAGACC GGTCTC(1/5)
3754 GAGACC
BsmBI 2370 CGTCTC CGTCTC(1/5)
495 GAGACG
DrdI 1564 GACCTGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3907 GACCCCCCCGTC
EcoT22I 1405 ATGCAT ATGCA^T
3556 ATGCAT
HincII 1276 GTTAAC GT[CT]^[GA]AC
1966 GTCGAC
HindII 1276 GTTAAC GT[CT]^[GA]AC
1966 GTCGAC
KasI 1003 GGCGCC G^GCGCC
4056 GGCGCC
NarI 1003 GGCGCC GG^CGCC
4056 GGCGCC
NdeI 200 CATATG CA^TATG
3957 CATATG
NsiI 1405 ATGCAT ATGCA^T
3556 ATGCAT
PflFI 880 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2107 GACGAGGTC
SacI 2056 GAGCTC GAGCT^C
3529 GAGCTC
SexAI 2339 ACCAGGT A^CC[AT]GGT
2934 ACCAGGT
SfoI 1003 GGCGCC GGC^GCC
4056 GGCGCC
SmaI 368 CCCGGG CCC^GGG
872 CCCGGG
SmlI 1945 CTCAAG C^T[CT][GA]AG
2863 CTTGAG
Tsp509I 848 AATT ^AATT
2955 AATT
Tth111I 880 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2107 GACGAGGTC
XmaCI 368 CCCGGG C^CCGGG
872 CCCGGG
XmaI 368 CCCGGG C^CCGGG
872 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XhoII